Detailed information of immunity protein


Summary

Immunity protein ID IMU00352 experimental
Cognate effector EFF01832 experimental
Related T6SS (Type) T6SS00719 experimental (Type i1)
Strain Vibrio parahaemolyticus BB22OP
Replicon chromosome
Sequence Protein sequence (143 a.a.); Nucleotide sequence (429 bp)
Description Tmi1, from a plasmid (pTmi1) was sufficient to restore immunity against T6SS1-mediated toxicity of Tme1 and the family of proteins that contain a DUF1240 or a DUF1240-like domain can be redefined as Tmi
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) VPBB_RS15035 (Tmi1)
Coordinates (Strand) 3248576..3249004 (+)
NCBI ID 491520929
RefSeq NC_019955
Uniprot ID UPI0000D53813
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 VRYKTKIR
Transmembrane helix 9-31 LAGLFFVSLMLLCWFYPSYGVYL
Outside 32-45 DYLELLNRADTVQV
Transmembrane helix 46-68 SVITLWVPVGVFGALVCVLFMTP
Inside 69-87 KALLYGKKINELYSQRAMQ
Transmembrane helix 88-110 LANKISIYFALAGVAFAAGWTYH
Outside 111-142 SIDLLEKYGYVYSRDLTKITPTGVHLMYVKAR



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9522 - -



  Protein sequence: 143 a.a.    .

>IMU00352 NC_019955:3248576-3249004 [Vibrio parahaemolyticus BB22OP]
VRYKTKIRLAGLFFVSLMLLCWFYPSYGVYLDYLELLNRADTVQVSVITLWVPVGVFGALVCVLFMTPKALLYGKKINEL
YSQRAMQLANKISIYFALAGVAFAAGWTYHSIDLLEKYGYVYSRDLTKITPTGVHLMYVKAR*

  Nucleotide sequence: 429 bp    .

>IMU00352 NC_019955:3248576-3249004 [Vibrio parahaemolyticus BB22OP]
GTGCGATATAAAACTAAAATCCGTTTGGCTGGCTTATTTTTTGTTTCACTAATGCTGTTGTGTTGGTTTTATCCTTCTTA
TGGCGTTTATTTGGATTACCTTGAGCTATTGAATCGAGCCGATACTGTGCAAGTTTCCGTTATTACCTTGTGGGTTCCTG
TTGGTGTTTTTGGGGCTTTAGTGTGTGTGCTTTTTATGACACCTAAAGCATTGCTATATGGGAAGAAGATTAATGAACTT
TATTCTCAAAGAGCTATGCAGTTAGCTAACAAAATTAGTATTTATTTTGCATTGGCAGGCGTGGCATTTGCTGCTGGCTG
GACATATCACTCTATCGACTTGTTAGAAAAATATGGGTACGTATACAGCCGAGATTTAACAAAAATCACTCCAACAGGAG
TGCACTTGATGTACGTTAAAGCTCGATGA