SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002747
Component protein type	TssL
T6SS ID (Type)	T6SS00274 (Type i4a)
Strain	Paraburkholderia phymatum STM815
Replicon	plasmid pBPHY01
Sequence	Protein sequence (251 a.a.); Nucleotide sequence (753 bp)
Reference	[1] de Campos SB, Lardi M, Gandolfi A, et al. Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition. Front Microbiol. 2017 Dec 12;8:2473. doi: 10.3389/fmicb.2017.02473. eCollection 2017. PMID: 29312183 [2] Hug S, Liu Y, Heiniger B, et al. Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction. Front Plant Sci. 2021 Jul 28;12:699590. doi: 10.3389/fpls.2021.699590. eCollection 2021. PMID: 34394152

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPHY_RS29970
Coordinate (Strand)	529280..530032 (+)
NCBI ID	WP_012405218.1
RefSeq	NC_010625
Uniprot ID	B2JVT1_PARP8
KEGG ID	bph:Bphy_5995
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	2.1e-35	21..223

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-209	MARNRPEPEEDDLVTEQFRTFLDELVRAQTRFSGLPDADPDLAAQSMSRHLLDLLEIQTLQSRRDSTRFEMETIADARYLKAVLADEILLNSPWAGREHWTGYLLESTLFRTNVAGDQIFRRIEQLLSDREPSYRNIARLYLFALAMGFQGRFRDTDATARLRSYREELYEFVYQRRPDLGGRERVLSEPAYGNTLSHVAPRKLPTVSR
Transmembrane helix	210-232	WAVILMLAAVSLLAISEMLWLWQ
Outside	233-250	SWPVRDALHADPVTGRSS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9947	-	-

Protein sequence: 251 a.a.

						>T6CP002747 NC_010625:529280-530032 [Paraburkholderia phymatum STM815] [TssL]
MARNRPEPEEDDLVTEQFRTFLDELVRAQTRFSGLPDADPDLAAQSMSRHLLDLLEIQTLQSRRDSTRFEMETIADARYL
KAVLADEILLNSPWAGREHWTGYLLESTLFRTNVAGDQIFRRIEQLLSDREPSYRNIARLYLFALAMGFQGRFRDTDATA
RLRSYREELYEFVYQRRPDLGGRERVLSEPAYGNTLSHVAPRKLPTVSRWAVILMLAAVSLLAISEMLWLWQSWPVRDAL
HADPVTGRSS*					

Nucleotide sequence: 753 bp

						>T6CP002747 NC_010625:529280-530032 [Paraburkholderia phymatum STM815] [TssL]
ATGGCACGCAACCGACCCGAACCCGAAGAAGACGATCTTGTGACCGAGCAGTTCCGTACGTTTCTCGACGAGCTCGTCAG
GGCGCAGACGCGATTTTCCGGGTTGCCCGATGCAGACCCGGACCTCGCCGCGCAGAGCATGAGTCGCCACCTGCTTGATC
TGCTCGAAATCCAGACGCTGCAATCGCGCCGTGACAGCACGCGCTTCGAAATGGAGACGATTGCCGACGCCCGCTATCTG
AAAGCCGTGCTCGCAGACGAAATCCTGCTCAATTCGCCGTGGGCGGGACGCGAGCACTGGACGGGCTATCTGCTCGAATC
GACGCTGTTTCGCACCAACGTCGCCGGAGATCAGATCTTTCGCCGTATCGAGCAATTGCTGTCGGACCGCGAACCTTCAT
ATCGCAATATCGCGCGGCTCTACCTGTTCGCGCTGGCGATGGGCTTCCAGGGCCGCTTTCGCGACACCGATGCCACGGCG
CGTCTGCGCAGCTATCGAGAGGAACTCTACGAGTTCGTCTATCAGCGCCGCCCCGATCTTGGGGGGCGCGAGCGCGTGCT
GTCCGAGCCCGCCTATGGCAACACGCTGTCGCACGTTGCTCCGCGCAAGCTGCCGACAGTCAGCCGCTGGGCCGTGATTC
TGATGCTCGCCGCAGTATCACTGCTGGCGATTTCCGAAATGCTGTGGCTGTGGCAATCGTGGCCCGTACGCGACGCGCTG
CATGCCGACCCGGTGACGGGGAGAAGTTCATGA					