Detailed information NTG0993847

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAEQ75_RS06340
• Genome accession: NZ_CP154631
• Coordinates: 1321500..1322645 (-)
• Length: 381 a.a.
• NCBI ID: WP_099525931.1
• Uniprot ID: -
• Organism: Pseudomonas sediminis strain I4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316500..1327645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEQ75_RS06320 (AAEQ75_06320)	ntrC	1317362..1318795 (+)	1434	WP_343351185.1	nitrogen regulation protein NR(I)	-
AAEQ75_RS06325 (AAEQ75_06325)	-	1318889..1319287 (-)	399	WP_099525928.1	TM2 domain-containing protein	-
AAEQ75_RS06330 (AAEQ75_06330)	-	1319436..1320647 (+)	1212	WP_125879696.1	putative DNA modification/repair radical SAM protein	-
AAEQ75_RS06335 (AAEQ75_06335)	-	1320647..1321534 (+)	888	WP_125879698.1	TIGR03915 family putative DNA repair protein	-
AAEQ75_RS06340 (AAEQ75_06340)	pilU	1321500..1322645 (-)	1146	WP_099525931.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAEQ75_RS06345 (AAEQ75_06345)	pilT	1322772..1323806 (-)	1035	WP_125879700.1	type IV pilus twitching motility protein PilT	Machinery gene
AAEQ75_RS06350 (AAEQ75_06350)	-	1323878..1324570 (+)	693	WP_125879702.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAEQ75_RS06355 (AAEQ75_06355)	proC	1324588..1325406 (+)	819	WP_125879703.1	pyrroline-5-carboxylate reductase	-
AAEQ75_RS06360 (AAEQ75_06360)	-	1325418..1326011 (+)	594	WP_003463941.1	YggT family protein	-
AAEQ75_RS06365 (AAEQ75_06365)	-	1326093..1326398 (+)	306	WP_218893249.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42447.75 Da
• Isoelectric Point: 6.5934

>NTDB_id=993847 AAEQ75_RS06340 WP_099525931.1 1321500..1322645(-) (pilU) [Pseudomonas sediminis strain I4]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETTIPTIDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNRNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAQGLALEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=993847 AAEQ75_RS06340 WP_099525931.1 1321500..1322645(-) (pilU) [Pseudomonas sediminis strain I4]
ATGGAATTCGAAAAACTACTGCGCCTGATGGTCGAAAAAGGCGGCTCCGACCTGTTCATCACCGCTGGCGTACCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCGGTGACCAAGAACGCCATGTCGCCGGAGCAGACCCGCGAGACCGTATTGGGCG
TGATGAACGAGGCACAGCGTCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGTGGTATCGGTCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGTTGCGCCGGATCGAGACCACCATCCCGACCATCGA
CGAACTCAAGCTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACGGGTA
CCGGCAAGTCGACCTCTCTGGCGGCGATGATCGGCTACCGCAACAGGAACAGCAGCGGCCATATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAATCGTTCGAAGT
GGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGGACCCGCGAGACCATGGATCATG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCTCAGCA
GTTGATTCCAACTCCAGATGGTAAAGGTCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCTGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAGGGTTTGATGAAGCGTTCCACCGAACAGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAAGATGCGCTGCTCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGATCGGAAACCGACGGCGATCACCTGACCAGCATGGCTCAGGGTCTGGCGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	59.143	91.864	0.543
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.048	88.189	0.388