Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EQB46_RS00385 Genome accession   NZ_LR129843
Coordinates   59272..60633 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain 180-2 isolate 180-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 54272..65633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB46_RS12035 - 54294..54410 (+) 117 WP_001063632.1 hypothetical protein -
  EQB46_RS00335 - 54391..54594 (+) 204 WP_001091113.1 hypothetical protein -
  EQB46_RS00340 - 54597..54947 (+) 351 WP_000852241.1 hypothetical protein -
  EQB46_RS00345 - 54956..55372 (+) 417 WP_001165344.1 phage holin family protein -
  EQB46_RS00350 - 55376..55708 (+) 333 WP_001186219.1 phage holin -
  EQB46_RS00355 - 55712..56668 (+) 957 WP_000350505.1 N-acetylmuramoyl-L-alanine amidase -
  EQB46_RS00360 - 56889..57077 (-) 189 WP_000109850.1 hypothetical protein -
  EQB46_RS00365 tadA 57645..58112 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  EQB46_RS00375 - 58298..58741 (+) 444 WP_000701992.1 dUTP diphosphatase -
  EQB46_RS00380 - 58743..59258 (+) 516 WP_001838385.1 histidine phosphatase family protein -
  EQB46_RS00385 radA 59272..60633 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  EQB46_RS00390 - 60706..61203 (+) 498 WP_001809263.1 beta-class carbonic anhydrase -
  EQB46_RS00400 - 61228..62058 (+) 831 Protein_72 PrsW family glutamic-type intramembrane protease -
  EQB46_RS00405 - 62203..63171 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  EQB46_RS00410 - 63308..63586 (-) 279 Protein_74 transposase family protein -
  EQB46_RS11555 - 63630..64555 (-) 926 Protein_75 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=993847 EQB46_RS00385 WP_074017595.1 59272..60633(+) (radA) [Streptococcus pneumoniae strain 180-2 isolate 180-2]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=993847 EQB46_RS00385 WP_074017595.1 59272..60633(+) (radA) [Streptococcus pneumoniae strain 180-2 isolate 180-2]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTTGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTACA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA/sms Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment