Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFU19_RS14725 Genome accession   NZ_CP155928
Coordinates   3390189..3391226 (-) Length   345 a.a.
NCBI ID   WP_042823132.1    Uniprot ID   A0A098Q1D4
Organism   Xanthomonas campestris strain CFBP 13427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3385189..3396226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU19_RS14705 (ABFU19_14680) - 3385582..3386844 (+) 1263 WP_119960392.1 hypothetical protein -
  ABFU19_RS14710 (ABFU19_14685) - 3387069..3387968 (+) 900 WP_119959319.1 DUF72 domain-containing protein -
  ABFU19_RS14715 (ABFU19_14690) - 3387982..3388656 (-) 675 WP_183111560.1 YitT family protein -
  ABFU19_RS14720 (ABFU19_14695) pilU 3388936..3390066 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU19_RS14725 (ABFU19_14700) pilT 3390189..3391226 (-) 1038 WP_042823132.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU19_RS14730 (ABFU19_14705) - 3391513..3392196 (+) 684 WP_119959318.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU19_RS14735 (ABFU19_14710) proC 3392237..3393097 (+) 861 WP_183111038.1 pyrroline-5-carboxylate reductase -
  ABFU19_RS14740 (ABFU19_14715) - 3393638..3394060 (+) 423 WP_040940938.1 HU family DNA-binding protein -
  ABFU19_RS14745 (ABFU19_14720) - 3394204..3394794 (+) 591 WP_119959316.1 M23 family metallopeptidase -
  ABFU19_RS14750 (ABFU19_14725) - 3394887..3395195 (+) 309 WP_014508439.1 DUF3861 domain-containing protein -
  ABFU19_RS14755 (ABFU19_14730) - 3395249..3395788 (+) 540 WP_119959315.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38629.27 Da        Isoelectric Point: 6.6650

>NTDB_id=999775 ABFU19_RS14725 WP_042823132.1 3390189..3391226(-) (pilT) [Xanthomonas campestris strain CFBP 13427]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=999775 ABFU19_RS14725 WP_042823132.1 3390189..3391226(-) (pilT) [Xanthomonas campestris strain CFBP 13427]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGTTGATCGACCAACCGCAGGGTCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCTGCGGAAACCGGCCATCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGATTATGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Q1D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397

Multiple sequence alignment