Detailed information of component protein


Summary

Component ID T6CP009649
Component protein type TssA
T6SS ID (Type) T6SS00719 experimental (Type i1)
Strain Vibrio parahaemolyticus BB22OP
Replicon chromosome I
Sequence Protein sequence (432 a.a.); Nucleotide sequence (1296 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VPBB_RS06740
Coordinate (Strand) 1486781..1488076 (-)
NCBI ID WP_015296676.1
RefSeq NC_019955
Uniprot ID UPI0002A5827C
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2e-24 9..119
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-230 MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGLKGLVEGLNSVLDLCVVYWFSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIKAPSFGRIRRPLNQWIEEIKEEKAKQEKTSQTKPAANPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSR
Transmembrane helix 231-250 FIIYTMIGLLIIAFTSHFIY
Inside 251-431 KQHQYTMLKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLTTDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKNSQRKEIERSLLLLNAYLYKINQLQAAEKDNK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9452 - -


  Protein sequence: 432 a.a.    

>T6CP009649 NC_019955:c1488076-1486781 [Vibrio parahaemolyticus BB22OP] [TssA]
MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGL
KGLVEGLNSVLDLCVVYWFSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIK
APSFGRIRRPLNQWIEEIKEEKAKQEKTSQTKPAANPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSRFIIYTMIGLL
IIAFTSHFIYKQHQYTMLKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLT
TDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKN
SQRKEIERSLLLLNAYLYKINQLQAAEKDNK*

  Nucleotide sequence: 1296 bp    

>T6CP009649 NC_019955:c1488076-1486781 [Vibrio parahaemolyticus BB22OP] [TssA]
ATGTTTTTCAGCGACTTCACAAGAAGACCGATCGATGAAAGTAATCCAAGCGGTACCAATCCAAATGGCTTAGACGACTT
TGATGAAATAAAACGTCAAATAAATGGCTTGAACAAAGTAACAGGCCGTGTGTCATGGAAGACAGTTCAGACACTATCAA
AAGAAATCCTTAGAACGAAAGGCAAAGACTTTCGCTGTAGCTGTTACTTTACTGTGGCAGCCATTAATAATGACGGACTA
AAAGGCCTAGTTGAGGGCTTAAATTCTGTGCTCGACTTGTGTGTTGTTTATTGGTTCTCCGCCTATCCTGAGTACACAAA
AGCTAATGCCCGTATTAGTGCGATTGAATGGATGGTTGAGTACACTGAAAAACGAATGAAGAAACACCGAGTCCTGCCGG
AAGAACTCCCCCTAATTGAAGCGGCTCATCAGCTTTGTCTACGTATCGAAGAGGAACTTCGCCTTCACTATGGTATTAAG
GCACCTTCTTTTGGACGTATTCGTCGTCCCCTAAATCAGTGGATTGAAGAGATTAAAGAAGAAAAGGCGAAACAAGAGAA
AACCTCACAAACAAAACCTGCGGCAAATCCTCCTTCAGCAGGCATCCGAGTCGAAATAGCACCGCCTAATAGACCTGTTC
AAAAGAAAGTAGAGCAGCCTAAAGTGGAAGAAGAGGCCAAATCAAGCCGATTCATTATCTATACAATGATCGGATTGCTG
ATCATAGCTTTTACGTCCCACTTCATATACAAACAGCATCAATACACAATGCTCAAGGAAAGAATTGGGTCAGCATCACT
TACTGAATTAGTGACGATTGTTAACTCGCTCAGTGTCGAAAACAAAAATTACCAAGATGACTTGCGCTCTGTCACGGTTG
AGCGTCTTCATACTTTGATGTCAGGTTGGACATTAGATCCAGTCAAAGTGAGCCAGGTCAATACCATTGAACAGTTAACG
ACTGACCTGACCAAGCTCTATCCAGACTCATCCTCTGCACAACAACTTCGCGATAATTTTTTAGGCCAACGTTCTTATTT
TGAAGATGAATTTGAGGCTATATACAAACGTTTTGCATATGCCAGAACGGTCTTTGCCAACATTGCAAAACAAAGTGCAG
ACAAGGACGCTAAGAAAGCATATGAGTACAGCAATTCGCTATTTCCCCTTCTTGGGCGAATTGAATATGCAGAAAAGAAC
TCTCAGCGAAAGGAAATTGAACGATCACTTCTTTTGCTGAACGCTTACCTATATAAAATCAACCAGCTACAAGCTGCAGA
AAAAGACAATAAATAA