Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAHK14_RS02940 Genome accession   NZ_CP155576
Coordinates   548402..549529 (+) Length   375 a.a.
NCBI ID   WP_194092644.1    Uniprot ID   -
Organism   Moraxella sp. K1664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543402..554529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHK14_RS02910 (AAHK14_02910) - 543646..544293 (-) 648 WP_062499971.1 UPF0149 family protein -
  AAHK14_RS02915 (AAHK14_02915) - 544452..544817 (+) 366 WP_065256233.1 hypothetical protein -
  AAHK14_RS02920 (AAHK14_02920) - 544838..545500 (+) 663 WP_227712976.1 cell division protein ZapA -
  AAHK14_RS02925 (AAHK14_02925) - 545608..546483 (+) 876 WP_065256234.1 PoNe immunity protein domain-containing protein -
  AAHK14_RS02930 (AAHK14_02930) - 546474..547178 (-) 705 WP_065256235.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAHK14_RS02935 (AAHK14_02935) pilT 547346..548392 (+) 1047 WP_065256236.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHK14_RS02940 (AAHK14_02940) pilU 548402..549529 (+) 1128 WP_194092644.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAHK14_RS02945 (AAHK14_02945) fur 549588..550034 (-) 447 WP_062499981.1 ferric iron uptake transcriptional regulator -
  AAHK14_RS02950 (AAHK14_02950) - 550228..551271 (-) 1044 WP_172823646.1 cytochrome c peroxidase -
  AAHK14_RS02955 (AAHK14_02955) - 551404..552159 (-) 756 WP_083108295.1 alpha/beta hydrolase-fold protein -
  AAHK14_RS02960 (AAHK14_02960) - 552156..552296 (-) 141 WP_156065143.1 hypothetical protein -
  AAHK14_RS02965 (AAHK14_02965) - 552532..553419 (-) 888 WP_065256241.1 class II glutamine amidotransferase -
  AAHK14_RS02970 (AAHK14_02970) - 553538..554308 (-) 771 WP_065256242.1 DUF3800 domain-containing protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41885.31 Da        Isoelectric Point: 6.5796

>NTDB_id=997912 AAHK14_RS02940 WP_194092644.1 548402..549529(+) (pilU) [Moraxella sp. K1664]
MDFDKLLQVMIAKNGSDLFITEGVPPSLKVNGTILPMTKTPLTAEQTMALVTSIMTEAQKKEFHETNECQFAISDKAETA
RFRVSAMIQRNKAAMVLRKIETQIPTTEDLKLPPILKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNENSRGHIITIE
DPIEYVHQHKGCIVTQREVGIDTLSFEEGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLRAIIAQQLIPTPDGKGRRAAIEILLGTQLIADYIRKGEIHEIKPVMKRSRDIGMQTFDQ
ALFDLYESGQITYQDALKHADSPNDLRLQIKLESKNAAAVEEESAKLSIDMGDYP

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=997912 AAHK14_RS02940 WP_194092644.1 548402..549529(+) (pilU) [Moraxella sp. K1664]
ATGGATTTTGATAAACTACTACAAGTGATGATTGCCAAAAACGGCTCTGACCTTTTTATCACCGAAGGCGTTCCCCCGTC
ACTCAAAGTCAATGGTACCATTTTGCCCATGACCAAAACGCCACTGACTGCTGAGCAAACCATGGCACTGGTGACCAGTA
TCATGACCGAAGCTCAAAAAAAAGAGTTCCATGAGACCAACGAGTGCCAATTTGCCATCTCTGATAAAGCCGAAACCGCT
CGTTTTCGTGTGTCAGCGATGATTCAACGTAACAAGGCGGCGATGGTGTTGCGTAAGATTGAGACGCAAATTCCCACGAC
CGAAGATCTAAAACTACCGCCGATTCTAAAAGAGCTTGCCATGAAAAAGCGTGGCATTATCCTACTTGTCGGTGCGACAG
GTACAGGTAAATCCACCACGCTGGCTGCCATGATTGGACATCGTAACGAGAACTCTCGTGGTCACATCATCACCATCGAA
GACCCGATTGAATACGTCCATCAGCACAAAGGCTGTATCGTCACTCAGCGTGAAGTGGGTATTGACACACTCTCGTTTGA
AGAAGGGCTAAAAAACACCCTACGTCAAGCCCCTGATGTTATCCTTATCGGCGAGATTCGTAACCGTGAAGTCATGAGCT
ATGCCATCCAGTACGCTGAGACGGGTCACTTGGTATTTGCCACACTCCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATCATTCACTTTTTTGAAGCGGACAGACACGGACAGTTATTCATGGACTTGTCACTCAACTTGCGTGCCATCATCGCCCA
ACAGCTTATCCCAACCCCTGATGGCAAAGGTCGTCGTGCCGCCATCGAGATTCTACTAGGCACACAGCTCATCGCTGACT
ACATCCGTAAGGGCGAGATTCATGAGATTAAGCCTGTGATGAAACGCTCTCGTGACATCGGCATGCAGACCTTTGACCAA
GCGTTGTTTGACCTGTATGAATCAGGGCAAATTACCTATCAAGACGCACTCAAACACGCTGACAGCCCGAACGACCTACG
CTTACAAATCAAACTGGAAAGCAAAAATGCTGCCGCGGTCGAAGAAGAGTCTGCCAAACTCTCTATTGATATGGGCGACT
ACCCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.38

99.733

0.672
  pilU Acinetobacter baylyi ADP1

68.182

93.867

0.64
  pilU Vibrio cholerae strain A1552

54.416

93.6

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.816

92.8

0.397
  pilT Legionella pneumophila strain Lp02

43.452

89.6

0.389
  pilT Legionella pneumophila strain ERS1305867

43.452

89.6

0.389
  pilT Neisseria meningitidis 8013

40.346

92.533

0.373
  pilT Acinetobacter baumannii D1279779

40.816

91.467

0.373
  pilT Acinetobacter nosocomialis M2

40.816

91.467

0.373
  pilT Acinetobacter baumannii strain A118

40.816

91.467

0.373
  pilT Pseudomonas aeruginosa PAK

40.173

92.267

0.371
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.267

0.365
  pilT Neisseria gonorrhoeae MS11

39.595

92.267

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.597

89.333

0.363
  pilT Vibrio cholerae strain A1552

40.597

89.333

0.363
  pilT Acinetobacter baylyi ADP1

39.359

91.467

0.36

Multiple sequence alignment