Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAHN93_RS02250 Genome accession   NZ_CP154474
Coordinates   486582..487718 (+) Length   378 a.a.
NCBI ID   WP_095550180.1    Uniprot ID   -
Organism   Vandammella animalimorsus strain W29-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481582..492718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHN93_RS02230 (AAHN93_02230) - 482119..483117 (+) 999 WP_343293274.1 LD-carboxypeptidase -
  AAHN93_RS02235 (AAHN93_02235) - 483502..484458 (+) 957 WP_343293275.1 tripartite tricarboxylate transporter substrate-binding protein -
  AAHN93_RS02240 (AAHN93_02240) pilT 484752..485795 (+) 1044 WP_095542791.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHN93_RS02245 (AAHN93_02245) - 485862..486512 (+) 651 WP_124858275.1 cyclic nucleotide-binding domain-containing protein -
  AAHN93_RS02250 (AAHN93_02250) pilU 486582..487718 (+) 1137 WP_095550180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAHN93_RS02255 (AAHN93_02255) - 487789..488721 (+) 933 WP_095542794.1 NAD(P)-dependent oxidoreductase -
  AAHN93_RS02260 (AAHN93_02260) aroQ 488987..489466 (+) 480 WP_095542795.1 type II 3-dehydroquinate dehydratase -
  AAHN93_RS02265 (AAHN93_02265) - 489587..490228 (+) 642 WP_095542796.1 DUF6806 family protein -
  AAHN93_RS02270 (AAHN93_02270) murI 490360..491190 (-) 831 WP_343293276.1 glutamate racemase -
  AAHN93_RS02275 (AAHN93_02275) - 491461..491922 (+) 462 WP_343293969.1 low molecular weight protein-tyrosine-phosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41816.09 Da        Isoelectric Point: 7.0679

>NTDB_id=993554 AAHN93_RS02250 WP_095550180.1 486582..487718(+) (pilU) [Vandammella animalimorsus strain W29-2]
MERDQASKFINDLLRLMVSRNGSDLFITADFPPAIKVDGKVSKVSPQPLTPAHTLALARSIMSDKQAAEFERTKEANFAI
SPAGVGRFRVNAFVQQGHVGLVLRVIPLNVPTIDGLGLPQVLKDVAMSKRGLCILVGATGSGKSTSLAAMVDWRNEHSYG
HIVTIEDPVEFVHAHKNCVITQREIGLDTDNWEVALKNSLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQAMDRIINFFPEERRAQLLMDVSLNLRAIMSQRLIPRQDGKGRIAAIEVLLNTPRVADLIFKGEVAEIKEAMKLSRNQG
MQTFDQALFDLFEANVISYEDALRNADSLNDLRLQIKLHSTRARTKDLAAGTEHLSVV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=993554 AAHN93_RS02250 WP_095550180.1 486582..487718(+) (pilU) [Vandammella animalimorsus strain W29-2]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTGCGGCTGATGGTCAGCCGCAATGGCAGCGATTTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCTCCAAGGTCTCGCCGCAGCCGCTGACGCCGGCGCACA
CGCTGGCGCTGGCGCGCTCCATCATGAGCGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAGGCCAATTTCGCCATC
TCGCCCGCGGGCGTGGGGCGTTTTCGCGTCAACGCCTTCGTGCAGCAAGGCCACGTGGGCTTGGTGCTGCGCGTGATCCC
GCTGAATGTGCCCACCATCGATGGCCTGGGCCTGCCGCAGGTCCTCAAGGACGTGGCCATGAGCAAGCGCGGCCTGTGCA
TTTTGGTGGGCGCCACCGGCTCGGGCAAGTCCACCTCGCTGGCGGCCATGGTGGATTGGCGCAACGAGCATTCCTACGGC
CACATCGTCACCATCGAGGATCCGGTGGAATTCGTGCACGCGCACAAGAACTGCGTCATCACCCAGCGCGAGATCGGCCT
GGACACCGACAACTGGGAGGTGGCGCTGAAGAACTCGCTGCGTCAGGCGCCGGACGTGATCCTCATGGGTGAGATCCGCG
ACCGCGAGACCATGGAGCACGCCATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCTCC
AACCAGGCCATGGACCGCATCATCAACTTCTTCCCTGAGGAGCGCCGCGCCCAGCTGCTGATGGACGTGTCGCTGAACCT
GCGCGCCATCATGTCGCAGCGTCTGATCCCGCGCCAGGACGGCAAGGGGCGCATCGCTGCGATCGAGGTGCTGCTCAACA
CCCCGCGCGTGGCCGATTTGATCTTCAAGGGCGAGGTGGCTGAGATCAAGGAGGCCATGAAGCTCAGCCGCAACCAGGGC
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAGGCCAACGTCATCAGCTACGAAGATGCGCTGCGCAATGCCGA
CTCGCTCAATGATCTGCGCCTGCAGATCAAGCTGCACAGCACGCGCGCGCGCACCAAGGACTTGGCCGCTGGCACCGAAC
ACCTCTCCGTGGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.931

92.063

0.579
  pilU Acinetobacter baylyi ADP1

57.429

92.593

0.532
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Pseudomonas stutzeri DSM 10701

41.246

89.153

0.368
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365
  pilT Acinetobacter baylyi ADP1

41.441

88.095

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.265

87.831

0.362
  pilT Vibrio cholerae strain A1552

41.265

87.831

0.362

Multiple sequence alignment