SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP007566
Component protein type	TssI
T6SS ID (Type)	T6SS00719 (Type i1)
Strain	Vibrio parahaemolyticus BB22OP
Replicon	chromosome I
Sequence	Protein sequence (693 a.a.); Nucleotide sequence (2079 bp)
Reference	[1] Gode-Potratz CJ, McCarter LL. Quorum sensing and silencing in Vibrio parahaemolyticus. J Bacteriol. 2011 Aug;193(16):4224-37. doi: 10.1128/JB.00432-11. Epub 2011 Jun 24. PMID: 21705592 [2] Fridman CM, Keppel K, Gerlic M, et al. A comparative genomics methodology reveals a widespread family of membrane-disrupting T6SS effectors. Nat Commun. 2020 Feb 27;11(1):1085. doi: 10.1038/s41467-020-14951-4. PMID: 32109231

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VPBB_RS06670
Coordinate (Strand)	1467896..1469974 (+)
NCBI ID	WP_015296666.1
RefSeq	NC_019955
Uniprot ID	A0A7T4Q1F9_VIBPH
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	1.3e-77	26..331
Phage_base_V	PF04717.14	8.8e-15	387..455

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-692	MVNDVEFKFEVPGCGHEFRVESFQVNEELSKPFHISLSLLSLDPDISFDSLIRKAGTLTLYGQGLSAARIFNGVVNEVRYLGTGRRFSRYQLVLVPQAWFLSQRQDCRIFQQKSAKDIITEVLDDGSVTDYRFELSGIYPPKEYALQYRESDLHFVQRMMAEHGMWYYFDHTDSNHTMVIVDSNDAIAPLVSSPLNASYIGPIVYHADSGGVADREHISDLELVNRVRTGQVTYTDYNYEQPKIPQEMTHAGDLDQDLKQFDYPGRYVDPVMGQMRTTEWMSEHIVDNQQVEASSDVMRLASGYSFNISDHPRSEINRDYIMLSVMHTGQDPQVHEDEASGMPTTYYNQFTCIPRDVVFKAPKLAAPVVDGPQTAVVVGPAGEEIYTDKLGRIKVQFHWDRYGNNDEHASCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHFPPYSLPENKTRTTFRTQTHKGTGYNELSFEDEANQEEVYIHAQKDMSTKVLNNRYRDIGQDEFLKVARHQTNEVHGDHKETIDGHKTTQVNSTFTETVEQDVTVTYNANETQYVKNNSDLEIGDNRTTKIGKNDDLDVGENSNLTVGASKSSDIGADDNQTVGGNLTVSVKGNTSYKADGATQIISGDKIVLKTGGSSLVMNSDGSIKLSGSSITIEGSDKVVVKGGNVAIN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9826	-	-

Protein sequence: 693 a.a.

						>T6CP007566 NC_019955:1467896-1469974 [Vibrio parahaemolyticus BB22OP] [TssI]
MVNDVEFKFEVPGCGHEFRVESFQVNEELSKPFHISLSLLSLDPDISFDSLIRKAGTLTLYGQGLSAARIFNGVVNEVRY
LGTGRRFSRYQLVLVPQAWFLSQRQDCRIFQQKSAKDIITEVLDDGSVTDYRFELSGIYPPKEYALQYRESDLHFVQRMM
AEHGMWYYFDHTDSNHTMVIVDSNDAIAPLVSSPLNASYIGPIVYHADSGGVADREHISDLELVNRVRTGQVTYTDYNYE
QPKIPQEMTHAGDLDQDLKQFDYPGRYVDPVMGQMRTTEWMSEHIVDNQQVEASSDVMRLASGYSFNISDHPRSEINRDY
IMLSVMHTGQDPQVHEDEASGMPTTYYNQFTCIPRDVVFKAPKLAAPVVDGPQTAVVVGPAGEEIYTDKLGRIKVQFHWD
RYGNNDEHASCWIRVSQSMAAPTWGAVYLPRIGHEVVVTFLEGDPDRPLVTGAVYNGLHFPPYSLPENKTRTTFRTQTHK
GTGYNELSFEDEANQEEVYIHAQKDMSTKVLNNRYRDIGQDEFLKVARHQTNEVHGDHKETIDGHKTTQVNSTFTETVEQ
DVTVTYNANETQYVKNNSDLEIGDNRTTKIGKNDDLDVGENSNLTVGASKSSDIGADDNQTVGGNLTVSVKGNTSYKADG
ATQIISGDKIVLKTGGSSLVMNSDGSIKLSGSSITIEGSDKVVVKGGNVAIN*					

