SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002748
Component protein type	TssM
T6SS ID (Type)	T6SS00274 (Type i4a)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (1393 a.a.); Nucleotide sequence (4179 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS29975
Coordinate (Strand)	530121..534299 (+)
NCBI ID	WP_012405219.1
RefSeq	NC_010625
Uniprot ID	B2JVT2_PARP8
KEGG ID	bph:Bphy_5996
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	2.2e-61	139..430

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MLTSNL
Transmembrane helix	7-29	FLLSIALLTLVVCAVLGAVLYFA
Outside	30-1392	IHGSHAKPQTERKIVRLRSDSLRSAFRQAVELIEGNIASRAERYNIPWIMILNEGDDPRPLPIAQAGVASVLGAEAASSAATQGISWHFFDRGIVVDIKAAYLGSPDDDGDGKPWDEFLGLCRNYRQQRPFDSVVITVPAATLLADNTDARLELVRHAKLAHRRLWLAQNRFAMRFAVYVVITGCEALPGFPTFARALPEPMRASMLGWSSPYDLSTTYQPDWVDTAMNSVMRSVSDVSAELFALDSAHLDARQFLQLPSRIDAMRAQLQLYVDELLRASAYHEPFFFRGIYLTGDASEFAQWSAAQGGEAAQPGALPSPADARRDSVMPYAGASGTEEHTDLALGDGGREPGGAINDLMLQPAFLRDLFEKKIFLEYGLTRPSRSQHLTRPVMSRALRWGSIALLGGWGMGLVVATVQLSHRNGELVTALGSLRRDTGERAAALQQGQDLPADWYRRKALALIVLNQHLRTDSTWTVFMPGSWSIVDDLNARVKERFEREFGEIAVAALEREMYARVSQLTGVGRDPSTGQLIAGDDCAAPGGMRADAGAAPSLAVDDLPEMRSLQLYVNNVDRFDAALQALQRLQHPAADNADALRLVVRYALGAELQGSVSGSLPYFYREQSGVAARTSGIDLVPVQQALRCTLNKGAQQLDNGLFTNNPLLVAERTVTDNLNTLSVSDSGTGDFSKLTAGYRAIVAGIDAQRDLLASGKGSWLHQPQFMPGPAYDHTLSRVAQNRLLGADLAAQIRQRDDTEFQAFRSELALRFGGATSGIVWADKDARYSVSPERTALRDGLSNLLNQPFMVAPRNRELPTLADGSTIAWDRAQLDQALALGDVRKHFLTDGMTNMPAAIRPMIEDALDLQFARLILDQTAAAATVTAAQGDPDSAAFDAARVRLTRIEALLMELGATAQLEELGMLVSADAIAHLQRVDNALTQSELYATRQDGGNEGQHGGRSPVLAAFGVADGAGLAAYLDQQAARAVSLGKQASVYLSALDAASATAPLVQRWQAINRDLERYQLKNPNSSLLRLEQFALSVAADPGPAGCMSKFTARPATNGDGDYFAMLHARLYDRLLARCSQSYVSDLRQRWASFASEFNETVAGRQPFDNSNMVRVGTRADVGSADFGELGQVLKRYAPVSEAFHAARGSAIAQAGAAGVAGGKLRQFIDNFDQVRTLLAPLYPSDDGAPNGYELSVEFRANRVGEIAANQVIDWTLTIGSQSLSMNEAPRTLHWDYGTPVSLALRFAKDSPLTAMSDPQQRALSTDGRTLTWQFTDPWALISFINRQRVTDASPRADNAAQLLKFDFPLSTVDPANLSLLPKQAHGRVFVRLALMPAGKKTPLPWPGSFPVRAPDWTAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9650	-	-

Protein sequence: 1393 a.a.

