SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002744
Component protein type	TssH
T6SS ID (Type)	T6SS00274 (Type i4a)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (910 a.a.); Nucleotide sequence (2730 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS29915
Coordinate (Strand)	516865..519594 (+)
NCBI ID	WP_012405207.1
RefSeq	NC_010625
Uniprot ID	B2JVS0_PARP8
KEGG ID	bph:Bphy_5984
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AAA_2	PF07724.16	8.4e-38	637..801
AAA_lid_9	PF17871.3	8.3e-29	390..481
ClpB_D2-small	PF10431.11	2.1e-11	809..880
AAA	PF00004.31	9.2e-10	250..382

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-909	MDIDIRTLLSRLNPECKLAMEQAAQLCVRQTHYNVDVEHLLLTLLESDAPDLRAILAHFDIKPETLTAQLQKAVDLFKRGNGRTPALSPNFSPLFQEAWLLSSMLLGEQQVRSGTLILALLEVGSLRGMLLESAPALLKIPRASLREQIGMIVAGSAEDAGGAQARASAAPGAGQPGPASSAAAPQLDGGMPTMPSGATASQGRQTALDQYTVDLTGFAREGKIDPIRGRDAEIRQLIDVLLRRRQNNPILTGEAGVGKTAVVEGFAQRIVQGDVPPALANVSVRSLDLALLQAGAGVKGEFENRLKSVIAEVKASPAPVILFIDEAHQLIGAGGSEGQGDAANLLKPALARGELRTIAATTWAEYKKYVERDPALARRFQVVKVEEPSEAVAIEMLRGMVQKLEEHHGVEILDEAVRDAVKLSHRYISGRQLPDKAISVLDTACARVAIGQNGLPEDIEALGRSIETAENQLRIARHEAATGAERADVMAGLVKQLEDERAQHVRLTEKLTTEKRAVEEILAWRKKIRGYLTNQNDVLEAEDGESLTANLSRLEKGLEAVQNDEPMVPVCVDSETVAKVISGWTGIPVGRMLADELHTVLYLQDKLAERVVGQDEALDAIARRVRTFRADLDDPGKPVGVFLLVGPSGVGKTETAFALADMLYGGERNMITVNMSEFQEAHSVSGLKGAPPGYVGYGRGGVLTEAVRRRPYSVLLLDEMEKAHPDVLELFFQVFDKGVMEDGEGVPIDFKNTLILLTSNAAQDVITDACRGGRRPPPEELIEKLRPALLKQFSPAFLGRLVLVPYYHLGDAQINAIVNLKLERLAQRFSRNHHAPLTWDEGLATLIAQRCKEVDSGARNVDHILTQSVLPELARQVLERISISEPFGGVHLALSAAGDIAFRFLPKEA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9974	-	-

Protein sequence: 910 a.a.

						>T6CP002744 NC_010625:516865-519594 [Paraburkholderia phymatum STM815] [TssH]
MDIDIRTLLSRLNPECKLAMEQAAQLCVRQTHYNVDVEHLLLTLLESDAPDLRAILAHFDIKPETLTAQLQKAVDLFKRG
NGRTPALSPNFSPLFQEAWLLSSMLLGEQQVRSGTLILALLEVGSLRGMLLESAPALLKIPRASLREQIGMIVAGSAEDA
GGAQARASAAPGAGQPGPASSAAAPQLDGGMPTMPSGATASQGRQTALDQYTVDLTGFAREGKIDPIRGRDAEIRQLIDV
LLRRRQNNPILTGEAGVGKTAVVEGFAQRIVQGDVPPALANVSVRSLDLALLQAGAGVKGEFENRLKSVIAEVKASPAPV
ILFIDEAHQLIGAGGSEGQGDAANLLKPALARGELRTIAATTWAEYKKYVERDPALARRFQVVKVEEPSEAVAIEMLRGM
VQKLEEHHGVEILDEAVRDAVKLSHRYISGRQLPDKAISVLDTACARVAIGQNGLPEDIEALGRSIETAENQLRIARHEA
ATGAERADVMAGLVKQLEDERAQHVRLTEKLTTEKRAVEEILAWRKKIRGYLTNQNDVLEAEDGESLTANLSRLEKGLEA
VQNDEPMVPVCVDSETVAKVISGWTGIPVGRMLADELHTVLYLQDKLAERVVGQDEALDAIARRVRTFRADLDDPGKPVG
VFLLVGPSGVGKTETAFALADMLYGGERNMITVNMSEFQEAHSVSGLKGAPPGYVGYGRGGVLTEAVRRRPYSVLLLDEM
EKAHPDVLELFFQVFDKGVMEDGEGVPIDFKNTLILLTSNAAQDVITDACRGGRRPPPEELIEKLRPALLKQFSPAFLGR
LVLVPYYHLGDAQINAIVNLKLERLAQRFSRNHHAPLTWDEGLATLIAQRCKEVDSGARNVDHILTQSVLPELARQVLER
ISISEPFGGVHLALSAAGDIAFRFLPKEA*					

