Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMN23_RS10400 Genome accession   NZ_AP027151
Coordinates   2243623..2244717 (+) Length   364 a.a.
NCBI ID   WP_281999247.1    Uniprot ID   -
Organism   Geotalea uraniireducens strain NIT-SL11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2238623..2249717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMN23_RS10385 (GURASL_20510) - 2239104..2240978 (+) 1875 WP_281999245.1 DUF2723 domain-containing protein -
  QMN23_RS10390 (GURASL_20520) - 2240978..2241841 (+) 864 WP_282003854.1 shikimate dehydrogenase -
  QMN23_RS10395 (GURASL_20530) pilB 2241902..2243608 (+) 1707 WP_281999246.1 type IV-A pilus assembly ATPase PilB -
  QMN23_RS10400 (GURASL_20540) pilT 2243623..2244717 (+) 1095 WP_281999247.1 type IV pilus twitching motility protein PilT Machinery gene
  QMN23_RS10405 (GURASL_20550) - 2244784..2245998 (+) 1215 WP_281999248.1 type II secretion system F family protein -
  QMN23_RS10410 (GURASL_20560) - 2246004..2247677 (+) 1674 WP_281999249.1 ATP-binding protein -
  QMN23_RS10415 (GURASL_20570) - 2247679..2249058 (+) 1380 WP_281999250.1 sigma-54 dependent transcriptional regulator -
  QMN23_RS10420 (GURASL_20580) pilV 2249286..2249501 (+) 216 WP_281999251.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40655.04 Da        Isoelectric Point: 7.5398

>NTDB_id=99736 QMN23_RS10400 WP_281999247.1 2243623..2244717(+) (pilT) [Geotalea uraniireducens strain NIT-SL11]
MANIHQLLTELVNRGGSDLHVTTNTPPQIRIDGELIPLDMPPLNAIETKQLCYSILTEQQKHKFEENNELDLSFGIKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVVRDLAEKPRGLILVTGPTGSGKSTTLAAIIDKINTDRHEHIVTVE
DPIEYLHPHKNCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
IVDVFPSYQQPQVRAQLSFVLEGVMSQTLLPKASGKGRVLALEVMVPNPAIRNLIREDKIHQMYSQMQVGQEKFGMQTMN
QSLYSLLQKRLIALDVAMGRSPDPDELKQMLSSGVRPQQRPPMR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=99736 QMN23_RS10400 WP_281999247.1 2243623..2244717(+) (pilT) [Geotalea uraniireducens strain NIT-SL11]
ATGGCCAATATTCATCAACTGTTGACTGAGCTGGTCAATCGCGGGGGATCCGATCTCCACGTGACAACCAATACTCCGCC
GCAGATTCGTATCGATGGTGAGTTGATTCCCCTTGATATGCCACCTCTCAACGCAATAGAGACCAAGCAACTCTGTTACA
GTATCCTTACTGAGCAACAGAAGCACAAATTTGAGGAAAATAACGAACTCGATCTATCGTTCGGCATCAAAGGCCTCTCC
CGCTTCCGGGGTAATATCTTCGTCCAGCGGGGGGCCGTCGCCGGGGTTTTCCGGGTTATTCCCTACAAGATCCTCACATT
TGAAGAGCTTGGCTTGCCGCCAGTAGTCAGAGATCTGGCGGAGAAACCCCGCGGGCTGATTCTCGTTACCGGGCCGACCG
GCAGCGGGAAATCGACGACTCTGGCGGCCATTATCGATAAGATCAATACCGACCGGCATGAACATATCGTCACCGTTGAA
GACCCGATCGAATATCTGCATCCCCACAAGAATTGTGTCGTCAACCAGCGGGAAGTCGGTGCCGACACCAAAAGCTTCAA
AAATGCGCTCAAATACATCCTTCGCCAGGACCCGGATGTGGTCCTGGTAGGCGAGCTTCGCGACCTCGAGACGATTGAAG
CAGCACTGACCCTCGCCGAAACCGGCCATCTCTGCCTGGCGACCCTCCATACGAACTCGGCTGTGCAGACGATCAACAGG
ATTGTCGACGTATTCCCGTCCTATCAACAACCGCAGGTCCGTGCTCAGCTTTCGTTCGTGCTGGAAGGGGTGATGTCACA
GACGCTGTTACCGAAGGCTTCCGGCAAGGGACGAGTGCTGGCTCTTGAAGTCATGGTACCGAATCCGGCGATCCGCAACC
TCATCCGCGAGGACAAGATTCACCAGATGTACTCGCAAATGCAGGTTGGTCAGGAAAAATTCGGCATGCAGACGATGAAT
CAGTCGCTTTACTCGCTGCTTCAGAAACGTCTGATCGCCCTGGACGTGGCCATGGGCCGCTCACCGGACCCCGATGAACT
GAAACAGATGCTTTCCAGCGGCGTCCGGCCGCAGCAACGGCCACCGATGAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

55.522

92.033

0.511
  pilT Legionella pneumophila strain ERS1305867

55.522

92.033

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.299

95.604

0.5
  pilT Acinetobacter baylyi ADP1

52.174

94.78

0.495
  pilT Pseudomonas stutzeri DSM 10701

51.304

94.78

0.486
  pilT Pseudomonas aeruginosa PAK

51.014

94.78

0.484
  pilT Acinetobacter baumannii D1279779

50.725

94.78

0.481
  pilT Acinetobacter nosocomialis M2

50.725

94.78

0.481
  pilT Acinetobacter baumannii strain A118

50.725

94.78

0.481
  pilT Neisseria meningitidis 8013

48.266

95.055

0.459
  pilT Neisseria gonorrhoeae MS11

47.536

94.78

0.451
  pilT Vibrio cholerae strain A1552

49.394

90.659

0.448
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

90.659

0.448
  pilU Vibrio cholerae strain A1552

42.286

96.154

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.88

96.429

0.404
  pilU Acinetobacter baylyi ADP1

39.193

95.33

0.374

Multiple sequence alignment