Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00274  |
| T6SS type | Type i4a |
| Strain | Paraburkholderia phymatum STM815 |
| Replicon (RefSeq) | plasmid pBPHY01 (NC_010625) |
| Location | 510771..535444 |
| Function | anti-bacterial |
| Comment | Both T6SSs of P. phymatum play an important role in the competition against other bacteria. T6SS-b is highly expressed in presence of Citrate. The presence of a germinated seed induced T6SS-b expression. T6SS-b is involved in P. phymatum motility. |
| References | [1] de Campos SB, Lardi M, Gandolfi A, Eberl L, Pessi G. et al (2017) Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition.. Front Microbiol. 2.050694444. [PudMed:29312183] [2] Hug S, Liu Y, Heiniger B, Bailly A, Ahrens CH, Eberl L, Pessi G. et al (2021) Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction.. Front Plant Sci. 12:699590. [PudMed:34394152] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BPHY_RS29870 | 506119..507693 | + | 1575 | WP_012405198.1 | hybrid sensor histidine kinase/response regulator | ||
| BPHY_RS29875 | 507744..509531 | - | 1788 | WP_012405199.1 | hypothetical protein | ||
| T6CP002738 | TssB | BPHY_RS29885 (tssB) | 510771..511343 | + | 573 | WP_012405201.1 | type VI secretion system contractile sheath small subunit |
| T6CP002739 | TssC | BPHY_RS29890 (tssC) | 511410..512918 | + | 1509 | WP_012405202.1 | type VI secretion system contractile sheath large subunit |
| T6CP002740 | TssD | BPHY_RS29895 | 512987..513475 | + | 489 | WP_012405203.1 | type VI secretion system tube protein Hcp |
| T6CP002741 | TssE | BPHY_RS29900 (tssE) | 513551..514006 | + | 456 | WP_012405204.1 | type VI secretion system baseplate subunit TssE |
| T6CP002742 | TssF | BPHY_RS29905 (tssF) | 514008..515765 | + | 1758 | WP_041765651.1 | type VI secretion system baseplate subunit TssF |
| T6CP002743 | TssG | BPHY_RS29910 (tssG) | 515720..516853 | + | 1134 | WP_012405206.1 | type VI secretion system baseplate subunit TssG |
| T6CP002744 | TssH | BPHY_RS29915 (tssH) | 516865..519594 | + | 2730 | WP_012405207.1 | type VI secretion system ATPase TssH |
| T6CP002745 | TssI | BPHY_RS29920 (tssI) | 519599..521524 | + | 1926 | WP_012405208.1 | type VI secretion system tip protein VgrG |
| BPHY_RS29925 | 521533..522255 | + | 723 | WP_012405209.1 | toxin-antitoxin system YwqK family antitoxin | ||
| BPHY_RS29930 | 522278..522664 | + | 387 | WP_012405210.1 | DUF4280 domain-containing protein | ||
| BPHY_RS29935 | 522678..522953 | + | 276 | WP_041765654.1 | hypothetical protein | ||
| BPHY_RS29940 | 522967..523941 | + | 975 | WP_012405212.1 | ABC transporter substrate-binding protein | ||
| BPHY_RS29945 | 523973..525247 | + | 1275 | WP_012405213.1 | cation:dicarboxylase symporter family transporter | ||
| BPHY_RS29950 | 525256..525957 | + | 702 | WP_012405214.1 | amino acid racemase | ||
| BPHY_RS29955 | 525954..527378 | + | 1425 | WP_012405215.1 | cation:dicarboxylase symporter family transporter | ||
| BPHY_RS29960 | 527495..527887 | + | 393 | WP_157686916.1 | hypothetical protein | ||
| T6CP002746 | TssK | BPHY_RS29965 (tssK) | 527884..529218 | + | 1335 | WP_012405217.1 | type VI secretion system baseplate subunit TssK |
| T6CP002747 | TssL | BPHY_RS29970 | 529280..530032 | + | 753 | WP_012405218.1 | DotU family type IV/VI secretion system protein |
| T6CP002748 | TssM | BPHY_RS29975 | 530121..534299 | + | 4179 | WP_012405219.1 | hypothetical protein |
| T6CP002749 | TssA | BPHY_RS29980 (tssA) | 534296..535444 | + | 1149 | WP_012405220.1 | type VI secretion system protein TssA |
| BPHY_RS29985 | 535602..536714 | + | 1113 | WP_012405221.1 | hypothetical protein | ||
| BPHY_RS29990 | 537050..537469 | + | 420 | WP_157686866.1 | helix-turn-helix transcriptional regulator | ||
| BPHY_RS42080 | 537603..537800 | - | 198 | WP_041765655.1 | hypothetical protein | ||
| BPHY_RS30000 | 538809..539006 | - | 198 | WP_012405225.1 | hypothetical protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
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Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
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