Detailed information of component protein


Summary

Component ID T6CP002745
Component protein type TssI
T6SS ID (Type) T6SS00274 experimental (Type i4a)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (642 a.a.); Nucleotide sequence (1926 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS29920
Coordinate (Strand) 519599..521524 (+)
NCBI ID WP_012405208.1
RefSeq NC_010625
Uniprot ID B2JVS1_PARP8
KEGG ID bph:Bphy_5985
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 9.2e-82 31..328
Phage_base_V PF04717.14 9.3e-17 374..441
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-641 MSTTPSQANCFASVSTPFGTDVLLLDGFGGREAIAELFHFDLRMRSTNKALDPQQIVGKSATVTLKDHTGVARYFNGIVTRFAHAGADVQYGFYSAELAPRLWLLSLGQDRVIWQNLSALEIIKKVLGTFGVTFEDRTKHSSNYLAREYCVQYDESAFRFISRLMEEEGIFYFFTFADGAHTMVLADDPSAHEATVAGTLYFAPDPSVGQQIQRLSAFEMSRGVVAGEHIVSDYDYTQAATLLSSSKGASSIPTGTRFTFPGKYVAASHGDQIAGTRLQAHHVAQQTGRGEGAYYGLAAGTTFTLSGHPDSALNVSYVVRAVQHAASNMTYGNEFDVIPVTVPFRAPLVTPRPIVSGTHTAKVVGPSDEEIWTDSQGRIKLKFYWDRTPDADQNSSCWVRVAQASAGPGWGHLFLPRIGEEVVVSYVDGDPDRPLVTGCVYNGTNAVPVTLPSMQTQSVIRSRSSKGGTAGNEIRMEDKLNSEELYVHAQKDMSVEIENALSTTVKAGAETHVVLKGDRSVEVSEGKETHTVKGTRTLDVTGDETHTNHAAFAHNVSGNHTHKIDGNYTLKVGGNLVIEVSGSISIKAGTSLSSEAGTSHASKAGTTLSSEGMTVSHKASATQTVDGGGQLALKGGIVQLN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9594 - -


  Protein sequence: 642 a.a.    

>T6CP002745 NC_010625:519599-521524 [Paraburkholderia phymatum STM815] [TssI]
MSTTPSQANCFASVSTPFGTDVLLLDGFGGREAIAELFHFDLRMRSTNKALDPQQIVGKSATVTLKDHTGVARYFNGIVT
RFAHAGADVQYGFYSAELAPRLWLLSLGQDRVIWQNLSALEIIKKVLGTFGVTFEDRTKHSSNYLAREYCVQYDESAFRF
ISRLMEEEGIFYFFTFADGAHTMVLADDPSAHEATVAGTLYFAPDPSVGQQIQRLSAFEMSRGVVAGEHIVSDYDYTQAA
TLLSSSKGASSIPTGTRFTFPGKYVAASHGDQIAGTRLQAHHVAQQTGRGEGAYYGLAAGTTFTLSGHPDSALNVSYVVR
AVQHAASNMTYGNEFDVIPVTVPFRAPLVTPRPIVSGTHTAKVVGPSDEEIWTDSQGRIKLKFYWDRTPDADQNSSCWVR
VAQASAGPGWGHLFLPRIGEEVVVSYVDGDPDRPLVTGCVYNGTNAVPVTLPSMQTQSVIRSRSSKGGTAGNEIRMEDKL
NSEELYVHAQKDMSVEIENALSTTVKAGAETHVVLKGDRSVEVSEGKETHTVKGTRTLDVTGDETHTNHAAFAHNVSGNH
THKIDGNYTLKVGGNLVIEVSGSISIKAGTSLSSEAGTSHASKAGTTLSSEGMTVSHKASATQTVDGGGQLALKGGIVQL
N*

