Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMG15_RS00895 Genome accession   NZ_AP027055
Coordinates   173315..174319 (-) Length   334 a.a.
NCBI ID   WP_281790051.1    Uniprot ID   -
Organism   Limnohabitans sp. INBF002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168315..179319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG15_RS00870 (LINBF2_01740) rsmI 169631..170542 (-) 912 WP_281789061.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  QMG15_RS00875 (LINBF2_01750) - 170555..170965 (+) 411 WP_281789062.1 YraN family protein -
  QMG15_RS00880 (LINBF2_01760) - 170981..171580 (+) 600 WP_108360264.1 phosphoheptose isomerase -
  QMG15_RS00885 (LINBF2_01770) - 171577..172257 (+) 681 WP_108360263.1 BON domain-containing protein -
  QMG15_RS00890 (LINBF2_01780) - 172315..173229 (-) 915 WP_281789063.1 NAD(P)-dependent oxidoreductase -
  QMG15_RS00895 (LINBF2_01790) pilT 173315..174319 (-) 1005 WP_281790051.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG15_RS00900 (LINBF2_01800) - 174365..175081 (+) 717 WP_281789064.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMG15_RS00905 (LINBF2_01810) - 175094..176596 (-) 1503 WP_281789065.1 FAD-linked oxidase C-terminal domain-containing protein -
  QMG15_RS00910 (LINBF2_01820) - 176764..177639 (-) 876 WP_281789066.1 LysR substrate-binding domain-containing protein -
  QMG15_RS00915 (LINBF2_01830) purU 177646..178491 (-) 846 WP_281789067.1 formyltetrahydrofolate deformylase -
  QMG15_RS00920 (LINBF2_01840) - 178564..178842 (-) 279 WP_108360301.1 DUF3567 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36010.14 Da        Isoelectric Point: 6.6668

>NTDB_id=99407 QMG15_RS00895 WP_281790051.1 173315..174319(-) (pilT) [Limnohabitans sp. INBF002]
MTLNELLTQAVAQHASDLHLSAGLAPQVRAQGVLTPLQGPTTSHPLAHEALQRMLTDLMDEAQHTQFMQGHEVDFAFAVP
ELGRFRANAFMQQHGCGTVMRHIPSGTPTLAALHAPRVLPDLLKQVGLLLVTGPTGSGKSTTLAAMVQHLNTSTQQHLVT
LEDPIEFVHTSDQCLITQREIGTHSHSFAHALRAALREDPDVILVGELRDLETIRLALTAAETGHLVLASLHTRSAAATV
ERMVDVFDAHEKALVRTQLSDALVGVVSQTLCPTPTKDGRVAVFETLVATPAIRHLIREGKTAQMQSTQQTGAAYGMQSM
AQALQDARHQGLIS

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=99407 QMG15_RS00895 WP_281790051.1 173315..174319(-) (pilT) [Limnohabitans sp. INBF002]
CTGACTTTGAATGAACTCCTCACCCAAGCGGTGGCGCAACACGCGTCTGATTTACACCTGTCGGCAGGGTTGGCGCCCCA
GGTGCGCGCGCAAGGCGTGTTGACGCCGCTGCAAGGCCCCACCACGTCTCACCCGCTCGCGCATGAAGCGCTACAAAGAA
TGTTGACTGACTTGATGGACGAAGCGCAACACACGCAATTCATGCAAGGCCATGAAGTTGACTTCGCATTTGCCGTGCCC
GAGCTGGGGCGTTTTCGGGCGAATGCCTTCATGCAACAACACGGCTGCGGCACGGTGATGCGCCACATTCCCAGCGGCAC
GCCCACGCTGGCGGCGTTGCACGCACCGCGTGTGCTGCCGGACTTGCTCAAGCAAGTTGGTTTGCTGTTGGTGACAGGGC
CAACGGGCTCTGGCAAATCCACCACCTTGGCCGCGATGGTGCAACACCTGAACACCAGCACGCAGCAACACCTCGTGACG
CTGGAAGACCCGATTGAGTTTGTGCACACCAGCGATCAATGCCTGATTACCCAGCGCGAAATTGGCACGCATAGCCACAG
CTTTGCGCACGCGTTACGCGCCGCCTTGCGCGAAGACCCCGACGTGATTTTGGTGGGTGAGCTGCGCGACCTAGAAACCA
TTCGCCTCGCCCTCACAGCCGCCGAAACCGGGCACTTGGTGCTGGCCAGCCTGCACACGCGCAGCGCGGCGGCCACGGTC
GAGCGCATGGTGGATGTGTTTGACGCACACGAAAAAGCCTTGGTACGCACCCAACTCAGCGATGCGTTGGTGGGCGTGGT
GTCGCAAACACTCTGCCCCACACCAACGAAAGATGGCCGCGTGGCGGTGTTTGAAACGCTGGTGGCCACCCCCGCCATTC
GTCACCTGATTCGCGAGGGCAAAACCGCACAGATGCAAAGCACCCAGCAAACAGGTGCCGCGTACGGCATGCAAAGCATG
GCCCAAGCGCTTCAAGACGCCCGCCACCAAGGACTGATCTCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.507

100

0.533
  pilT Vibrio cholerae strain A1552

52.994

100

0.53
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.994

100

0.53
  pilT Pseudomonas stutzeri DSM 10701

51.929

100

0.524
  pilT Acinetobacter baylyi ADP1

51.343

100

0.515
  pilT Acinetobacter baumannii strain A118

51.343

100

0.515
  pilT Acinetobacter nosocomialis M2

51.343

100

0.515
  pilT Acinetobacter baumannii D1279779

51.343

100

0.515
  pilT Neisseria meningitidis 8013

51.343

100

0.515
  pilT Neisseria gonorrhoeae MS11

51.045

100

0.512
  pilT Legionella pneumophila strain Lp02

49.851

100

0.5
  pilT Legionella pneumophila strain ERS1305867

49.851

100

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.975

96.108

0.461
  pilU Pseudomonas stutzeri DSM 10701

39.403

100

0.395
  pilU Vibrio cholerae strain A1552

40

97.305

0.389
  pilU Acinetobacter baylyi ADP1

38.72

98.204

0.38

Multiple sequence alignment