Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAHN93_RS02240 Genome accession   NZ_CP154474
Coordinates   484752..485795 (+) Length   347 a.a.
NCBI ID   WP_095542791.1    Uniprot ID   A0A2A2ATU7
Organism   Vandammella animalimorsus strain W29-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479752..490795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHN93_RS02220 (AAHN93_02220) - 480130..480828 (-) 699 WP_343293272.1 hypothetical protein -
  AAHN93_RS02225 (AAHN93_02225) - 481508..482101 (+) 594 WP_343293273.1 nitroreductase family protein -
  AAHN93_RS02230 (AAHN93_02230) - 482119..483117 (+) 999 WP_343293274.1 LD-carboxypeptidase -
  AAHN93_RS02235 (AAHN93_02235) - 483502..484458 (+) 957 WP_343293275.1 tripartite tricarboxylate transporter substrate-binding protein -
  AAHN93_RS02240 (AAHN93_02240) pilT 484752..485795 (+) 1044 WP_095542791.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHN93_RS02245 (AAHN93_02245) - 485862..486512 (+) 651 WP_124858275.1 cyclic nucleotide-binding domain-containing protein -
  AAHN93_RS02250 (AAHN93_02250) pilU 486582..487718 (+) 1137 WP_095550180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAHN93_RS02255 (AAHN93_02255) - 487789..488721 (+) 933 WP_095542794.1 NAD(P)-dependent oxidoreductase -
  AAHN93_RS02260 (AAHN93_02260) aroQ 488987..489466 (+) 480 WP_095542795.1 type II 3-dehydroquinate dehydratase -
  AAHN93_RS02265 (AAHN93_02265) - 489587..490228 (+) 642 WP_095542796.1 DUF6806 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38520.29 Da        Isoelectric Point: 6.7785

>NTDB_id=993553 AAHN93_RS02240 WP_095542791.1 484752..485795(+) (pilT) [Vandammella animalimorsus strain W29-2]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVEPLDHRTVFSMVYDIMSDAQRKTYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKVLTLEQLNAPRIFADLALKPRGLILVTGPTGSGKSTTLAAMINHLNESEYGHILTVED
PIEFVHESKKCLINQREVGPMTHSFTAALRSALREDPDAILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKEMVRSMLSESLEAVISQTLLKTKDGSSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNQGMQTLDQN
LVELVRRNVISARDARLKAKMPENFPV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=993553 AAHN93_RS02240 WP_095542791.1 484752..485795(+) (pilT) [Vandammella animalimorsus strain W29-2]
GTGGACATCACCCAATTGCTGGCCTTCAGCGTCAAGAACAAGGCATCGGACTTGCACCTCTCGGCGGGCCTGCCGCCGAT
GATTCGCGTCAACGGTGACGTGCGACGCATCAACGTCGAGCCGCTGGATCACCGCACGGTGTTCTCGATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGACTTATGAGGAGTTCCTGGAGGTGGACTTCTCGTTCGAAATCGACGGCTTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGTGGCGCGGCCGCGGTGTTCCGCACCATTCCCAGCAAGGTGCTGACGCTGGA
GCAGCTCAATGCGCCGCGCATCTTTGCCGATTTGGCGCTCAAGCCGCGTGGCCTGATTCTGGTGACCGGGCCGACCGGCT
CTGGCAAGTCCACCACGCTGGCGGCGATGATCAACCACCTCAACGAGAGCGAATACGGCCACATCCTGACGGTGGAGGAT
CCAATCGAATTCGTGCACGAGAGCAAGAAGTGCCTGATCAACCAACGCGAAGTCGGGCCGATGACGCACTCGTTCACGGC
TGCGCTGCGCTCTGCGCTGCGCGAAGACCCGGACGCCATTCTGGTGGGCGAATTGCGCGACCTGGAGACCATCCGCCTGG
CCCTGACGGCGGCCGAGACCGGCCACTTGGTGTTCGGCACGCTGCACACCTCCAGCGCGGCCAAGACCATCGACCGCGTG
ATCGACGTTTTCCCCGCCGAGGAAAAGGAGATGGTGCGCTCCATGCTGTCTGAATCGCTGGAGGCCGTGATCTCGCAGAC
GCTGCTCAAGACCAAGGATGGCAGCTCGCGTGTGGCCGCGCATGAGATCATGCTGGGCACGCCCGCGATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTATTCAACCATCCAGACCAGCCAGAACCAGGGCATGCAGACCTTGGACCAGAAT
CTGGTGGAGCTGGTGCGGCGCAACGTCATCAGCGCACGCGACGCGCGCCTGAAGGCCAAGATGCCGGAAAACTTCCCCGT
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2ATU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas aeruginosa PAK

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

73.731

96.542

0.712
  pilT Legionella pneumophila strain ERS1305867

73.731

96.542

0.712
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Pseudomonas stutzeri DSM 10701

41.593

97.695

0.406

Multiple sequence alignment