Detailed information of component protein


Summary

Component ID T6CP002746
Component protein type TssK
T6SS ID (Type) T6SS00274 experimental (Type i4a)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (445 a.a.); Nucleotide sequence (1335 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS29965
Coordinate (Strand) 527884..529218 (+)
NCBI ID WP_012405217.1
RefSeq NC_010625
Uniprot ID B2JVT0_PARP8
KEGG ID bph:Bphy_5994
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 1.6e-37 25..321
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-444 MMHDANMAVTDRIEWFEGMLLSPQHFQQMASRVDSLVAWQTLAAAPFSWGVRRLVLDHGLLPAGLLRVLELDAIMPDGTAVTYSAEAQGAVRLELSLEPFAASLTSGPIDFYLVLPVAGTMRRSAQVKRFRSSAGMPVEDQVSDAPPADIPRLMPNLALAAGELPSAMHVYLRLGSLYRDNEVVRLGEHLPPLLEVARDNPLWTSVASLLGQLRGKAAFVARQTANPSSKVDDRLTQLELKDRLRSLLSALPLAEAVLRTPHLHPLSLYLSLASLNGSLAMLKPGGLPPVPPDYDHADPLSVFTPLLRSLRESMSEVNEAYREHKFEFRHGAFEIALHPDWIGERLVVGLRGQSDRDLLAWMDGAVIGSQSVYASLRSRRVLGAARRGIDYAEDLGLRSGSGYLLFEVEAEHALVLASELLVIGNPNEGASAQRPQEMLLFVKG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9913 - -


  Protein sequence: 445 a.a.    

>T6CP002746 NC_010625:527884-529218 [Paraburkholderia phymatum STM815] [TssK]
MMHDANMAVTDRIEWFEGMLLSPQHFQQMASRVDSLVAWQTLAAAPFSWGVRRLVLDHGLLPAGLLRVLELDAIMPDGTA
VTYSAEAQGAVRLELSLEPFAASLTSGPIDFYLVLPVAGTMRRSAQVKRFRSSAGMPVEDQVSDAPPADIPRLMPNLALA
AGELPSAMHVYLRLGSLYRDNEVVRLGEHLPPLLEVARDNPLWTSVASLLGQLRGKAAFVARQTANPSSKVDDRLTQLEL
KDRLRSLLSALPLAEAVLRTPHLHPLSLYLSLASLNGSLAMLKPGGLPPVPPDYDHADPLSVFTPLLRSLRESMSEVNEA
YREHKFEFRHGAFEIALHPDWIGERLVVGLRGQSDRDLLAWMDGAVIGSQSVYASLRSRRVLGAARRGIDYAEDLGLRSG
SGYLLFEVEAEHALVLASELLVIGNPNEGASAQRPQEMLLFVKG*

  Nucleotide sequence: 1335 bp    

>T6CP002746 NC_010625:527884-529218 [Paraburkholderia phymatum STM815] [TssK]
ATGATGCATGACGCCAACATGGCGGTGACCGACCGTATCGAATGGTTTGAAGGCATGCTGCTCTCGCCGCAGCATTTCCA
GCAAATGGCGTCGCGTGTCGATTCGCTCGTGGCCTGGCAGACGCTTGCCGCCGCGCCGTTCAGCTGGGGCGTCCGGCGTC
TGGTGTTGGACCACGGTCTGCTCCCCGCGGGTCTGCTGCGCGTGCTCGAACTCGACGCGATCATGCCCGACGGCACGGCT
GTGACGTATAGCGCCGAAGCGCAAGGCGCAGTGCGCCTTGAACTGTCGCTCGAACCCTTTGCGGCATCGCTCACGAGCGG
GCCGATCGATTTCTATCTTGTCCTGCCCGTCGCGGGCACGATGCGCCGCAGCGCCCAGGTCAAACGCTTCCGTTCGAGTG
CGGGTATGCCCGTCGAGGATCAGGTCTCTGACGCACCGCCCGCCGATATCCCTCGCCTCATGCCGAATCTCGCGCTTGCC
GCGGGCGAATTGCCGTCGGCCATGCATGTCTACCTGCGCCTCGGCTCGCTATACAGGGACAATGAGGTCGTACGGCTCGG
CGAGCATTTGCCACCGTTGCTGGAAGTGGCGCGCGACAATCCGCTGTGGACAAGCGTCGCGTCCCTGCTTGGGCAACTGC
GCGGCAAGGCGGCGTTCGTCGCGCGGCAGACTGCGAATCCTTCGTCGAAGGTCGACGATCGCCTGACGCAACTCGAACTC
AAGGACCGGCTGCGCAGCCTGCTGAGCGCGCTGCCGCTCGCAGAAGCCGTGCTGCGCACACCGCATCTTCATCCGCTGTC
GCTCTATCTGTCGCTGGCGTCGCTTAACGGATCGCTCGCGATGCTCAAGCCGGGTGGCCTGCCGCCCGTGCCGCCCGACT
ACGATCACGCGGACCCGCTTTCTGTGTTCACGCCGCTGTTGCGTTCGCTGCGCGAATCGATGTCGGAAGTCAACGAAGCG
TATCGCGAGCACAAGTTCGAATTCCGCCACGGGGCCTTCGAAATCGCGCTGCATCCGGACTGGATCGGCGAGCGTCTGGT
GGTCGGCCTGCGCGGTCAGTCGGATCGCGACCTGCTGGCGTGGATGGATGGCGCCGTCATCGGTTCTCAATCGGTCTATG
CGTCGTTGCGCAGCCGCCGCGTGCTGGGCGCCGCGCGCCGCGGGATCGACTATGCCGAGGATCTCGGCCTGCGTTCGGGC
TCGGGGTATCTGCTCTTCGAAGTCGAGGCCGAGCACGCGCTGGTGCTGGCGAGCGAACTGCTGGTGATCGGCAACCCCAA
CGAGGGCGCGAGCGCGCAGCGGCCACAGGAAATGCTGCTGTTCGTGAAGGGATGA