						>T6CP002748 NC_010625:530121-534299 [Paraburkholderia phymatum STM815] [TssM]
MLTSNLFLLSIALLTLVVCAVLGAVLYFAIHGSHAKPQTERKIVRLRSDSLRSAFRQAVELIEGNIASRAERYNIPWIMI
LNEGDDPRPLPIAQAGVASVLGAEAASSAATQGISWHFFDRGIVVDIKAAYLGSPDDDGDGKPWDEFLGLCRNYRQQRPF
DSVVITVPAATLLADNTDARLELVRHAKLAHRRLWLAQNRFAMRFAVYVVITGCEALPGFPTFARALPEPMRASMLGWSS
PYDLSTTYQPDWVDTAMNSVMRSVSDVSAELFALDSAHLDARQFLQLPSRIDAMRAQLQLYVDELLRASAYHEPFFFRGI
YLTGDASEFAQWSAAQGGEAAQPGALPSPADARRDSVMPYAGASGTEEHTDLALGDGGREPGGAINDLMLQPAFLRDLFE
KKIFLEYGLTRPSRSQHLTRPVMSRALRWGSIALLGGWGMGLVVATVQLSHRNGELVTALGSLRRDTGERAAALQQGQDL
PADWYRRKALALIVLNQHLRTDSTWTVFMPGSWSIVDDLNARVKERFEREFGEIAVAALEREMYARVSQLTGVGRDPSTG
QLIAGDDCAAPGGMRADAGAAPSLAVDDLPEMRSLQLYVNNVDRFDAALQALQRLQHPAADNADALRLVVRYALGAELQG
SVSGSLPYFYREQSGVAARTSGIDLVPVQQALRCTLNKGAQQLDNGLFTNNPLLVAERTVTDNLNTLSVSDSGTGDFSKL
TAGYRAIVAGIDAQRDLLASGKGSWLHQPQFMPGPAYDHTLSRVAQNRLLGADLAAQIRQRDDTEFQAFRSELALRFGGA
TSGIVWADKDARYSVSPERTALRDGLSNLLNQPFMVAPRNRELPTLADGSTIAWDRAQLDQALALGDVRKHFLTDGMTNM
PAAIRPMIEDALDLQFARLILDQTAAAATVTAAQGDPDSAAFDAARVRLTRIEALLMELGATAQLEELGMLVSADAIAHL
QRVDNALTQSELYATRQDGGNEGQHGGRSPVLAAFGVADGAGLAAYLDQQAARAVSLGKQASVYLSALDAASATAPLVQR
WQAINRDLERYQLKNPNSSLLRLEQFALSVAADPGPAGCMSKFTARPATNGDGDYFAMLHARLYDRLLARCSQSYVSDLR
QRWASFASEFNETVAGRQPFDNSNMVRVGTRADVGSADFGELGQVLKRYAPVSEAFHAARGSAIAQAGAAGVAGGKLRQF
IDNFDQVRTLLAPLYPSDDGAPNGYELSVEFRANRVGEIAANQVIDWTLTIGSQSLSMNEAPRTLHWDYGTPVSLALRFA
KDSPLTAMSDPQQRALSTDGRTLTWQFTDPWALISFINRQRVTDASPRADNAAQLLKFDFPLSTVDPANLSLLPKQAHGR
VFVRLALMPAGKKTPLPWPGSFPVRAPDWTAL*					

Nucleotide sequence: 4179 bp

						>T6CP002748 NC_010625:530121-534299 [Paraburkholderia phymatum STM815] [TssM]
ATGCTGACGAGCAATCTGTTTCTGCTTTCCATTGCGCTGCTGACGCTGGTGGTCTGCGCCGTGCTGGGTGCCGTGCTGTA
TTTCGCGATCCACGGTTCGCACGCGAAGCCGCAAACCGAGCGCAAGATCGTGCGTTTGCGCTCGGACTCGTTGCGTAGCG
CTTTTCGCCAGGCCGTCGAGCTGATCGAAGGCAATATCGCGTCGCGCGCCGAGCGCTACAACATTCCGTGGATCATGATC
CTCAATGAAGGGGACGACCCGCGTCCGCTGCCGATTGCTCAGGCGGGCGTCGCCAGCGTGCTGGGTGCCGAGGCGGCGAG
