Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAG895_RS17020 Genome accession   NZ_CP154859
Coordinates   3744706..3745749 (+) Length   347 a.a.
NCBI ID   WP_345793162.1    Uniprot ID   -
Organism   Thauera sp. JM12B12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3739706..3750749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG895_RS16995 (AAG895_16995) - 3740093..3741052 (+) 960 WP_345793157.1 aspartate carbamoyltransferase catalytic subunit -
  AAG895_RS17000 (AAG895_17000) - 3741085..3742362 (+) 1278 WP_345793158.1 dihydroorotase -
  AAG895_RS17005 (AAG895_17005) - 3742453..3743034 (-) 582 WP_345793159.1 YggT family protein -
  AAG895_RS17010 (AAG895_17010) proC 3743038..3743862 (-) 825 WP_345793160.1 pyrroline-5-carboxylate reductase -
  AAG895_RS17015 (AAG895_17015) - 3743906..3744613 (-) 708 WP_345793161.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG895_RS17020 (AAG895_17020) pilT 3744706..3745749 (+) 1044 WP_345793162.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG895_RS17025 (AAG895_17025) pilU 3745769..3746902 (+) 1134 WP_345793163.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG895_RS17030 (AAG895_17030) - 3746928..3748091 (-) 1164 WP_345793164.1 THUMP domain-containing protein -
  AAG895_RS17035 (AAG895_17035) - 3748373..3749962 (+) 1590 WP_345793165.1 FecR domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38680.39 Da        Isoelectric Point: 7.0158

>NTDB_id=994161 AAG895_RS17020 WP_345793162.1 3744706..3745749(+) (pilT) [Thauera sp. JM12B12]
MDITELLAFAVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKEVHAMVYDIMNDAQRKQYEESWECDFSFAVPNLAR
FRVNAFNQNRGSAAVFRTIPSKVLTLEELNCPKIFKEIANQPRGIVLVTGPTGSGKSTTLAAMIDYVNENEYGHILTVED
PIEFVHESKRCLINQREVHRDTMSFNNALRAALREDPDVVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNMGMQTLDQC
LADLVRRNVVSAAEARMKAQNKDNFAA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=994161 AAG895_RS17020 WP_345793162.1 3744706..3745749(+) (pilT) [Thauera sp. JM12B12]
ATGGACATCACCGAACTGCTTGCCTTCGCGGTCAAGAACAAGGCCTCCGACCTGCATCTGTCGGCGGGGCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAACCTGCCGCCGCTCGAGCACAAGGAAGTGCACGCGATGGTGTACGACA
TCATGAACGACGCCCAGCGCAAGCAGTACGAGGAGAGCTGGGAGTGCGACTTCTCCTTCGCCGTGCCCAACCTCGCCCGC
TTCCGTGTCAACGCCTTCAACCAGAACCGGGGCTCGGCGGCGGTGTTCCGGACGATTCCGTCCAAGGTGCTGACGCTGGA
GGAGCTCAACTGCCCGAAGATCTTCAAGGAGATCGCCAACCAGCCGCGCGGCATCGTGCTCGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACGTCAATGAGAACGAGTACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCATGAATCCAAGCGCTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGATGTCCTTCAACAA
CGCCCTGCGCGCCGCGCTGCGCGAGGACCCGGACGTGGTGCTGGTGGGCGAGATGCGCGACCTCGAGACCATCCGCCTCG
CGCTCACCGCAGCCGAGACCGGCCACCTCGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCTGCGGCCGAGAAGGACATGGTGCGCTCGATGCTCTCGGAATCCCTGCGCGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGATCGGCACCCCCGCGATTCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCCTCGATCCAGACCGGCCAGAACATGGGCATGCAGACGCTCGACCAGTGC
CTCGCCGACCTCGTGCGCCGCAACGTGGTGTCGGCGGCCGAGGCGCGCATGAAGGCGCAGAACAAGGACAATTTCGCGGC
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Legionella pneumophila strain Lp02

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.913

99.424

0.735
  pilT Neisseria meningitidis 8013

71.098

99.712

0.709
  pilT Neisseria gonorrhoeae MS11

70.809

99.712

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.118

97.983

0.677
  pilT Vibrio cholerae strain A1552

69.118

97.983

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.89

99.712

0.527
  pilU Acinetobacter baylyi ADP1

41.954

100

0.421
  pilU Vibrio cholerae strain A1552

40.87

99.424

0.406
  pilU Pseudomonas stutzeri DSM 10701

40

99.424

0.398

Multiple sequence alignment