Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU19_RS14720 Genome accession   NZ_CP155928
Coordinates   3388936..3390066 (-) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas campestris strain CFBP 13427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3383936..3395066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU19_RS14700 (ABFU19_14675) - 3385046..3385585 (+) 540 WP_119959320.1 DNA-3-methyladenine glycosylase I -
  ABFU19_RS14705 (ABFU19_14680) - 3385582..3386844 (+) 1263 WP_119960392.1 hypothetical protein -
  ABFU19_RS14710 (ABFU19_14685) - 3387069..3387968 (+) 900 WP_119959319.1 DUF72 domain-containing protein -
  ABFU19_RS14715 (ABFU19_14690) - 3387982..3388656 (-) 675 WP_183111560.1 YitT family protein -
  ABFU19_RS14720 (ABFU19_14695) pilU 3388936..3390066 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU19_RS14725 (ABFU19_14700) pilT 3390189..3391226 (-) 1038 WP_042823132.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU19_RS14730 (ABFU19_14705) - 3391513..3392196 (+) 684 WP_119959318.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU19_RS14735 (ABFU19_14710) proC 3392237..3393097 (+) 861 WP_183111038.1 pyrroline-5-carboxylate reductase -
  ABFU19_RS14740 (ABFU19_14715) - 3393638..3394060 (+) 423 WP_040940938.1 HU family DNA-binding protein -
  ABFU19_RS14745 (ABFU19_14720) - 3394204..3394794 (+) 591 WP_119959316.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=999774 ABFU19_RS14720 WP_005990431.1 3388936..3390066(-) (pilU) [Xanthomonas campestris strain CFBP 13427]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=999774 ABFU19_RS14720 WP_005990431.1 3388936..3390066(-) (pilU) [Xanthomonas campestris strain CFBP 13427]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATTTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGTTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGCTGCGGCGGATCGAGACGCGCATTCC
GACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACT
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAATGCGTTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGTACGCTGCACGCCAACAACGCCAACCAAGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAACCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGTTATGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCTAAGACGCTGTCGCAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment