Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00352  |
| Cognate effector | EFF01832 |
| Related T6SS (Type) | T6SS00719 (Type i1) |
| Strain | Vibrio parahaemolyticus BB22OP |
| Replicon | chromosome |
| Sequence | Protein sequence (143 a.a.); Nucleotide sequence (429 bp) |
| Description | Tmi1, from a plasmid (pTmi1) was sufficient to restore immunity against T6SS1-mediated toxicity of Tme1 and the family of proteins that contain a DUF1240 or a DUF1240-like domain can be redefined as Tmi |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | VPBB_RS15035 (Tmi1) |
| Coordinates (Strand) | 3248576..3249004 (+) |
| NCBI ID | 491520929 |
| RefSeq | NC_019955 |
| Uniprot ID | UPI0000D53813 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-8 | VRYKTKIR |
| Transmembrane helix | 9-31 | LAGLFFVSLMLLCWFYPSYGVYL |
| Outside | 32-45 | DYLELLNRADTVQV |
| Transmembrane helix | 46-68 | SVITLWVPVGVFGALVCVLFMTP |
| Inside | 69-87 | KALLYGKKINELYSQRAMQ |
| Transmembrane helix | 88-110 | LANKISIYFALAGVAFAAGWTYH |
| Outside | 111-142 | SIDLLEKYGYVYSRDLTKITPTGVHLMYVKAR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9522 | - | - |
Protein sequence: 143 a.a. .
>IMU00352 NC_019955:3248576-3249004 [Vibrio parahaemolyticus BB22OP]
VRYKTKIRLAGLFFVSLMLLCWFYPSYGVYLDYLELLNRADTVQVSVITLWVPVGVFGALVCVLFMTPKALLYGKKINEL
YSQRAMQLANKISIYFALAGVAFAAGWTYHSIDLLEKYGYVYSRDLTKITPTGVHLMYVKAR*
VRYKTKIRLAGLFFVSLMLLCWFYPSYGVYLDYLELLNRADTVQVSVITLWVPVGVFGALVCVLFMTPKALLYGKKINEL
YSQRAMQLANKISIYFALAGVAFAAGWTYHSIDLLEKYGYVYSRDLTKITPTGVHLMYVKAR*
Nucleotide sequence: 429 bp .
>IMU00352 NC_019955:3248576-3249004 [Vibrio parahaemolyticus BB22OP]
GTGCGATATAAAACTAAAATCCGTTTGGCTGGCTTATTTTTTGTTTCACTAATGCTGTTGTGTTGGTTTTATCCTTCTTA
TGGCGTTTATTTGGATTACCTTGAGCTATTGAATCGAGCCGATACTGTGCAAGTTTCCGTTATTACCTTGTGGGTTCCTG
TTGGTGTTTTTGGGGCTTTAGTGTGTGTGCTTTTTATGACACCTAAAGCATTGCTATATGGGAAGAAGATTAATGAACTT
TATTCTCAAAGAGCTATGCAGTTAGCTAACAAAATTAGTATTTATTTTGCATTGGCAGGCGTGGCATTTGCTGCTGGCTG
GACATATCACTCTATCGACTTGTTAGAAAAATATGGGTACGTATACAGCCGAGATTTAACAAAAATCACTCCAACAGGAG
TGCACTTGATGTACGTTAAAGCTCGATGA
GTGCGATATAAAACTAAAATCCGTTTGGCTGGCTTATTTTTTGTTTCACTAATGCTGTTGTGTTGGTTTTATCCTTCTTA
TGGCGTTTATTTGGATTACCTTGAGCTATTGAATCGAGCCGATACTGTGCAAGTTTCCGTTATTACCTTGTGGGTTCCTG
TTGGTGTTTTTGGGGCTTTAGTGTGTGTGCTTTTTATGACACCTAAAGCATTGCTATATGGGAAGAAGATTAATGAACTT
TATTCTCAAAGAGCTATGCAGTTAGCTAACAAAATTAGTATTTATTTTGCATTGGCAGGCGTGGCATTTGCTGCTGGCTG
GACATATCACTCTATCGACTTGTTAGAAAAATATGGGTACGTATACAGCCGAGATTTAACAAAAATCACTCCAACAGGAG
TGCACTTGATGTACGTTAAAGCTCGATGA