Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAG895_RS17025 Genome accession   NZ_CP154859
Coordinates   3745769..3746902 (+) Length   377 a.a.
NCBI ID   WP_345793163.1    Uniprot ID   -
Organism   Thauera sp. JM12B12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3740769..3751902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG895_RS17000 (AAG895_17000) - 3741085..3742362 (+) 1278 WP_345793158.1 dihydroorotase -
  AAG895_RS17005 (AAG895_17005) - 3742453..3743034 (-) 582 WP_345793159.1 YggT family protein -
  AAG895_RS17010 (AAG895_17010) proC 3743038..3743862 (-) 825 WP_345793160.1 pyrroline-5-carboxylate reductase -
  AAG895_RS17015 (AAG895_17015) - 3743906..3744613 (-) 708 WP_345793161.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG895_RS17020 (AAG895_17020) pilT 3744706..3745749 (+) 1044 WP_345793162.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG895_RS17025 (AAG895_17025) pilU 3745769..3746902 (+) 1134 WP_345793163.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG895_RS17030 (AAG895_17030) - 3746928..3748091 (-) 1164 WP_345793164.1 THUMP domain-containing protein -
  AAG895_RS17035 (AAG895_17035) - 3748373..3749962 (+) 1590 WP_345793165.1 FecR domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42216.62 Da        Isoelectric Point: 6.6637

>NTDB_id=994162 AAG895_RS17025 WP_345793163.1 3745769..3746902(+) (pilU) [Thauera sp. JM12B12]
MERDQALKFMHDLLRLMLQKNGSDLFITSGFPPAIKIDGKIVPQSNQMLQAQHTAELARAIMNDRQAADFEAHKECNFGI
SPAGIGRFRANAFMQQGKVGLVLRTIPQKIPNFGELGLPPVLNDIALAKRGLVIFVGGTGTGKTTSLASMVDYRNENTFG
HIITIEDPIEFLHPHKNCIVTQREVGIDTESWEAALKNTLRQAPDVILMGEIRDRETMDYAVAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMISQRLLPLKDRKGRVPAVEVLLNSPLIADLIFKGNVPEIKEIMKRSRELG
MQTFDQSLFQLYEDELITYEDALRNADSVNDLRLQIKLHSRHGEQDSSGGLKNLGIL

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=994162 AAG895_RS17025 WP_345793163.1 3745769..3746902(+) (pilU) [Thauera sp. JM12B12]
ATGGAACGCGATCAGGCCCTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCTCCGGCTTTCCACCCGCGATCAAGATCGACGGCAAGATCGTGCCGCAGTCGAACCAGATGCTGCAGGCGCAGCACA
CCGCCGAGCTCGCGCGCGCGATCATGAACGACCGCCAGGCAGCCGACTTCGAGGCGCACAAGGAGTGCAACTTCGGCATC
TCGCCGGCCGGCATCGGCCGCTTCCGCGCCAACGCCTTCATGCAGCAGGGCAAGGTCGGGCTGGTGCTGCGCACGATCCC
GCAGAAGATTCCCAACTTCGGTGAGCTCGGCCTGCCGCCGGTGCTCAACGACATCGCGCTCGCCAAGCGCGGCCTGGTGA
TCTTCGTGGGCGGCACCGGTACCGGCAAGACGACCTCGCTCGCGTCGATGGTCGACTACCGCAACGAAAACACCTTCGGC
CACATCATCACGATCGAGGACCCGATCGAGTTCCTCCACCCGCACAAGAACTGCATCGTCACCCAGCGCGAGGTCGGCAT
CGACACCGAATCCTGGGAGGCCGCGCTCAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCCGTCGCCTTCGCCGAGACCGGCCACCTGTGCCTAGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAGCTGCTGATGGACCTCTCGCTCAACCT
GCGCGCGATGATCTCTCAGCGCCTGCTGCCGCTGAAGGACCGCAAGGGTCGCGTGCCCGCGGTCGAGGTGCTGCTCAACT
CCCCGCTGATCGCCGACCTGATCTTCAAGGGCAACGTGCCCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGATCAGTCGCTGTTCCAGCTCTACGAGGACGAATTGATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCCACAGCCGTCATGGCGAGCAGGACAGCAGCGGCGGGCTGAAGAACC
TCGGCATCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.655

92.308

0.597
  pilU Acinetobacter baylyi ADP1

59.195

92.308

0.546
  pilU Vibrio cholerae strain A1552

55.278

95.491

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.463

88.859

0.422
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.859

0.374
  pilT Acinetobacter baylyi ADP1

41.84

89.39

0.374
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Acinetobacter nosocomialis M2

41.692

87.798

0.366
  pilT Acinetobacter baumannii D1279779

41.692

87.798

0.366
  pilT Acinetobacter baumannii strain A118

41.692

87.798

0.366
  pilT Legionella pneumophila strain ERS1305867

41.39

87.798

0.363
  pilT Legionella pneumophila strain Lp02

41.39

87.798

0.363

Multiple sequence alignment