Detailed information of regulator
Summary
| Regulator ID | REG0329  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Transcription regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00719 (Type i1) |
| Strain | Vibrio parahaemolyticus BB22OP |
| Replicon | chromosome |
| Sequence | Protein sequence (136 a.a.); Nucleotide sequence (408 bp) |
| Description | H-NS is a negative regulator of .VgrG1 expression and secretion were activated in BB22OPΔhns and deletion of hcp1 in the Δhns background resulted in a significant loss of toxicity toward E. coli prey. |
| Reference |
External database links
| Locus tag (Gene) | VPBB_RS05425 (H-NS) |
| Coordinates (Strand) | 1170475..1170882 (-) |
| NCBI ID | 505109423 |
| RefSeq | NC_019955 |
| Uniprot ID | Q938W3_VIBPH |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| VgrG1 expression and secretion | ↑ | - |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Histone_HNS | PF00816.23 | 3.3e-19 | 33..129 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-135 | MSDLTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVEERREAEEAEREALAQQEAKLAEIAEKIAQDGIDVEALISALSGETKTKAKSKRAPRPAKYKYIDTNGQEKTWTGQGRTPSAIQEQLDAGKSLDDFLI |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9956 | - | - |
Protein sequence of regulator: 136 a.a. .
>REG0329 NC_019955:c1170882-1170475 [Vibrio parahaemolyticus BB22OP]
MSDLTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVEERREAEEAEREALAQQEAKLAEIAEKIAQDGIDVEALISALS
GETKTKAKSKRAPRPAKYKYIDTNGQEKTWTGQGRTPSAIQEQLDAGKSLDDFLI*
MSDLTKTLLNIRSLRAFSRELTLEQLEEALDKLTTVVEERREAEEAEREALAQQEAKLAEIAEKIAQDGIDVEALISALS
GETKTKAKSKRAPRPAKYKYIDTNGQEKTWTGQGRTPSAIQEQLDAGKSLDDFLI*
Nucleotide sequence of regulator: 408 bp .
>REG0329 NC_019955:c1170882-1170475 [Vibrio parahaemolyticus BB22OP]
ATGTCAGATCTGACTAAAACACTTCTAAATATCCGTAGCCTTCGTGCATTTTCTCGTGAGCTGACTCTAGAACAATTGGA
AGAAGCTCTAGATAAACTAACTACTGTTGTTGAAGAGCGTCGTGAAGCTGAAGAAGCAGAGCGTGAAGCACTAGCACAAC
AAGAAGCTAAGCTAGCAGAGATTGCAGAAAAAATTGCTCAAGACGGTATCGACGTTGAAGCACTAATTAGCGCACTATCT
GGCGAAACTAAGACTAAAGCAAAATCTAAGCGCGCTCCTCGCCCAGCTAAGTACAAGTACATCGACACAAATGGCCAAGA
AAAAACTTGGACAGGCCAAGGTCGTACACCTTCTGCAATTCAAGAGCAACTTGACGCAGGTAAATCACTAGACGATTTCC
TAATCTAA
ATGTCAGATCTGACTAAAACACTTCTAAATATCCGTAGCCTTCGTGCATTTTCTCGTGAGCTGACTCTAGAACAATTGGA
AGAAGCTCTAGATAAACTAACTACTGTTGTTGAAGAGCGTCGTGAAGCTGAAGAAGCAGAGCGTGAAGCACTAGCACAAC
AAGAAGCTAAGCTAGCAGAGATTGCAGAAAAAATTGCTCAAGACGGTATCGACGTTGAAGCACTAATTAGCGCACTATCT
GGCGAAACTAAGACTAAAGCAAAATCTAAGCGCGCTCCTCGCCCAGCTAAGTACAAGTACATCGACACAAATGGCCAAGA
AAAAACTTGGACAGGCCAAGGTCGTACACCTTCTGCAATTCAAGAGCAACTTGACGCAGGTAAATCACTAGACGATTTCC
TAATCTAA