  Nucleotide sequence: 1926 bp    

>T6CP002745 NC_010625:519599-521524 [Paraburkholderia phymatum STM815] [TssI]
ATGTCCACAACGCCCAGCCAGGCGAACTGCTTTGCCTCCGTCTCGACACCGTTCGGAACGGATGTGCTGCTGCTCGACGG
ATTCGGAGGCCGCGAAGCGATAGCGGAGTTGTTTCATTTCGATCTGCGCATGCGCTCGACGAACAAGGCACTCGACCCTC
AGCAAATCGTCGGCAAGAGCGCAACGGTGACGCTGAAGGACCACACAGGCGTCGCCCGCTATTTCAATGGAATCGTCACA
CGCTTCGCGCACGCGGGCGCGGACGTGCAATACGGGTTCTATTCTGCTGAACTGGCTCCGCGTCTGTGGCTGCTCTCGCT
GGGTCAGGACCGTGTCATCTGGCAGAACCTGAGCGCACTCGAGATCATCAAGAAGGTGCTGGGTACGTTCGGCGTGACTT
TCGAGGACCGCACGAAGCACAGCAGCAACTACCTCGCGCGCGAATACTGCGTCCAGTACGACGAAAGTGCGTTCCGGTTC
ATCTCGCGCCTGATGGAAGAAGAGGGCATCTTCTACTTCTTCACATTCGCGGACGGCGCGCACACGATGGTGCTGGCGGA
CGATCCATCGGCGCACGAAGCCACGGTGGCAGGAACACTCTATTTCGCGCCCGATCCGAGCGTCGGCCAGCAGATCCAGC
GCCTCAGCGCGTTTGAAATGTCGCGCGGCGTGGTGGCGGGCGAGCACATTGTCAGCGACTACGACTACACGCAGGCGGCC
ACGTTGCTTTCGTCGTCGAAGGGTGCTTCGAGCATTCCGACGGGGACGCGTTTCACCTTCCCCGGCAAATATGTCGCGGC
TTCGCACGGCGACCAGATCGCCGGCACACGTCTCCAGGCACATCATGTCGCGCAGCAGACGGGACGGGGCGAGGGCGCAT
ATTACGGCCTCGCGGCAGGCACGACGTTCACGTTGAGCGGTCATCCTGACAGCGCACTCAATGTGAGCTACGTCGTGCGC
GCCGTGCAGCACGCGGCATCGAACATGACGTATGGCAACGAATTCGACGTCATCCCCGTCACCGTGCCGTTTCGCGCTCC
CCTTGTTACGCCACGGCCCATCGTATCGGGTACGCATACGGCAAAAGTGGTGGGGCCGTCGGACGAAGAGATCTGGACCG
ACTCGCAAGGGCGCATCAAGCTCAAGTTCTACTGGGACCGCACGCCTGATGCCGACCAGAACAGCTCCTGCTGGGTGCGC
GTGGCACAGGCATCGGCGGGGCCGGGGTGGGGGCATCTGTTTCTGCCGCGCATCGGCGAGGAAGTGGTGGTCAGCTATGT
CGACGGCGATCCCGACCGCCCGCTTGTGACGGGTTGTGTCTACAACGGCACGAACGCTGTGCCGGTGACTTTGCCGTCGA
TGCAAACGCAAAGCGTGATCCGCTCCCGTTCGTCGAAAGGCGGCACGGCAGGCAACGAAATCCGCATGGAGGACAAGCTC
AATTCCGAGGAGCTGTATGTCCATGCGCAGAAGGACATGAGCGTCGAGATCGAAAACGCGCTCTCCACCACCGTCAAGGC
GGGCGCGGAAACGCACGTGGTGCTGAAAGGCGATCGCAGCGTCGAGGTGAGCGAGGGCAAGGAGACGCACACCGTCAAAG
GCACACGTACGCTCGACGTGACAGGCGATGAAACGCATACCAACCATGCGGCGTTTGCGCACAACGTCAGCGGCAATCAC
ACCCACAAGATCGACGGCAATTACACATTGAAGGTAGGCGGCAATCTGGTGATCGAAGTCAGTGGCTCGATCAGCATCAA
GGCGGGCACGTCGTTGTCCAGCGAAGCGGGCACGTCGCACGCGAGCAAGGCGGGGACGACGCTCAGCAGCGAAGGCATGA
CAGTTTCGCATAAGGCCTCGGCCACGCAGACTGTCGATGGCGGCGGTCAGCTCGCCCTCAAGGGCGGCATCGTCCAGCTC
AACTGA