Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAG092_RS13225 Genome accession   NZ_CP154874
Coordinates   2712256..2713401 (+) Length   381 a.a.
NCBI ID   WP_373387036.1    Uniprot ID   -
Organism   Pseudomonas alcaligenes strain Med1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707256..2718401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS13200 (AAG092_13130) - 2708678..2708974 (-) 297 WP_373387032.1 DUF167 family protein -
  AAG092_RS13205 (AAG092_13135) - 2708971..2709561 (-) 591 WP_373387033.1 YggT family protein -
  AAG092_RS13210 (AAG092_13140) proC 2709571..2710389 (-) 819 WP_373387034.1 pyrroline-5-carboxylate reductase -
  AAG092_RS13215 (AAG092_13145) - 2710407..2711096 (-) 690 WP_373387035.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG092_RS13220 (AAG092_13150) pilT 2711169..2712203 (+) 1035 WP_110682889.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG092_RS13225 (AAG092_13155) pilU 2712256..2713401 (+) 1146 WP_373387036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG092_RS13230 (AAG092_13160) - 2713355..2714230 (-) 876 WP_373387037.1 TIGR03915 family putative DNA repair protein -
  AAG092_RS13235 (AAG092_13165) - 2714230..2715441 (-) 1212 WP_373387038.1 putative DNA modification/repair radical SAM protein -
  AAG092_RS13240 (AAG092_13170) - 2715591..2715989 (+) 399 WP_373387039.1 NINE protein -
  AAG092_RS13245 (AAG092_13175) - 2716047..2717318 (-) 1272 WP_373387040.1 dihydroorotase -
  AAG092_RS13250 (AAG092_13180) - 2717318..2718319 (-) 1002 WP_061903067.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42606.89 Da        Isoelectric Point: 6.8372

>NTDB_id=994334 AAG092_RS13225 WP_373387036.1 2712256..2713401(+) (pilU) [Pseudomonas alcaligenes strain Med1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVHSVMNEQQRREFAEKHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEDLKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNTSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLALATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTELGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=994334 AAG092_RS13225 WP_373387036.1 2712256..2713401(+) (pilU) [Pseudomonas alcaligenes strain Med1]
ATGGAATTCGAGAAACTGTTGCGCTTGATGGTGGAAAAGGGTGGTTCCGACCTGTTCATCACCGCCGGCGTGCCTCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGACCAAGAACCCCATGTCGCCGGAGCAGACCCGCGAGACGGTGCACTCGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCGCCGAGAAACACGAGTGCAACTTCGCCATCAGTGCCCGTGGCATCGGCCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGCTGGCATGGTGCTGCGCCGCATCGAGACCAATATCCCGACCATTGA
GGACCTCAAACTGCCCGAGGTGCTGAAGAAGCTGGCACTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACCTCGCTAGCGGCGATGATCGGCTACCGCAACAAGAACACCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAACGCGAGGTCGGCATCGATACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGGACCCGCGAGACCATGGATTACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGGCCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAAGTGTGGATGGACCTTTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACCCCGGACGGCAAGGGCCGGCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAAGGCGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCC
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGCTGTATGCCGACTCGGCCAACGACCTGCGCCT
GCTGATCAAGCTGGGCTCGGAAACCGATGGCGAGCACCTCACCAGCGTGAGCCAGGGTCTCAGTCTGGAGGTGAGCGAGG
AAGATCCGGGGCGGCGTTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

93.176

100

0.932
  pilU Acinetobacter baylyi ADP1

64.706

93.701

0.606
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

88.189

0.386

Multiple sequence alignment