Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAJG31_RS07630 Genome accession   NZ_CP154860
Coordinates   1460590..1461636 (-) Length   348 a.a.
NCBI ID   WP_279494297.1    Uniprot ID   -
Organism   Lysinibacillus sp. BS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1455590..1466636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAJG31_RS07600 (AAJG31_07600) - 1455890..1456582 (-) 693 WP_054550589.1 hypothetical protein -
  AAJG31_RS07605 (AAJG31_07605) - 1456579..1457172 (-) 594 WP_066036586.1 hypothetical protein -
  AAJG31_RS07610 (AAJG31_07610) pilM 1457172..1458146 (-) 975 WP_066036585.1 pilus assembly protein PilM -
  AAJG31_RS07615 (AAJG31_07615) - 1458259..1458729 (-) 471 WP_279494301.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAJG31_RS07620 (AAJG31_07620) - 1458786..1459262 (-) 477 WP_279494300.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAJG31_RS07625 (AAJG31_07625) - 1459388..1460593 (-) 1206 WP_036080216.1 type II secretion system F family protein -
  AAJG31_RS07630 (AAJG31_07630) pilT 1460590..1461636 (-) 1047 WP_279494297.1 type IV pilus twitching motility protein PilT Machinery gene
  AAJG31_RS07635 (AAJG31_07635) - 1461648..1463312 (-) 1665 WP_279496428.1 GspE/PulE family protein -
  AAJG31_RS07640 (AAJG31_07640) - 1463336..1464667 (-) 1332 WP_279494295.1 G5 domain-containing protein -
  AAJG31_RS07645 (AAJG31_07645) - 1465040..1465465 (+) 426 WP_279494293.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAJG31_RS07650 (AAJG31_07650) - 1465471..1465914 (+) 444 WP_279494292.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38901.70 Da        Isoelectric Point: 6.7861

>NTDB_id=994170 AAJG31_RS07630 WP_279494297.1 1460590..1461636(-) (pilT) [Lysinibacillus sp. BS3]
MTAYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGTVPLETEDLKDMVKVILPVHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAEIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDHINETRSKHIIT
LEDPIEYLHTHKKSVINQREIGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFVRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
EYSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=994170 AAJG31_RS07630 WP_279494297.1 1460590..1461636(-) (pilT) [Lysinibacillus sp. BS3]
ATGACAGCATATTCAATCGTTGAATTATTAACACGTGCATTTGAAGAGAAAGCATCGGATCTACATTTAACAAAAGGTAT
TCCACCAGTATATCGTATTCATGGGCAACTGGAGCATTATGGCACAGTGCCGTTAGAAACAGAAGATTTAAAGGATATGG
TAAAGGTCATTCTCCCTGTCCATAAACAACAGGAGTTTGAGGACAAAGGAGAAACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTCAACGCCTATCATCAGCGAAATGAAGTAACGATTGCGGCTCGTTTAATTGAGGCTGAAATTCC
TTCTATTGAAGCGCTCAATATGCCACAGGTGTTATATAAGCTTGCGGAAAAACCACAAGGCTTAATTTTAGTGACTGGCC
CAACAGGCTCAGGAAAATCAACTACCTTGGCAGCAATGATTGATCATATTAATGAAACGAGATCAAAACATATTATTACG
TTAGAGGATCCAATCGAATACTTGCATACACATAAGAAATCTGTCATCAATCAGCGTGAGATTGGTGTCGATACGATGAG
CTTTGCAAATGGTTTACGTGCATCACTACGTCAGGACCCAGATATTATTTTAGTCGGGGAGATGCGAGATTACGAAACCA
TTTCAACGGCTATTACGGCTGCTGAAACAGGGCATTTAGTCATGGCTACACTTCATACAAGTAGTGCACCAACAACTATC
GACCGTATTATAGACGTATTTCCACCACATCAGCAAAGTCAAATTCGTGTGCAGCTTGCAAATGTACTGCAAGGTATTGT
TTCTCAGCGTTTATTTGTACGTAAGGATGTAAAAGGGCGTATTGCGGCAACAGAAATTTTAGTACAGGCACCTGCCATCT
CGAATTTAATTCGTAATGAAAAAATCCATCAAATTCCAAGTGTGATGCAGACAAGTAAAGCATTAGGTATGCACACGTTG
GAATATTCGATGCAACACTTAATTACGACAGGTAAGATTTCACTTGAGGATGCACGACCATATATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.083

96.552

0.503
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

49.701

95.977

0.477
  pilT Acinetobacter baumannii D1279779

49.102

95.977

0.471
  pilT Acinetobacter nosocomialis M2

49.102

95.977

0.471
  pilT Acinetobacter baumannii strain A118

49.102

95.977

0.471
  pilT Legionella pneumophila strain Lp02

49.544

94.54

0.468
  pilT Legionella pneumophila strain ERS1305867

49.544

94.54

0.468
  pilT Pseudomonas aeruginosa PAK

48.802

95.977

0.468
  pilT Vibrio cholerae strain A1552

48.503

95.977

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

95.977

0.466
  pilT Acinetobacter baylyi ADP1

47.904

95.977

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.904

95.977

0.46
  pilU Pseudomonas stutzeri DSM 10701

42.296

95.115

0.402
  pilU Vibrio cholerae strain A1552

42.236

92.529

0.391
  pilU Acinetobacter baylyi ADP1

39.759

95.402

0.379

Multiple sequence alignment