CTCGGCCGCCACGCAAGGCATCTCATGGCACTTCTTCGATCGGGGGATTGTGGTCGATATCAAGGCGGCCTATCTCGGTT
CGCCCGATGACGATGGCGACGGCAAGCCGTGGGACGAGTTTCTCGGTTTATGTCGCAACTACCGCCAGCAGCGCCCGTTC
GATTCCGTCGTGATCACGGTGCCGGCTGCCACGCTGCTCGCCGATAACACCGACGCGCGCCTTGAACTCGTGCGTCACGC
GAAGCTCGCGCACCGCCGGTTGTGGCTTGCGCAGAACCGTTTCGCAATGCGCTTTGCTGTGTACGTGGTCATCACCGGCT
GCGAAGCGTTGCCCGGCTTCCCGACCTTCGCGCGCGCGCTCCCTGAGCCGATGCGCGCCAGCATGCTGGGCTGGTCGTCG
CCGTACGATCTTTCGACGACCTATCAGCCCGACTGGGTCGACACGGCGATGAACAGCGTGATGCGCTCCGTGTCCGACGT
AAGCGCCGAACTCTTCGCACTCGATTCGGCGCATCTCGACGCGCGGCAGTTCCTGCAATTGCCCTCCCGCATCGACGCGA
TGCGCGCGCAACTGCAGCTCTATGTGGATGAGCTGCTGCGCGCGAGCGCATACCACGAACCGTTCTTTTTTCGCGGCATC
TACCTGACGGGGGACGCAAGCGAATTCGCGCAATGGAGCGCCGCGCAGGGCGGCGAAGCGGCGCAACCCGGCGCGTTGCC
ATCACCCGCCGATGCCCGGCGCGACAGCGTGATGCCTTATGCGGGCGCCAGCGGAACCGAAGAACATACCGATCTCGCCC
TCGGCGATGGCGGGAGGGAGCCGGGCGGCGCGATCAACGACCTGATGTTGCAGCCGGCATTCCTGCGCGATCTCTTCGAG
AAGAAAATCTTCCTTGAATACGGCCTGACGCGTCCATCGCGTTCGCAGCATCTGACGCGTCCCGTGATGAGCCGCGCGCT
GCGCTGGGGCAGCATCGCGCTGCTGGGCGGCTGGGGCATGGGTCTCGTCGTCGCAACCGTGCAACTGAGCCATCGCAATG
GCGAGCTTGTCACCGCGCTCGGCTCGCTGCGCCGCGATACCGGCGAGCGCGCTGCGGCCTTGCAGCAAGGGCAGGATTTG
CCCGCTGACTGGTATCGCCGCAAGGCGCTCGCGCTCATCGTACTGAACCAGCATCTGAGAACTGACAGCACGTGGACGGT
TTTCATGCCGGGCTCGTGGAGCATTGTCGATGACCTCAATGCACGCGTGAAAGAGCGTTTCGAGCGCGAGTTCGGCGAAA
TCGCTGTTGCGGCGCTCGAACGCGAAATGTACGCACGGGTTAGTCAGCTGACGGGAGTGGGGCGCGATCCGAGCACCGGC
CAGCTGATTGCGGGCGATGACTGTGCGGCGCCGGGCGGCATGCGCGCCGATGCTGGCGCTGCGCCGAGTCTCGCCGTCGA
CGATCTTCCCGAGATGCGTTCGCTGCAGCTCTACGTGAACAACGTCGATCGATTCGATGCCGCGTTGCAGGCGCTGCAGA
GGCTCCAGCACCCGGCGGCAGACAACGCCGATGCATTGCGCCTCGTGGTGCGTTACGCGCTCGGCGCGGAATTGCAGGGA
AGCGTGTCTGGAAGCCTGCCTTACTTCTATCGCGAGCAAAGCGGTGTGGCGGCGCGCACGTCGGGCATCGATCTTGTACC
TGTGCAGCAGGCGTTGCGCTGTACGCTGAACAAGGGCGCTCAGCAACTCGACAACGGACTCTTCACGAACAACCCGCTGC
TGGTTGCCGAGCGCACGGTGACCGATAACCTCAACACGCTTTCGGTGTCGGACTCGGGTACTGGCGACTTTTCGAAGCTC
ACCGCGGGCTATCGCGCGATCGTGGCGGGTATCGATGCACAGCGCGATTTGCTCGCATCGGGCAAGGGCAGCTGGCTCCA
