SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007571
Component protein type	TssM
T6SS ID (Type)	T6SS00719 (Type i1)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome I
Sequence	Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS06735
Coordinate (Strand)	1483370..1486759 (-)
NCBI ID	WP_015296675.1
RefSeq	NC_019955
Uniprot ID	UPI0002A556AD
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	1.7e-54	154..408
IcmF-related	PF06761.14	2.9e-42	496..755
IcmF_C	PF06744.14	6.7e-19	1004..1107

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-11	MFKQKILRNPV
Transmembrane helix	12-34	VVSTIVALVLAAIFAAVYLFIFE
Outside	35-38	DADK
Transmembrane helix	39-61	LYLWVGLGVITGFYFTFQLSLWL
Inside	62-401	KKKNSSKVQRLETEEEALSMVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLWNTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSSSLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDISEKQAILAFPKQFELCQREISYVLERLCDTNNGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQRQ
Transmembrane helix	402-424	SHLAMLASVVLLAGGTYFFVTTL
Outside	425-1129	DSNLRVINQLLNIDEKVQTEQEASFNQLLMSATRAIQPSYNAWLEGSRALDEEIVPLNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTATLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLSDKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEPLDDNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSNYVTPTKEEQESFKNQVRKKYTDDYINYWRNALSELKIQSYSNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQAFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSWLLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTNLWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRASLKISDTDINYYHGPSRIREMEWPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAELYNFTLPESI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9979	-	-

Protein sequence: 1130 a.a.

						>T6CP007571 NC_019955:c1486759-1483370 [Vibrio parahaemolyticus BB22OP] [TssM]
MFKQKILRNPVVVSTIVALVLAAIFAAVYLFIFEDADKLYLWVGLGVITGFYFTFQLSLWLKKKNSSKVQRLETEEEALS
MVIRPLLSNAGKKPIYLMVGNKGAGRRQFLFNSSAIKPMDRSRTAKNDFFEWYESDGAVYVKPDQRLIFQEVSSSDASLW
NTFIDEVIRHRPRKPFSGCLFFVDFEFMIVSEPEQKDYTMTALLQRLNSITEKTSSALPVYLMMTKLDKLDGFKEYVHSS
SLKTRIEFLSIPLKEAKGVLTEYYLDSFHNLVKVLESNALDASANSTDISEKQAILAFPKQFELCQREISYVLERLCDTN
NGVYSLDIREIFFCSSLQGGRKYNLLAKSCSNYFNLPIIASEHTQLTETPYFSRFLLDSQILPESDFAGENKTYLRMIQR
QSHLAMLASVVLLAGGTYFFVTTLDSNLRVINQLLNIDEKVQTEQEASFNQLLMSATRAIQPSYNAWLEGSRALDEEIVP
LNISRLNESTKLAYEALIKEVAQQLMPVIEQGYRFELTHNQHDVTATLPLLKGYLMLKDPTKRDIQFLRRQTLQTLTDLS
DKPDVVNQAMRYLDAYFRTQFSPVDINMDVVRATRRSLLANSNVDLVYAGILNQADAIDLGTLDLQRAVGFEFNNVFNEP
LDDNRLVIDKIYTSTGFSTFYRPRVDLMSKQVISDNWVLGLSNYVTPTKEEQESFKNQVRKKYTDDYINYWRNALSELKI
QSYSNVGDLTNAIDLISGPSSPMTTVLKQVYANTQFSPVGEKNVLISKVDPKLAEVANSASEAVEEVVQPDYLLMKRVEQ
AFHLLNQLQVSETPNSPTPWDETIAALSRVRTYMKDIADAPDPQMAALAAAQHRMSSSEADPLIKLKQIAQKSPEPVRSW
LLDVVKQSWSVMITESAKGIQTQWYSEIYTKFKEIGLGKYPFDLTATEEISIEDFELLFASGGLLDTFIQKNFAPFYDTN
LWVPKQVDGETMPLSSALLVQLRNYNVIRDTLINKSTNRVYIPFSAKVLDLDSSAIRASLKISDTDINYYHGPSRIREME
WPPQNGDFNISITIQDVTDEGKQHVLNKSGQWAIYRLLGDSTLTNTHNGSFVSDIKVSGRDLSLRITPLTQKNPFTLAEL
YNFTLPESI*					

