Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   ABFU40_RS05410 Genome accession   NZ_CP155932
Coordinates   1285648..1286088 (+) Length   146 a.a.
NCBI ID   WP_011038214.1    Uniprot ID   Q8P671
Organism   Xanthomonas campestris pv. campestris strain CFBP 1712     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1280648..1291088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU40_RS05380 (ABFU40_05380) - 1280772..1281263 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  ABFU40_RS05385 (ABFU40_05385) - 1281260..1281550 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  ABFU40_RS05390 (ABFU40_05390) - 1281625..1282026 (-) 402 WP_019237348.1 SymE family type I addiction module toxin -
  ABFU40_RS05395 (ABFU40_05395) coaE 1282558..1283181 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  ABFU40_RS05400 (ABFU40_05400) - 1283195..1284058 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU40_RS05405 (ABFU40_05405) pilC 1284065..1285321 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  ABFU40_RS05410 (ABFU40_05410) pilE 1285648..1286088 (+) 441 WP_011038214.1 pilin Machinery gene
  ABFU40_RS05415 (ABFU40_05415) - 1286201..1286602 (+) 402 WP_194734302.1 pilin -
  ABFU40_RS05420 (ABFU40_05420) pilB 1286667..1288400 (+) 1734 WP_011038212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU40_RS05430 (ABFU40_05430) pilR 1288819..1290213 (-) 1395 WP_221261830.1 sigma-54 dependent transcriptional regulator Regulator

Sequence


Protein


Download         Length: 146 a.a.        Molecular weight: 14797.93 Da        Isoelectric Point: 9.0090

>NTDB_id=999841 ABFU40_RS05410 WP_011038214.1 1285648..1286088(+) (pilE) [Xanthomonas campestris pv. campestris strain CFBP 1712]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYLARSQVSEGLSLASGAKTAVAETYANTGAFPATNAAAGLEAAANIKG
KYVTSVTVGAGGIITAAFNTANAKLSGKNLVLTPTDNNGSISWGCTNGTTIDQKYLPTSCRTAAAP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=999841 ABFU40_RS05410 WP_011038214.1 1285648..1286088(+) (pilE) [Xanthomonas campestris pv. campestris strain CFBP 1712]
ATGAAAAAGCAAAATGGTTTTACACTGATCGAACTCATGATCGTCGTTGCGATCATCGCTATTCTGGCCGCTATCGCTTT
GCCGGCGTACCAGGATTACCTCGCTCGTTCGCAGGTTTCGGAAGGCTTGTCTTTGGCGTCGGGTGCAAAGACAGCTGTCG
CTGAAACTTATGCTAACACCGGTGCCTTCCCGGCGACCAATGCCGCCGCTGGCCTTGAGGCTGCTGCGAACATCAAGGGT
AAGTACGTTACGTCTGTGACGGTGGGGGCGGGTGGGATCATCACCGCGGCATTCAATACTGCTAATGCAAAGTTGAGTGG
CAAGAATCTGGTCCTGACCCCGACTGACAATAACGGTTCGATCAGCTGGGGGTGCACCAACGGCACCACGATTGATCAGA
AGTATCTGCCGACCTCTTGCCGCACTGCGGCGGCTCCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB Q8P671

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

46.875

100

0.514

  pilE Neisseria gonorrhoeae strain FA1090

46.541

100

0.507

  pilA2 Legionella pneumophila str. Paris

52.857

95.89

0.507

  pilA2 Legionella pneumophila strain ERS1305867

52.143

95.89

0.5

  pilA/pilA1 Eikenella corrodens VA1

46.452

100

0.493

  comP Acinetobacter baylyi ADP1

48

100

0.493

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.473

  pilA Ralstonia pseudosolanacearum GMI1000

40.244

100

0.452

  pilA Acinetobacter baumannii strain A118

41.333

100

0.425

  pilA Pseudomonas aeruginosa PAK

40.789

100

0.425

  pilA Vibrio campbellii strain DS40M4

40.268

100

0.411

  pilA Vibrio cholerae C6706

38.411

100

0.397

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

100

0.397

  pilA Vibrio cholerae strain A1552

38.411

100

0.397

  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.288

95.205

0.384

  pilA Vibrio parahaemolyticus RIMD 2210633

42.4

85.616

0.363


Multiple sequence alignment