Detailed information NTG0997677

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABER85_RS16020
• Genome accession: NZ_CP155547
• Coordinates: 3325549..3326667 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter sp. 8_A20231192
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3320549..3331667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABER85_RS15990 (ABER85_15990)	-	3321378..3322226 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
ABER85_RS15995 (ABER85_15995)	-	3322479..3322934 (+)	456	WP_000782976.1	bacteriohemerythrin	-
ABER85_RS16000 (ABER85_16000)	-	3323087..3324154 (+)	1068	WP_004748097.1	hypothetical protein	-
ABER85_RS16005 (ABER85_16005)	-	3324154..3324477 (+)	324	WP_000442590.1	RnfH family protein	-
ABER85_RS16010 (ABER85_16010)	bamE	3324532..3324930 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
ABER85_RS16015 (ABER85_16015)	fur	3325042..3325479 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
ABER85_RS16020 (ABER85_16020)	pilU	3325549..3326667 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABER85_RS16025 (ABER85_16025)	pilT	3326695..3327732 (-)	1038	WP_346534912.1	type IV pilus twitching motility protein PilT	Machinery gene
ABER85_RS16030 (ABER85_16030)	-	3327859..3328551 (+)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=997677 ABER85_RS16020 WP_000347034.1 3325549..3326667(-) (pilU) [Acinetobacter sp. 8_A20231192]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=997677 ABER85_RS16020 WP_000347034.1 3325549..3326667(-) (pilU) [Acinetobacter sp. 8_A20231192]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39