Nucleotide sequence: 3390 bp

						>T6CP007571 NC_019955:c1486759-1483370 [Vibrio parahaemolyticus BB22OP] [TssM]
ATGTTCAAACAAAAAATACTAAGAAATCCAGTCGTGGTTTCTACCATAGTGGCTTTGGTATTAGCCGCGATATTTGCAGC
GGTATATTTATTCATATTTGAAGACGCTGATAAGCTTTATCTATGGGTTGGTTTAGGAGTTATTACTGGTTTTTATTTTA
CATTCCAGCTTTCCCTATGGCTTAAGAAAAAAAACAGCTCAAAAGTTCAACGTTTAGAGACGGAAGAAGAAGCTCTTTCA
ATGGTGATCCGCCCTCTTCTTTCTAATGCGGGCAAAAAGCCAATCTATCTGATGGTTGGTAATAAAGGGGCCGGTCGACG
TCAGTTTTTGTTTAATTCCAGCGCAATTAAGCCGATGGACCGTTCTCGCACAGCAAAAAACGATTTCTTCGAATGGTATG
AATCTGATGGTGCCGTTTATGTTAAGCCAGATCAACGCCTCATCTTCCAGGAAGTTTCAAGCAGTGATGCTTCTCTCTGG
AATACTTTTATTGATGAGGTGATTCGTCATCGTCCGAGAAAACCGTTTTCAGGTTGCTTATTCTTTGTTGATTTCGAATT
TATGATTGTATCTGAGCCAGAGCAGAAAGATTACACCATGACAGCGCTATTGCAGCGTTTGAACTCTATCACTGAGAAGA
CTTCGTCTGCACTACCTGTTTATTTGATGATGACGAAACTCGACAAACTAGATGGCTTTAAAGAATACGTTCACTCTAGT
TCACTAAAAACACGTATTGAATTTCTGTCCATTCCATTGAAGGAAGCGAAAGGTGTACTTACCGAATACTACCTAGATAG
CTTCCATAATCTGGTAAAAGTGCTAGAAAGCAACGCTTTAGACGCATCGGCAAACTCAACAGATATCAGCGAAAAGCAAG
CCATCCTTGCCTTCCCAAAACAGTTTGAACTCTGTCAACGTGAAATCAGCTACGTGCTTGAACGTCTTTGCGATACAAAC
AATGGCGTTTACTCGTTGGATATTCGCGAAATCTTCTTCTGTTCTTCTTTGCAAGGTGGACGCAAGTACAACTTGTTAGC
CAAAAGCTGCAGTAATTACTTCAATTTACCAATTATCGCATCTGAGCACACGCAACTAACTGAAACACCGTACTTTTCTC
GCTTCTTGCTTGATTCTCAAATCCTCCCAGAGAGTGACTTCGCAGGCGAGAACAAGACTTACCTACGCATGATTCAGCGT
CAAAGCCATTTGGCAATGCTGGCATCCGTTGTCTTATTGGCAGGCGGTACTTATTTTTTTGTCACAACACTAGACAGTAA
CTTGCGTGTTATTAACCAATTGCTAAATATCGACGAAAAAGTTCAAACAGAACAAGAAGCAAGCTTCAACCAGTTATTGA
TGAGTGCTACTCGTGCAATCCAACCCTCATACAATGCTTGGTTAGAAGGATCACGAGCATTAGATGAAGAAATAGTACCG
CTAAATATAAGCAGGTTAAATGAAAGTACAAAATTAGCTTATGAAGCATTGATTAAAGAGGTTGCTCAACAACTTATGCC
TGTTATTGAGCAAGGCTACCGTTTTGAACTGACACACAACCAACATGATGTCACAGCAACGTTGCCGTTATTAAAAGGCT
ACTTGATGTTGAAGGACCCGACCAAGCGAGATATTCAGTTTTTACGACGCCAAACATTGCAAACACTAACAGACTTGAGC
