Detailed information of component protein


Summary

Component ID T6CP002749
Component protein type TssA
T6SS ID (Type) T6SS00274 experimental (Type i4a)
Strain Paraburkholderia phymatum STM815
Replicon plasmid pBPHY01
Sequence Protein sequence (383 a.a.); Nucleotide sequence (1149 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPHY_RS29980
Coordinate (Strand) 534296..535444 (+)
NCBI ID WP_012405220.1
RefSeq NC_010625
Uniprot ID B2JVT3_PARP8
KEGG ID bph:Bphy_5997
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 3.6e-34 21..144
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-382 MKQSYPMDIAQGLDIDLEALLAPVPMGEGAGVSLRYEPLYQHIRDARTHDDSSVPMGEWERPLIKADWKRVAASCTEALSTRSKDFQLAAWLCEAWTHMHRIEGFIAGTRLLTMLAESYWEHAWPALEEGDTDARVAPFVWLNDTIALVLTLHVPLLVIEGREPANVNLDEWQRVLVEGGDDDAADLTRDLLDKHVTQGGNLASLAALHQQLEVAQQMWLSFGRLLDVLLQGDSPHLGRVSDVLMRLSRAVTSLLGNHALAAATGQVPHDDPAAGLMAVGGAPSVNENYALLETQMSDALREPPVVGTSIRALPARVESRAQAYQLIEVIAQYLAEQEPHSPTPYLLRRAASWGQMPLPELMREVLRTEGDMSRYLAMLEIK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9908 - -


  Protein sequence: 383 a.a.    

>T6CP002749 NC_010625:534296-535444 [Paraburkholderia phymatum STM815] [TssA]
MKQSYPMDIAQGLDIDLEALLAPVPMGEGAGVSLRYEPLYQHIRDARTHDDSSVPMGEWERPLIKADWKRVAASCTEALS
TRSKDFQLAAWLCEAWTHMHRIEGFIAGTRLLTMLAESYWEHAWPALEEGDTDARVAPFVWLNDTIALVLTLHVPLLVIE
GREPANVNLDEWQRVLVEGGDDDAADLTRDLLDKHVTQGGNLASLAALHQQLEVAQQMWLSFGRLLDVLLQGDSPHLGRV
SDVLMRLSRAVTSLLGNHALAAATGQVPHDDPAAGLMAVGGAPSVNENYALLETQMSDALREPPVVGTSIRALPARVESR
AQAYQLIEVIAQYLAEQEPHSPTPYLLRRAASWGQMPLPELMREVLRTEGDMSRYLAMLEIK*

  Nucleotide sequence: 1149 bp    

>T6CP002749 NC_010625:534296-535444 [Paraburkholderia phymatum STM815] [TssA]
ATGAAACAGTCGTATCCGATGGACATCGCACAAGGCCTCGACATCGACCTGGAGGCGCTGCTTGCGCCCGTGCCAATGGG
CGAGGGCGCCGGCGTCTCGCTACGCTACGAGCCGCTGTACCAGCACATCCGCGACGCTCGCACGCATGACGACTCGAGCG
TCCCGATGGGCGAATGGGAACGTCCGTTGATCAAGGCGGACTGGAAGCGCGTGGCAGCCTCATGCACGGAGGCGCTCAGC
ACGCGCAGCAAGGATTTCCAGCTTGCCGCATGGTTGTGCGAAGCATGGACGCATATGCACCGCATCGAAGGTTTTATCGC
GGGGACGAGGCTGCTGACGATGCTCGCGGAAAGCTATTGGGAACACGCCTGGCCGGCGCTGGAAGAAGGCGACACGGATG
CGCGTGTCGCGCCGTTCGTATGGCTGAACGACACGATCGCCCTTGTGCTGACGTTGCACGTGCCGCTCCTCGTGATCGAA
GGGCGCGAGCCGGCAAACGTGAATCTGGACGAGTGGCAGCGCGTCCTCGTGGAAGGAGGCGATGACGATGCGGCTGACCT
CACGCGAGACCTGCTCGACAAACACGTCACGCAAGGAGGCAACCTGGCTAGCCTGGCGGCGCTGCATCAGCAGCTCGAGG
TCGCCCAGCAGATGTGGCTGTCGTTCGGCCGCCTGCTCGACGTTCTGCTGCAGGGCGATTCGCCTCATCTGGGCAGGGTG
AGCGATGTGCTGATGCGCCTCTCGCGGGCGGTCACAAGCCTGCTCGGCAATCACGCATTGGCCGCTGCCACGGGTCAAGT
TCCACATGACGATCCTGCGGCGGGCTTGATGGCCGTGGGGGGTGCACCGTCGGTCAACGAAAATTACGCATTGCTGGAGA
CGCAAATGAGTGACGCATTGCGAGAGCCGCCTGTCGTGGGCACGTCAATCAGAGCTCTTCCCGCCCGAGTGGAGAGCAGG
GCACAAGCTTATCAACTGATCGAAGTCATCGCGCAGTACCTGGCCGAACAAGAACCGCATAGTCCGACGCCGTATCTGCT
ACGTCGTGCCGCTTCGTGGGGGCAAATGCCGCTGCCCGAGCTGATGCGGGAGGTGTTGCGCACGGAAGGCGACATGTCGC
GCTATCTCGCGATGCTCGAGATCAAGTAA