TCAGCCGCAATTCATGCCGGGGCCCGCGTATGACCACACGCTTTCGCGCGTGGCTCAAAACCGCCTGCTTGGCGCAGATC
TCGCGGCACAGATCCGGCAGCGGGACGACACGGAATTTCAGGCGTTTCGCAGCGAACTGGCGCTGCGTTTCGGTGGGGCC
ACCAGCGGCATCGTCTGGGCCGACAAGGACGCGCGCTATAGCGTTTCGCCCGAGCGCACTGCGTTGCGCGATGGACTGTC
GAATCTGCTGAACCAACCGTTCATGGTGGCGCCGCGCAATCGTGAACTGCCGACGCTCGCCGATGGTTCGACGATCGCCT
GGGATCGTGCGCAACTCGACCAGGCGCTCGCGCTCGGCGACGTGCGCAAGCACTTCCTGACGGATGGCATGACTAACATG
CCTGCCGCGATACGTCCCATGATCGAAGATGCGCTCGATCTCCAGTTTGCGCGCCTGATCCTCGATCAGACAGCGGCGGC
GGCAACCGTGACGGCGGCGCAAGGCGATCCGGACAGCGCGGCGTTCGACGCGGCACGCGTGCGGCTCACGCGAATCGAGG
CGCTTCTGATGGAGCTGGGCGCGACCGCGCAACTCGAAGAACTCGGAATGCTCGTGTCGGCGGATGCAATCGCGCATTTG
CAGCGGGTGGACAACGCGCTCACGCAGTCCGAACTCTATGCGACGCGCCAGGACGGCGGCAACGAGGGCCAGCATGGCGG
ACGCTCGCCCGTGCTCGCGGCATTTGGAGTTGCGGATGGGGCCGGGCTCGCGGCGTATCTGGATCAGCAGGCAGCGCGCG
CGGTGTCGCTCGGAAAACAGGCTTCCGTGTATCTGTCGGCACTTGACGCTGCCAGCGCAACAGCGCCCCTTGTGCAGCGC
TGGCAGGCGATCAACCGCGATCTCGAACGCTATCAGCTCAAGAATCCGAATAGCAGTCTGCTGCGCCTCGAGCAGTTCGC
GCTATCAGTAGCGGCCGATCCGGGACCGGCGGGCTGCATGTCGAAGTTCACGGCTCGACCGGCTACCAATGGCGACGGCG
ACTACTTCGCGATGCTGCACGCCCGGCTCTATGACCGGCTGCTCGCGCGTTGCAGTCAGTCTTACGTATCCGATTTGCGG
CAGCGATGGGCGAGTTTCGCCAGCGAGTTCAACGAAACCGTGGCAGGGCGTCAGCCGTTCGACAACAGCAACATGGTGCG
TGTCGGCACGCGCGCCGACGTGGGCTCGGCTGACTTCGGCGAACTCGGTCAGGTGCTCAAGCGCTACGCGCCTGTATCGG
AGGCATTCCACGCAGCGCGCGGCAGCGCCATCGCGCAGGCGGGCGCGGCCGGTGTTGCGGGCGGCAAGCTGCGCCAGTTC
ATCGATAACTTCGATCAGGTCAGAACGCTGCTCGCGCCGTTGTACCCGTCTGATGACGGCGCGCCGAACGGCTACGAGCT
GAGTGTCGAGTTTCGCGCTAACCGCGTGGGCGAGATAGCGGCCAATCAGGTGATCGACTGGACGCTGACGATCGGCTCGC
AGAGCCTGTCGATGAACGAAGCGCCGCGCACGCTGCACTGGGATTACGGCACGCCCGTCTCGCTCGCATTGCGATTCGCG
AAAGACTCGCCACTCACAGCAATGTCCGACCCGCAGCAGCGCGCACTCTCGACGGACGGCCGCACGCTCACATGGCAGTT
CACAGACCCGTGGGCGTTGATCTCGTTCATCAATCGCCAGCGTGTGACTGACGCGAGCCCGCGTGCAGACAATGCGGCGC
AGTTGCTGAAGTTCGACTTCCCGCTTTCTACCGTCGATCCCGCGAATCTCAGCCTGTTGCCCAAGCAGGCGCACGGACGC
GTGTTCGTGCGCCTGGCACTCATGCCGGCGGGCAAGAAAACACCGCTGCCGTGGCCCGGCAGCTTCCCGGTGCGCGCACC
GGACTGGACCGCGCTATGA					