GATAAGCCGGATGTGGTAAATCAAGCTATGCGATACCTGGACGCCTACTTCCGCACTCAATTCTCACCTGTAGATATCAA
CATGGATGTCGTTCGAGCAACTCGTCGATCATTGTTGGCAAATTCAAATGTGGATCTTGTTTACGCAGGCATTTTAAACC
AAGCAGATGCTATCGATCTTGGCACACTCGATCTACAACGTGCAGTTGGCTTTGAATTTAACAACGTATTTAACGAGCCG
TTAGATGACAACCGACTAGTAATCGATAAGATTTATACCTCCACTGGCTTTAGTACCTTCTATCGCCCTCGCGTCGACTT
GATGTCTAAACAAGTGATCAGTGATAACTGGGTGCTAGGATTGTCCAACTATGTTACCCCAACCAAAGAAGAGCAAGAAT
CGTTTAAAAATCAAGTACGTAAAAAGTACACAGATGATTATATCAACTACTGGCGCAACGCTCTCAGTGAGTTAAAAATT
CAGAGTTATAGCAATGTGGGTGATCTAACAAATGCTATCGACCTAATCTCTGGACCATCCTCACCAATGACGACTGTATT
AAAGCAAGTCTATGCAAATACTCAGTTTTCACCGGTAGGAGAAAAAAATGTACTCATCTCGAAAGTAGACCCAAAACTGG
CAGAAGTGGCGAACTCTGCGTCAGAAGCGGTTGAAGAAGTGGTTCAGCCGGATTACTTGTTGATGAAGCGTGTAGAACAA
GCGTTCCATCTTCTAAATCAGCTTCAAGTAAGCGAGACTCCTAACTCCCCTACTCCATGGGACGAAACTATCGCAGCTTT
AAGCCGAGTTCGCACCTACATGAAAGATATTGCGGATGCTCCCGATCCACAAATGGCAGCACTTGCAGCCGCTCAACACC
GAATGAGCAGCTCTGAAGCCGATCCGCTAATTAAACTTAAACAGATTGCTCAGAAGTCACCAGAGCCAGTACGCAGTTGG
TTACTTGATGTCGTAAAGCAAAGCTGGTCGGTAATGATCACCGAATCAGCAAAAGGCATTCAAACCCAATGGTATAGTGA
AATTTACACCAAATTTAAAGAGATAGGACTTGGTAAATACCCATTTGATCTCACAGCAACAGAGGAGATTTCTATCGAAG
ACTTCGAACTGTTGTTTGCTTCAGGTGGATTGTTAGACACATTTATTCAGAAAAACTTCGCACCGTTCTACGATACCAAC
TTGTGGGTACCAAAACAGGTGGATGGTGAAACAATGCCACTATCTTCAGCCTTACTGGTGCAATTGCGCAATTACAACGT
TATCCGCGACACGCTGATCAATAAGAGTACTAACCGAGTTTATATTCCATTCAGTGCGAAAGTATTAGATCTTGACTCAA
GTGCTATCAGAGCCAGTTTGAAAATCTCTGACACTGATATCAACTACTATCACGGTCCGAGCCGAATTCGTGAAATGGAG
TGGCCACCGCAAAACGGCGACTTCAACATTAGTATCACGATCCAAGATGTGACAGACGAAGGTAAACAACACGTTTTAAA
TAAGAGTGGTCAATGGGCGATATATAGATTGCTAGGTGATTCGACCCTGACAAACACTCATAACGGTAGCTTCGTTAGTG
ACATAAAAGTATCAGGGAGGGATTTGAGCCTCCGTATAACTCCCTTGACACAAAAAAACCCTTTCACTTTGGCTGAGTTG
TACAACTTTACGCTCCCAGAGTCGATTTGA					