Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAHK14_RS02935 Genome accession   NZ_CP155576
Coordinates   547346..548392 (+) Length   348 a.a.
NCBI ID   WP_065256236.1    Uniprot ID   A0A1B8PZ08
Organism   Moraxella sp. K1664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542346..553392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHK14_RS02910 (AAHK14_02910) - 543646..544293 (-) 648 WP_062499971.1 UPF0149 family protein -
  AAHK14_RS02915 (AAHK14_02915) - 544452..544817 (+) 366 WP_065256233.1 hypothetical protein -
  AAHK14_RS02920 (AAHK14_02920) - 544838..545500 (+) 663 WP_227712976.1 cell division protein ZapA -
  AAHK14_RS02925 (AAHK14_02925) - 545608..546483 (+) 876 WP_065256234.1 PoNe immunity protein domain-containing protein -
  AAHK14_RS02930 (AAHK14_02930) - 546474..547178 (-) 705 WP_065256235.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAHK14_RS02935 (AAHK14_02935) pilT 547346..548392 (+) 1047 WP_065256236.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHK14_RS02940 (AAHK14_02940) pilU 548402..549529 (+) 1128 WP_194092644.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAHK14_RS02945 (AAHK14_02945) fur 549588..550034 (-) 447 WP_062499981.1 ferric iron uptake transcriptional regulator -
  AAHK14_RS02950 (AAHK14_02950) - 550228..551271 (-) 1044 WP_172823646.1 cytochrome c peroxidase -
  AAHK14_RS02955 (AAHK14_02955) - 551404..552159 (-) 756 WP_083108295.1 alpha/beta hydrolase-fold protein -
  AAHK14_RS02960 (AAHK14_02960) - 552156..552296 (-) 141 WP_156065143.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38441.32 Da        Isoelectric Point: 6.9913

>NTDB_id=997911 AAHK14_RS02935 WP_065256236.1 547346..548392(+) (pilT) [Moraxella sp. K1664]
MSAPSIEDLLRFVVKNKASDLHLSAGLPPMIRVDGEVVRINTPEMDHSTVHKLIYDIMNDKQRADYEEFLETDFSFEIPG
LARFRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGPVFKKVSDFKRGVVLVTGPTGSGKSTTLAAMINYINESRKEHILT
IEDPIEFVHESKKSLVNQREVHRDTLGFDAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTNSAAKTI
DRVIDVFPAAEKDMIRAMLSESLQAVISQALLKKVGGGRVAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGAGEGMITLD
QTLKNLVASRTITKETARVYAKQPEAFL

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=997911 AAHK14_RS02935 WP_065256236.1 547346..548392(+) (pilT) [Moraxella sp. K1664]
ATGTCAGCTCCTAGTATTGAAGACCTACTGCGTTTTGTGGTAAAAAATAAAGCGTCCGACTTGCACTTATCGGCAGGACT
ACCACCCATGATTCGTGTGGATGGTGAAGTGGTGCGTATCAACACCCCCGAGATGGATCACTCTACCGTGCATAAGCTCA
TTTATGACATCATGAATGACAAACAGCGTGCCGACTACGAAGAGTTTTTAGAAACGGACTTCTCGTTTGAAATCCCCGGG
CTTGCTCGTTTTCGTGTGAACGCCTTTAACCAAAACCGTGGTGCTGGTGCGGTATTTCGTACCATTCCATCCAAGGTTCT
GACCATGGAAGATTTGGGCATGGGTCCTGTCTTTAAAAAGGTCTCTGACTTTAAGCGTGGCGTGGTACTGGTCACAGGCC
CGACAGGTTCGGGCAAATCAACCACACTGGCGGCGATGATTAACTACATCAACGAAAGCCGTAAAGAGCATATCCTAACC
ATCGAAGACCCCATCGAGTTTGTCCACGAATCCAAAAAATCGCTCGTGAACCAACGTGAAGTACACCGTGATACCCTAGG
CTTTGATGCTGCTTTGCGTTCGGCACTGCGTGAAGACCCCGACATCATTCTGGTCGGTGAGATGCGTGACTTGGAGACCA
TCCGCCTTGCCTTGACTGCTGCCGAGACAGGACACTTGGTGTTTGGGACACTCCACACCAACTCTGCTGCCAAGACCATT
GACCGTGTGATTGACGTATTCCCTGCCGCCGAAAAAGACATGATTCGTGCGATGTTATCCGAGTCCTTGCAAGCAGTTAT
CTCGCAAGCCCTACTCAAAAAAGTCGGGGGCGGTCGTGTGGCGGCTCATGAGATTATGCTTGGCACACCTGCCATTCGTA
ACCTTATCCGTGAAAACAAAATCGCCCAAATGTACTCTGCCATCCAAACAGGGGCAGGCGAAGGCATGATTACCCTTGAC
CAGACGTTAAAGAACTTGGTCGCGTCTCGCACCATCACCAAAGAGACGGCACGTGTCTATGCCAAACAGCCAGAAGCATT
CTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B8PZ08

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

81.633

98.563

0.805
  pilT Acinetobacter baumannii strain A118

81.633

98.563

0.805
  pilT Acinetobacter nosocomialis M2

81.341

98.563

0.802
  pilT Pseudomonas stutzeri DSM 10701

80.409

98.276

0.79
  pilT Pseudomonas aeruginosa PAK

80.409

98.276

0.79
  pilT Acinetobacter baylyi ADP1

79.532

98.276

0.782
  pilT Legionella pneumophila strain Lp02

73.684

98.276

0.724
  pilT Legionella pneumophila strain ERS1305867

73.684

98.276

0.724
  pilT Neisseria meningitidis 8013

66.181

98.563

0.652
  pilT Neisseria gonorrhoeae MS11

65.889

98.563

0.649
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.659

92.816

0.647
  pilT Vibrio cholerae strain A1552

69.659

92.816

0.647
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.873

99.713

0.517
  pilU Pseudomonas stutzeri DSM 10701

42.522

97.989

0.417
  pilU Acinetobacter baylyi ADP1

40.708

97.414

0.397
  pilU Vibrio cholerae strain A1552

41.411

93.678

0.388

Multiple sequence alignment