Nucleotide sequence: 2730 bp

						>T6CP002744 NC_010625:516865-519594 [Paraburkholderia phymatum STM815] [TssH]
ATGGACATCGATATCCGCACCCTGTTGAGCCGGCTCAATCCGGAATGCAAGCTTGCGATGGAGCAGGCGGCGCAACTGTG
TGTGCGTCAGACGCACTACAACGTCGACGTCGAGCATCTTCTGTTGACGCTGCTCGAATCCGATGCGCCTGACCTGCGCG
CCATTCTTGCGCACTTCGACATCAAACCCGAAACGCTCACCGCACAACTCCAGAAGGCCGTCGATCTTTTCAAGCGGGGC
AATGGCCGCACGCCCGCGCTGTCGCCGAATTTTTCTCCGCTCTTCCAGGAGGCGTGGCTGCTGAGTTCGATGCTCCTCGG
CGAGCAACAGGTCCGCTCGGGCACGCTCATCCTGGCGCTACTCGAAGTGGGCAGTCTGCGCGGCATGCTGCTTGAATCCG
CGCCCGCCTTGCTCAAGATTCCGCGCGCGAGCCTGCGCGAGCAGATCGGCATGATCGTCGCGGGCTCGGCGGAAGATGCG
GGCGGTGCGCAGGCGCGTGCGTCGGCCGCGCCGGGAGCGGGGCAACCGGGCCCCGCGAGCTCCGCTGCTGCACCGCAGTT
GGACGGCGGTATGCCGACCATGCCTTCTGGCGCTACCGCGAGCCAGGGTCGTCAAACCGCACTCGATCAATACACGGTCG
ATCTGACGGGGTTCGCCCGCGAGGGCAAAATTGACCCGATCCGTGGACGCGATGCGGAGATCCGGCAGCTGATCGATGTG
CTGCTGCGTCGCCGTCAGAACAACCCGATCCTGACGGGCGAGGCGGGAGTCGGGAAGACAGCCGTCGTCGAAGGTTTCGC
ACAGCGCATCGTTCAGGGCGACGTGCCGCCCGCGCTGGCCAACGTTTCCGTTCGTTCGCTCGATCTTGCGCTGTTGCAAG
CGGGAGCCGGGGTGAAGGGTGAGTTCGAGAACCGGCTCAAATCGGTGATCGCCGAGGTCAAAGCGTCGCCCGCGCCTGTG
ATTCTTTTCATTGACGAAGCGCACCAACTGATTGGCGCGGGCGGTAGCGAAGGCCAAGGCGACGCCGCGAACCTTCTCAA
GCCGGCATTGGCGCGTGGCGAACTGCGTACCATTGCCGCCACGACGTGGGCCGAATACAAGAAATACGTTGAGCGCGATC
CGGCGCTTGCGCGCCGCTTCCAGGTCGTCAAGGTCGAGGAGCCGAGTGAGGCTGTCGCGATCGAGATGCTGCGCGGCATG
GTTCAGAAACTGGAAGAACATCACGGCGTCGAGATTCTCGACGAAGCGGTGCGAGATGCCGTGAAGCTCTCGCACCGATA
CATCTCCGGGCGCCAGTTGCCGGACAAGGCGATCAGCGTGCTTGATACGGCCTGCGCGCGTGTCGCGATTGGTCAGAACG
GCTTGCCCGAGGACATCGAGGCACTGGGCCGTTCGATCGAGACGGCCGAAAACCAGCTTCGTATCGCGCGTCACGAAGCC
GCAACGGGCGCTGAGCGCGCCGACGTGATGGCTGGGCTGGTGAAGCAACTGGAAGACGAGCGCGCGCAGCACGTGCGTCT
CACCGAAAAGCTCACAACCGAAAAGCGGGCGGTGGAAGAGATCCTCGCATGGCGCAAGAAAATTCGAGGCTATCTGACGA
ATCAGAACGATGTGCTGGAAGCCGAAGATGGCGAGTCGCTGACGGCGAATCTCTCGCGACTGGAGAAAGGGCTCGAGGCC
GTGCAAAACGACGAGCCGATGGTCCCCGTGTGTGTCGATTCGGAAACGGTCGCGAAGGTGATATCCGGCTGGACGGGCAT
TCCTGTTGGCCGGATGCTCGCAGATGAGCTCCACACCGTGCTGTATCTGCAGGACAAGCTTGCGGAACGCGTCGTCGGCC
AGGACGAGGCGCTTGACGCCATTGCGCGCCGGGTGCGCACCTTCCGTGCGGATCTCGACGATCCAGGCAAGCCCGTTGGC
GTCTTCCTGCTCGTCGGACCCAGCGGCGTCGGCAAGACGGAAACGGCGTTTGCGCTTGCCGACATGCTGTACGGCGGCGA
ACGCAACATGATCACCGTCAATATGTCCGAGTTCCAGGAGGCGCATAGCGTTTCGGGACTCAAGGGCGCGCCGCCGGGCT
ATGTCGGATACGGCCGCGGCGGCGTGCTGACCGAGGCGGTGCGACGCCGCCCCTACAGCGTGCTGCTGCTCGACGAAATG
GAGAAGGCGCATCCCGACGTGCTCGAGCTATTCTTCCAGGTGTTCGACAAGGGCGTGATGGAGGACGGCGAGGGCGTTCC
CATCGACTTCAAGAACACGCTGATCCTGCTTACGTCGAATGCCGCGCAGGATGTGATCACCGACGCATGCCGGGGCGGGC
GCCGCCCGCCGCCCGAAGAGCTGATAGAGAAACTGCGCCCCGCGCTCCTCAAACAGTTCAGCCCCGCATTCCTCGGCCGG
CTCGTACTCGTGCCGTACTACCACCTCGGCGACGCGCAGATCAACGCAATCGTCAACCTCAAGCTCGAACGGCTCGCGCA
GCGATTTTCGCGTAACCATCACGCGCCCCTCACGTGGGACGAAGGTCTCGCCACGCTGATCGCGCAACGCTGCAAGGAAG
TGGACAGCGGCGCGCGCAACGTCGACCACATTCTTACGCAGTCGGTGCTTCCGGAACTGGCGCGCCAGGTGCTCGAGAGA
ATCTCGATATCGGAGCCGTTCGGCGGCGTGCATCTTGCGCTGAGCGCAGCGGGCGACATCGCGTTCCGCTTCCTGCCCAA
GGAGGCGTAA					