Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAIA72_RS13560 Genome accession   NZ_CP154858
Coordinates   2804139..2805173 (+) Length   344 a.a.
NCBI ID   WP_369600837.1    Uniprot ID   A0AB39UU47
Organism   Hahella sp. SMD15-11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2799139..2810173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA72_RS13535 (AAIA72_13500) - 2799488..2801458 (-) 1971 WP_369600832.1 dynamin family protein -
  AAIA72_RS13540 (AAIA72_13505) - 2801550..2801846 (-) 297 WP_369600833.1 DUF167 family protein -
  AAIA72_RS13545 (AAIA72_13510) - 2801856..2802416 (-) 561 WP_369600834.1 YggT family protein -
  AAIA72_RS13550 (AAIA72_13515) proC 2802488..2803327 (-) 840 WP_369600835.1 pyrroline-5-carboxylate reductase -
  AAIA72_RS13555 (AAIA72_13520) - 2803324..2804034 (-) 711 WP_369600836.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAIA72_RS13560 (AAIA72_13525) pilT 2804139..2805173 (+) 1035 WP_369600837.1 type IV pilus twitching motility protein PilT Machinery gene
  AAIA72_RS13565 (AAIA72_13530) pilU 2805200..2806327 (+) 1128 WP_369600838.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAIA72_RS13570 (AAIA72_13535) - 2806367..2806903 (-) 537 WP_369600839.1 hypothetical protein -
  AAIA72_RS13575 (AAIA72_13540) - 2807017..2807709 (+) 693 WP_369600840.1 GntR family transcriptional regulator -
  AAIA72_RS13580 (AAIA72_13545) - 2807808..2808080 (+) 273 WP_369600841.1 hypothetical protein -
  AAIA72_RS13585 (AAIA72_13550) - 2808083..2808355 (-) 273 WP_369600842.1 acyl-CoA-binding protein -
  AAIA72_RS13590 (AAIA72_13555) - 2808468..2809637 (-) 1170 WP_369600843.1 translocation/assembly module TamB domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38018.79 Da        Isoelectric Point: 6.3488

>NTDB_id=994138 AAIA72_RS13560 WP_369600837.1 2804139..2805173(+) (pilT) [Hahella sp. SMD15-11]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKEVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGQVFKDIALKPRGLVLVTGPTGSGKSTTLAAMIDFINETRYDHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKEMVRSMLSESLQAVVSQTLLKKNGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDQCL
QNLVAKGIISRDVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=994138 AAIA72_RS13560 WP_369600837.1 2804139..2805173(+) (pilT) [Hahella sp. SMD15-11]
ATGGATATTACTGAACTGCTTGCGTTTTCAGCCAAACAGGGCGCTTCCGACCTTCACCTCTCTGCCGGTCTGCCACCGAT
GATCCGTGTGGACGGCGACGTGCGTCGGATCAACCTGCCGCCACTGGAGCACAAGGAAGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTATGAAGAGTTTCTGGAGACGGACTTCTCCTTCGAGGTGCCGGGTGTGGCGCGT
TTCCGGGTCAATGCCTTCAACCACAACCGGGGTGCGGGGGCCGTGTTCCGGACCATTCCTTCCAAGGTGCTGACCATGGA
AGATCTGGGCATGGGACAGGTGTTCAAGGATATTGCCCTCAAGCCGCGTGGTCTGGTGCTGGTCACGGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGACTTCATCAACGAGACCCGTTACGACCATATCCTGACCATCGAGGAC
CCCATCGAATTCGTGCACGAATCCAAGAAGTGTCTGGTCAACCAGCGCGAAGTGCACCGGGATACGCTGGGTTTTGCCGA
GGCACTGCGTTCGGCCCTGCGGGAAGACCCGGACATCATTCTGGTCGGTGAGATGCGGGATCTGGAAACCATTCGTCTGG
CCCTGACGGCGGCGGAAACCGGTCACCTGGTATTCGGTACCCTGCACACCACCTCGGCGGCCAAGACCATTGACCGGGTG
GTGGACGTGTTCCCGGCGGCGGAGAAGGAGATGGTGCGCTCCATGCTCTCCGAGTCCCTGCAGGCGGTCGTTTCACAGAC
ACTGCTGAAGAAGAACGGTGGCGGTCGCGTGGCGGCTCACGAAATCATGATCGGTACGCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATTCAGACCGGCGGTTCGCTGGGCATGCAGACGCTGGACCAGTGCCTG
CAGAACCTGGTGGCCAAGGGCATCATTTCCCGTGATGTGGCCCGCGAGAAGGCCAAGATGCCCGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

91.279

100

0.913
  pilT Pseudomonas stutzeri DSM 10701

90.407

100

0.904
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

77.219

98.256

0.759
  pilT Legionella pneumophila strain ERS1305867

77.219

98.256

0.759
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.696

100

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.059

98.837

0.663
  pilT Vibrio cholerae strain A1552

67.059

98.837

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

41.39

96.221

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment