SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002738
Component protein type	TssB
T6SS ID (Type)	T6SS00274 (Type i4a)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (191 a.a.); Nucleotide sequence (573 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS29885
Coordinate (Strand)	510771..511343 (+)
NCBI ID	WP_012405201.1
RefSeq	NC_010625
Uniprot ID	B2JVR4_PARP8
KEGG ID	bph:Bphy_5978
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	2e-56	11..160

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-190	MSNSLQKWVGRNRPPRVQITYDVEVGDAVEKRELPLVVGLLADLSGQPATPLPKLKERRLVEIDRDNFDEIMGKIAPRLDLSVPDTLKGEGNIKVELKFEKFQDFHPESIVNQVPRLARLLEARQQLRDLLAKLDGNDELDSMLERIVQNSEELKKVQSQAHASDAPVAASAASDAVAEPAAAPDDAPAA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9952	-	-

Protein sequence: 191 a.a.

						>T6CP002738 NC_010625:510771-511343 [Paraburkholderia phymatum STM815] [TssB]
MSNSLQKWVGRNRPPRVQITYDVEVGDAVEKRELPLVVGLLADLSGQPATPLPKLKERRLVEIDRDNFDEIMGKIAPRLD
LSVPDTLKGEGNIKVELKFEKFQDFHPESIVNQVPRLARLLEARQQLRDLLAKLDGNDELDSMLERIVQNSEELKKVQSQ
AHASDAPVAASAASDAVAEPAAAPDDAPAA*					

Nucleotide sequence: 573 bp

						>T6CP002738 NC_010625:510771-511343 [Paraburkholderia phymatum STM815] [TssB]
ATGTCGAATAGTTTGCAGAAGTGGGTGGGACGCAATCGTCCGCCGCGTGTCCAGATCACCTACGACGTAGAAGTCGGCGA
CGCGGTTGAGAAGCGCGAGCTTCCGCTAGTGGTCGGTTTGCTGGCGGACCTCTCGGGACAGCCTGCAACGCCGCTCCCCA
AGCTCAAGGAACGTCGTCTGGTCGAGATCGACCGTGACAACTTCGATGAGATCATGGGCAAGATCGCCCCGCGTCTCGAC
CTCTCCGTGCCGGACACGCTCAAGGGCGAGGGCAACATCAAGGTCGAACTGAAGTTCGAGAAGTTCCAGGATTTCCATCC
CGAAAGCATCGTCAACCAGGTGCCGCGCCTCGCCAGGCTGCTCGAAGCGCGTCAGCAGCTGCGCGATCTGCTCGCCAAGC
TCGACGGTAACGACGAACTCGATTCGATGCTCGAGCGTATCGTCCAGAACAGCGAGGAACTGAAGAAAGTTCAAAGTCAG
GCGCATGCCAGCGATGCTCCCGTGGCTGCATCGGCGGCATCCGACGCAGTCGCAGAGCCGGCTGCTGCCCCGGACGACGC
ACCTGCCGCCTGA					