Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABER85_RS16025 Genome accession   NZ_CP155547
Coordinates   3326695..3327732 (-) Length   345 a.a.
NCBI ID   WP_346534912.1    Uniprot ID   -
Organism   Acinetobacter sp. 8_A20231192     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3321695..3332732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABER85_RS15995 (ABER85_15995) - 3322479..3322934 (+) 456 WP_000782976.1 bacteriohemerythrin -
  ABER85_RS16000 (ABER85_16000) - 3323087..3324154 (+) 1068 WP_004748097.1 hypothetical protein -
  ABER85_RS16005 (ABER85_16005) - 3324154..3324477 (+) 324 WP_000442590.1 RnfH family protein -
  ABER85_RS16010 (ABER85_16010) bamE 3324532..3324930 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  ABER85_RS16015 (ABER85_16015) fur 3325042..3325479 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  ABER85_RS16020 (ABER85_16020) pilU 3325549..3326667 (-) 1119 WP_000347034.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABER85_RS16025 (ABER85_16025) pilT 3326695..3327732 (-) 1038 WP_346534912.1 type IV pilus twitching motility protein PilT Machinery gene
  ABER85_RS16030 (ABER85_16030) - 3327859..3328551 (+) 693 WP_001108509.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABER85_RS16035 (ABER85_16035) - 3328753..3332349 (-) 3597 WP_104159727.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38412.05 Da        Isoelectric Point: 6.8888

>NTDB_id=997678 ABER85_RS16025 WP_346534912.1 3326695..3327732(-) (pilT) [Acinetobacter sp. 8_A20231192]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVREMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=997678 ABER85_RS16025 WP_346534912.1 3326695..3327732(-) (pilT) [Acinetobacter sp. 8_A20231192]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTCTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAC
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCAGATATTATTTTAGTCCGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCCCATGAAATTATGATTGGTATTCCGGCTATTCGTAATTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTAGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGTCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

99.71

100

0.997
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.721

99.71

0.835
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.101

0.391
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment