Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00719  |
| T6SS type | Type i1 |
| Strain | Vibrio parahaemolyticus BB22OP |
| Replicon (RefSeq) | chromosome I (NC_019955) |
| Location | 1464178..1493343 |
| Function | anti-bacterial |
| Comment | BB22OP T6SS1 is a functional antibacterial system. BB22OP T6SS1 was active under warm marine-like conditions (3% NaCl, 30°C) in the presence of surface, as evident by secretion of the hallmark T6SS1 component VgrG1. However, inactivation of BB22OP T6SS1 by deletion of the conserved structural component, hcp1, had only a minor effect on BB22OP's ability to kill E. coli prey during competition. |
| References | [1] Fridman CM, Keppel K, Gerlic M, Bosis E, Salomon D. et al (2020) A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors.. Nat Commun. 11(1):1085. [PudMed:32109231] [2] Gode-Potratz CJ et al (2011) Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 193(16):4224-37. [PudMed:21705592] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | FHA | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| VPBB_RS06655 | 1462563..1464191 | - | 1629 | WP_015296664.1 | sigma-54 dependent transcriptional regulator | ||
| T6CP007565 | TssH | VPBB_RS06660 (tssH) | 1464178..1466850 | - | 2673 | WP_015296665.1 | type VI secretion system ATPase TssH |
| T6CP009233 | TssD | VPBB_RS06665 | 1467305..1467823 | + | 519 | WP_005480650.1 | Hcp family type VI secretion system effector |
| T6CP007566 | TssI | VPBB_RS06670 (tssI) | 1467896..1469974 | + | 2079 | WP_015296666.1 | type VI secretion system tip protein VgrG |
| VPBB_RS06675 | 1469974..1470459 | + | 486 | WP_005480759.1 | hypothetical protein | ||
| VPBB_RS06680 | 1470537..1471856 | + | 1320 | WP_015296667.1 | hypothetical protein | ||
| VPBB_RS06685 | 1471837..1472829 | + | 993 | WP_015296668.1 | DUF2169 domain-containing protein | ||
| VPBB_RS06690 | 1472822..1473766 | + | 945 | WP_015296669.1 | hypothetical protein | ||
| VPBB_RS06695 | 1474001..1476004 | + | 2004 | WP_015296670.1 | protein kinase family protein | ||
| T6CP009232 | TssA | VPBB_RS06700 (tssA) | 1476004..1477578 | + | 1575 | WP_015296671.1 | type VI secretion system protein TssA |
| T6CP007567 | TssB | VPBB_RS06705 (tssB) | 1477605..1478111 | + | 507 | WP_005480589.1 | type VI secretion system contractile sheath small subunit |
| T6CP007568 | TssC | VPBB_RS06710 (tssC) | 1478120..1479595 | + | 1476 | WP_005480731.1 | type VI secretion system contractile sheath large subunit |
| T6CP009234 | TssE | VPBB_RS06715 (tssE) | 1479657..1480067 | + | 411 | WP_005480585.1 | type VI secretion system baseplate subunit TssE |
| T6CP007569 | TssF | VPBB_RS06720 (tssF) | 1480078..1481826 | + | 1749 | WP_015296672.1 | type VI secretion system baseplate subunit TssF |
| T6CP007570 | TssG | VPBB_RS06725 (tssG) | 1481823..1482821 | + | 999 | WP_015296673.1 | type VI secretion system baseplate subunit TssG |
| VPBB_RS06730 | 1482885..1483337 | - | 453 | WP_005480668.1 | Lrp/AsnC family transcriptional regulator | ||
| T6CP007571 | TssM | VPBB_RS06735 (tssM) | 1483370..1486759 | - | 3390 | WP_015296675.1 | type VI secretion system membrane subunit TssM |
| T6CP009649 | TssA | VPBB_RS06740 | 1486781..1488076 | - | 1296 | WP_015296676.1 | type VI secretion system ImpA family N-terminal domain-containing protein |
| VPBB_RS06745 | 1488596..1489195 | + | 600 | WP_005480745.1 | hypothetical protein | ||
| T6CP012218 | FHA | VPBB_RS06750 | 1489205..1490725 | + | 1521 | WP_015296677.1 | FHA domain-containing protein |
| T6CP007572 | TssJ | VPBB_RS06755 (tssJ) | 1490718..1491218 | + | 501 | WP_011105829.1 | type VI secretion system lipoprotein TssJ |
| T6CP007573 | TssK | VPBB_RS06760 (tssK) | 1491230..1492555 | + | 1326 | WP_015296678.1 | type VI secretion system baseplate subunit TssK |
| T6CP007574 | TssL | VPBB_RS06765 (icmH) | 1492552..1493343 | + | 792 | WP_005495177.1 | type IVB secretion system protein IcmH/DotU |
| VPBB_RS06770 | 1493493..1495016 | + | 1524 | WP_015296679.1 | DUF4150 domain-containing protein | ||
| VPBB_RS06775 | 1495016..1495897 | + | 882 | WP_015296680.1 | AHH domain-containing protein | ||
| VPBB_RS06780 | 1495902..1496507 | + | 606 | WP_029826410.1 | hypothetical protein | ||
| VPBB_RS06785 | 1496510..1497001 | + | 492 | WP_015296682.1 | hypothetical protein | ||
| VPBB_RS06790 | 1497186..1497479 | + | 294 | WP_005480582.1 | hypothetical protein | ||
| VPBB_RS06795 | 1497639..1498241 | + | 603 | WP_015296684.1 | 2OG-Fe(II) oxygenase |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0329 |
VPBB_RS05425 (H-NS) | protein | Transcription regulator | Vibrio parahaemolyticus BB22OP | chromosome (NC_019955) | 1170475..1170882 (-) | 505109423 | VgrG1 expression and secretion (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01832 |
VPBB_RS15030 (Tme1) | Tme | IMU00352 |
Vibrio parahaemolyticus BB22OP | chromosome (NC_019955) | 3247654..3248586 (+) | 505110423 |
| EFF01830 |
VPBB_RS06670 (VgrG1) | - | - | Vibrio parahaemolyticus BB22OP | chromosome (NC_019955) | 1467896..1469974 (+) | 505109564 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU00352 |
VPBB_RS15035 (Tmi1) | EFF01832 |
Vibrio parahaemolyticus BB22OP | chromosome (NC_019955) | 3248576..3249004 (+) | 491520929 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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