Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAEQ75_RS06345 Genome accession   NZ_CP154631
Coordinates   1322772..1323806 (-) Length   344 a.a.
NCBI ID   WP_125879700.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain I4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1317772..1328806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEQ75_RS06325 (AAEQ75_06325) - 1318889..1319287 (-) 399 WP_099525928.1 TM2 domain-containing protein -
  AAEQ75_RS06330 (AAEQ75_06330) - 1319436..1320647 (+) 1212 WP_125879696.1 putative DNA modification/repair radical SAM protein -
  AAEQ75_RS06335 (AAEQ75_06335) - 1320647..1321534 (+) 888 WP_125879698.1 TIGR03915 family putative DNA repair protein -
  AAEQ75_RS06340 (AAEQ75_06340) pilU 1321500..1322645 (-) 1146 WP_099525931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAEQ75_RS06345 (AAEQ75_06345) pilT 1322772..1323806 (-) 1035 WP_125879700.1 type IV pilus twitching motility protein PilT Machinery gene
  AAEQ75_RS06350 (AAEQ75_06350) - 1323878..1324570 (+) 693 WP_125879702.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAEQ75_RS06355 (AAEQ75_06355) proC 1324588..1325406 (+) 819 WP_125879703.1 pyrroline-5-carboxylate reductase -
  AAEQ75_RS06360 (AAEQ75_06360) - 1325418..1326011 (+) 594 WP_003463941.1 YggT family protein -
  AAEQ75_RS06365 (AAEQ75_06365) - 1326093..1326398 (+) 306 WP_218893249.1 DUF167 domain-containing protein -
  AAEQ75_RS06370 (AAEQ75_06370) - 1326580..1328556 (+) 1977 WP_343351187.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37961.64 Da        Isoelectric Point: 6.8890

>NTDB_id=993848 AAEQ75_RS06345 WP_125879700.1 1322772..1323806(-) (pilT) [Pseudomonas sediminis strain I4]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSRGLVSRDSAREKAKSPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=993848 AAEQ75_RS06345 WP_125879700.1 1322772..1323806(-) (pilT) [Pseudomonas sediminis strain I4]
ATGGATATCACTGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCGGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTACGCCGCATCAACCTGCCGCCGCTGGATCACAAGCAGGTACACGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGACTTCGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGTGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTGGGCATGGGCGAGGTGTTTCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTACCACCACATTCTCACTATCGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCGATGGTGCGTTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGTGTTGCTGCTCACGAAATCATGATCGGTACGCCGGCCATCCGTAACCTGATCC
GTGAGGACAAGGTCGCGCAAATGTATTCAGCGATCCAGACCGGTGGCGCGCTGGGCATGCAGACCCTCGATGCCTGTCTG
AAGACGTTGGTGTCCAGGGGACTGGTCAGCCGCGACAGTGCGCGCGAGAAGGCCAAGAGCCCGGAAAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment