SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01832
Effector type	Tme
Cognate immunity protein	IMU00352
Related T6SS (Type)	T6SS00719 (Type i1)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome
Sequence	Protein sequence (311 a.a.); Nucleotide sequence (933 bp)
Description	VPBB_RS15030 (Tme1) was responsible for the T6SS1-mediated toxicity, since its deletion from the T6SS1+ BB22OP attacker eliminated its ability to intoxicate a Δtme/i1 prey and Tme effectors target the periplasm of Gram-negative bacteria, causing membrane disruption and loss of membrane potential
Reference	[1] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VPBB_RS15030 (Tme1)
Coordinates (Strand)	3247654..3248586 (+)
NCBI ID	505110423
RefSeq	NC_019955
Uniprot ID	UPI0002A55042
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-310	MRYESAPVAPKERQETTRQRAARQRRERQEELRYTASDEKRWAEIRERVLRDRQEAGLGQISQGLKTPQILSDSIGLQCSLDDEMAKNHQHVLITDLEDAHKILHNVWLDSGKDVAALAASINTAKSWFNHTEPMFDAASIAKQFGDMNIKADLVESRGKIFVAFNGTDQNGKVLKHALVNGSRINMNGKKYPLNSFKAMQTGFSPKSRVANFKGAGVLTFVVSASIATSDLVFKDDYHLVDWFGNVGSDMFKFMLQSAVGEAALFAAAVLGQPIIIGAIAVTATYLLIEWAWGEYKISQTIVERLESAI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9654	-	-

Protein sequence: 311 a.a. .

                                    >EFF01832 NC_019955:3247654-3248586 Type VI Membrane-disrupting Effector (Tme) [Vibrio parahaemolyticus BB22OP]
MRYESAPVAPKERQETTRQRAARQRRERQEELRYTASDEKRWAEIRERVLRDRQEAGLGQISQGLKTPQILSDSIGLQCS
LDDEMAKNHQHVLITDLEDAHKILHNVWLDSGKDVAALAASINTAKSWFNHTEPMFDAASIAKQFGDMNIKADLVESRGK
IFVAFNGTDQNGKVLKHALVNGSRINMNGKKYPLNSFKAMQTGFSPKSRVANFKGAGVLTFVVSASIATSDLVFKDDYHL
VDWFGNVGSDMFKFMLQSAVGEAALFAAAVLGQPIIIGAIAVTATYLLIEWAWGEYKISQTIVERLESAI*

                                

Nucleotide sequence: 933 bp .

                                    >EFF01832 NC_019955:3247654-3248586 Type VI Membrane-disrupting Effector (Tme) [Vibrio parahaemolyticus BB22OP]
ATGAGATATGAATCCGCTCCTGTAGCCCCAAAAGAAAGACAAGAAACAACTAGACAGCGAGCAGCTCGCCAACGTAGAGA
GCGTCAGGAAGAGTTGAGGTATACCGCGAGCGATGAAAAGCGATGGGCAGAAATTAGAGAGAGGGTTCTTAGAGATAGAC
AAGAGGCTGGATTAGGTCAAATCTCTCAAGGTTTAAAAACGCCTCAAATTCTAAGCGATTCTATCGGGCTACAGTGTTCA
CTTGACGATGAAATGGCGAAGAATCATCAGCATGTACTTATTACTGATTTAGAAGATGCGCACAAGATATTGCATAACGT
TTGGCTAGATTCTGGTAAAGACGTTGCAGCTCTTGCAGCGAGTATTAATACAGCAAAATCATGGTTCAACCACACAGAGC
CGATGTTTGACGCCGCGTCTATTGCTAAGCAATTCGGGGATATGAACATTAAGGCGGATCTTGTTGAGAGCAGAGGAAAA
ATCTTTGTTGCATTCAATGGGACAGATCAGAACGGGAAAGTACTCAAACATGCGTTAGTGAATGGCTCTCGTATCAACAT
GAACGGAAAAAAATACCCGCTTAATTCATTTAAAGCAATGCAAACTGGTTTTAGCCCGAAATCTAGGGTTGCAAACTTTA
AAGGTGCAGGAGTTTTAACGTTTGTTGTATCGGCGAGTATTGCTACTAGTGACCTAGTGTTCAAAGATGATTACCACCTA
GTGGATTGGTTTGGGAATGTTGGCTCTGACATGTTCAAATTTATGCTCCAAAGCGCAGTTGGTGAAGCCGCATTGTTTGC
TGCTGCTGTCTTAGGTCAACCGATAATTATCGGTGCAATTGCTGTAACAGCGACATATCTTCTAATTGAATGGGCTTGGG
GCGAATACAAAATTTCTCAAACGATAGTTGAAAGGTTAGAAAGTGCGATATAA

                                