Nucleotide sequence: 2079 bp

						>T6CP007566 NC_019955:1467896-1469974 [Vibrio parahaemolyticus BB22OP] [TssI]
ATGGTGAATGACGTAGAATTTAAATTTGAAGTGCCAGGGTGTGGGCACGAGTTTCGAGTAGAAAGCTTTCAAGTAAATGA
AGAGCTTTCGAAACCTTTTCACATCAGTCTCTCATTACTTTCACTTGATCCAGATATCTCTTTTGATTCGTTAATACGAA
AAGCAGGTACACTGACTCTTTATGGACAAGGCTTAAGCGCGGCACGCATATTCAATGGTGTGGTGAACGAAGTTCGTTAT
TTGGGTACAGGCCGTCGCTTCTCTCGCTATCAGTTAGTTTTGGTTCCGCAAGCTTGGTTCTTATCACAACGTCAAGACTG
TCGTATTTTCCAACAAAAATCAGCGAAAGATATAATTACAGAGGTGCTTGATGATGGTTCAGTGACCGACTATCGTTTTG
AATTATCTGGTATTTATCCACCAAAAGAGTACGCGCTGCAATACCGTGAGTCAGACCTACACTTTGTTCAGCGTATGATG
GCAGAACACGGTATGTGGTACTATTTCGACCACACCGATTCAAACCACACAATGGTCATTGTCGATAGTAACGATGCCAT
TGCTCCACTGGTTAGCTCTCCTCTCAATGCATCTTATATTGGGCCGATTGTATATCACGCCGATAGTGGGGGTGTTGCTG
ACCGTGAGCATATATCCGATCTCGAGTTAGTTAACCGTGTGAGAACGGGTCAGGTTACTTACACTGACTACAATTACGAG
CAGCCAAAGATCCCGCAAGAGATGACTCACGCAGGTGATCTGGACCAAGATTTAAAGCAGTTTGATTATCCTGGACGTTA
TGTTGATCCTGTAATGGGGCAGATGAGAACCACTGAGTGGATGTCCGAGCACATTGTCGATAATCAGCAAGTAGAAGCGA
GCAGTGATGTGATGCGTTTGGCGTCTGGTTACAGCTTTAACATCAGTGACCATCCTCGCTCTGAAATCAACCGTGATTAC
ATCATGTTATCCGTCATGCACACAGGGCAAGACCCACAAGTGCACGAAGACGAAGCGAGTGGCATGCCAACTACTTACTA
CAACCAGTTCACGTGTATTCCACGTGATGTTGTATTCAAAGCGCCCAAGTTGGCGGCTCCAGTAGTGGATGGTCCGCAAA
CTGCGGTTGTAGTTGGTCCTGCAGGGGAGGAGATTTACACCGATAAACTAGGACGAATTAAGGTTCAGTTCCATTGGGAT
CGCTATGGTAACAACGATGAACATGCAAGTTGCTGGATTCGTGTGAGTCAATCAATGGCTGCTCCAACGTGGGGCGCGGT
TTACTTGCCACGTATTGGTCATGAAGTCGTAGTCACCTTCCTTGAAGGCGACCCAGATCGCCCATTGGTGACTGGCGCAG
TTTATAACGGTCTTCACTTCCCTCCGTACTCGTTGCCTGAGAACAAAACTCGTACTACGTTCCGTACGCAAACGCACAAA
GGCACCGGCTACAACGAACTGAGTTTTGAAGACGAAGCGAACCAAGAAGAAGTCTACATTCATGCTCAAAAAGACATGTC
GACCAAAGTTCTCAACAATCGTTACCGAGATATCGGTCAGGATGAGTTCTTAAAGGTTGCGCGTCATCAGACGAACGAAG
TCCATGGTGATCATAAAGAAACCATTGATGGCCACAAAACAACCCAAGTAAACAGCACTTTTACCGAGACAGTAGAGCAA
GACGTTACCGTTACATACAACGCCAACGAAACCCAATATGTGAAGAACAATTCTGATTTGGAAATTGGTGATAACCGCAC
AACCAAGATCGGCAAGAACGATGACTTGGATGTGGGTGAAAACAGTAACTTAACTGTTGGTGCATCAAAAAGCTCGGACA
TTGGTGCCGATGATAACCAAACAGTGGGTGGCAACTTAACCGTATCGGTAAAAGGCAATACATCTTACAAAGCCGATGGC
GCGACACAGATCATCAGTGGAGACAAGATTGTACTGAAAACAGGCGGTTCTTCTTTAGTGATGAACAGTGATGGTTCAAT
CAAGCTCTCAGGGTCTTCAATCACCATTGAAGGCAGCGATAAAGTCGTCGTGAAAGGTGGTAACGTAGCGATTAACTAA					