Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAG092_RS13220 Genome accession   NZ_CP154874
Coordinates   2711169..2712203 (+) Length   344 a.a.
NCBI ID   WP_110682889.1    Uniprot ID   A0A2V4KTD5
Organism   Pseudomonas alcaligenes strain Med1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2706169..2717203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS13195 (AAG092_13125) - 2706548..2708536 (-) 1989 WP_373387031.1 dynamin family protein -
  AAG092_RS13200 (AAG092_13130) - 2708678..2708974 (-) 297 WP_373387032.1 DUF167 family protein -
  AAG092_RS13205 (AAG092_13135) - 2708971..2709561 (-) 591 WP_373387033.1 YggT family protein -
  AAG092_RS13210 (AAG092_13140) proC 2709571..2710389 (-) 819 WP_373387034.1 pyrroline-5-carboxylate reductase -
  AAG092_RS13215 (AAG092_13145) - 2710407..2711096 (-) 690 WP_373387035.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG092_RS13220 (AAG092_13150) pilT 2711169..2712203 (+) 1035 WP_110682889.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG092_RS13225 (AAG092_13155) pilU 2712256..2713401 (+) 1146 WP_373387036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG092_RS13230 (AAG092_13160) - 2713355..2714230 (-) 876 WP_373387037.1 TIGR03915 family putative DNA repair protein -
  AAG092_RS13235 (AAG092_13165) - 2714230..2715441 (-) 1212 WP_373387038.1 putative DNA modification/repair radical SAM protein -
  AAG092_RS13240 (AAG092_13170) - 2715591..2715989 (+) 399 WP_373387039.1 NINE protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38034.75 Da        Isoelectric Point: 6.8889

>NTDB_id=994333 AAG092_RS13220 WP_110682889.1 2711169..2712203(+) (pilT) [Pseudomonas alcaligenes strain Med1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFKKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDACL
KNLLSKGLVSREAAREKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=994333 AAG092_RS13220 WP_110682889.1 2711169..2712203(+) (pilT) [Pseudomonas alcaligenes strain Med1]
ATGGATATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGACCTGCACCTCTCGGCGGGTCTGCCGCCGAT
GATTCGCGTCGACGGCGACGTGCGGCGGATCAACCTGCCGCCGATGGACCACAAACAGGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGTGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTCCTGAGCATGGA
AGACCTCGGCATGGGCGAGGTGTTCAAGAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTGAACAACAACAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCTCCGA
GGCCCTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCGATGGTCCGCTCCATGCTCTCCGAATCCCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAAAAGATCGGCGGCGGCCGGGTGGCGGCCCACGAAATCATGATCGGCACTCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATTCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACGCCTGCCTG
AAGAACCTGCTGAGCAAGGGGCTGGTCAGCCGCGAGGCGGCGCGCGAGAAGGCCAAGCAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V4KTD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.349

100

0.953
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Vibrio cholerae strain A1552

39.823

98.547

0.392

Multiple sequence alignment