Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMG91_RS16170 Genome accession   NZ_AP027049
Coordinates   3551265..3552302 (-) Length   345 a.a.
NCBI ID   WP_004398311.1    Uniprot ID   A0AAV2VQ50
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3546265..3557302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG91_RS16140 (TUMSATVNIG2_31490) - 3546499..3547200 (+) 702 WP_022549510.1 endonuclease -
  QMG91_RS16145 (TUMSATVNIG2_31500) rsmE 3547306..3548037 (+) 732 WP_281827655.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QMG91_RS16150 (TUMSATVNIG2_31510) gshB 3548052..3548999 (+) 948 WP_281827656.1 glutathione synthase -
  QMG91_RS16155 (TUMSATVNIG2_31520) - 3549026..3549589 (+) 564 WP_004409298.1 YqgE/AlgH family protein -
  QMG91_RS16160 (TUMSATVNIG2_31530) ruvX 3549642..3550067 (+) 426 WP_022549508.1 Holliday junction resolvase RuvX -
  QMG91_RS16165 (TUMSATVNIG2_31540) pilU 3550150..3551256 (-) 1107 WP_004398310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMG91_RS16170 (TUMSATVNIG2_31550) pilT 3551265..3552302 (-) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG91_RS16175 (TUMSATVNIG2_31560) - 3552326..3553036 (+) 711 WP_004398312.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMG91_RS16180 (TUMSATVNIG2_31570) proC 3553098..3553916 (+) 819 WP_281827657.1 pyrroline-5-carboxylate reductase -
  QMG91_RS16185 (TUMSATVNIG2_31580) - 3553946..3554503 (+) 558 WP_004398314.1 YggT family protein -
  QMG91_RS16190 (TUMSATVNIG2_31590) yggU 3554503..3554793 (+) 291 WP_022549505.1 DUF167 family protein YggU -
  QMG91_RS16195 (TUMSATVNIG2_31600) - 3554817..3555248 (+) 432 WP_281827658.1 DUF4426 domain-containing protein -
  QMG91_RS16200 (TUMSATVNIG2_31610) - 3555315..3555914 (+) 600 WP_022601627.1 XTP/dITP diphosphatase -
  QMG91_RS16205 (TUMSATVNIG2_31620) hemW 3555921..3557096 (+) 1176 WP_281827659.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38005.26 Da        Isoelectric Point: 6.2687

>NTDB_id=99342 QMG91_RS16170 WP_004398311.1 3551265..3552302(-) (pilT) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
MDIAELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFTHADVHRLVFDIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTEVPSLEQISAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHQSNKCLINQREVHRDTHSFNAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGTDKDMVRSMLSESLRAVISQKLLKRLGGGRAACHEIMLATPAIRNLIREDKVAQMYSVIQTGGAMGMQTTEQHA
KQLIAQGVVDADEVRTKLESDSMGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=99342 QMG91_RS16170 WP_004398311.1 3551265..3552302(-) (pilT) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCTGGAGTTCCTCCAAT
GGTCCGCGTAGACGGCGATGTACGTAAGTTGGGCGTGCCTGCTTTTACTCATGCTGATGTTCACCGGTTGGTTTTCGATA
TTATGAACGACGCGCAACGCGCTGAGTTCGAAGAAAAACTGGAAACGGACTTCTCTTTTGAACTGCCTAATGTTGGTCGC
TTCCGTGTGAACGCTTTTAATCAGTCACGCGGGTGCTCGGCGGTATTTCGAACCATTCCTACCGAAGTGCCAAGCCTGGA
GCAAATCAGTGCGCCTGATATTTTTACTCAAATTTCCAACTATGAGAAAGGGTTAGTATTGGTTACCGGTCCGACGGGTT
CGGGTAAATCCACCACACTTGCCGCCATGGTTGACCATATCAACCGCAATCACAACAAACACATTCTTACGATCGAAGAT
CCGATTGAGTTTGTACACCAAAGTAATAAATGCTTGATCAACCAACGAGAAGTTCATCGCGATACGCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGACCCGGATGTGATTCTGGTGGGTGAGCTTCGTGATAAGGAAACCATCAGCCTTG
CATTGACGGCTGCGGAAACGGGTCACTTGGTGTTTGGTACCTTGCACACCAGCAGTGCAGCCAAAACCGTCGACCGTATT
ATCGATGTGTTCCCTGGCACCGACAAAGACATGGTTCGCTCGATGCTCTCTGAATCGCTACGTGCGGTGATTTCTCAAAA
GCTGCTTAAGCGCTTGGGTGGTGGTCGTGCTGCCTGTCACGAAATCATGCTGGCAACACCAGCGATTCGAAACTTGATTC
GTGAAGACAAAGTCGCTCAGATGTATTCGGTTATCCAGACGGGTGGAGCTATGGGGATGCAGACCACAGAGCAGCATGCC
AAGCAGCTGATTGCTCAAGGGGTGGTCGATGCTGACGAGGTACGTACCAAACTTGAATCCGATTCTATGGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.797

100

0.858
  pilT Vibrio cholerae strain A1552

85.797

100

0.858
  pilT Acinetobacter baumannii D1279779

72.917

97.391

0.71
  pilT Acinetobacter baumannii strain A118

72.917

97.391

0.71
  pilT Acinetobacter nosocomialis M2

72.619

97.391

0.707
  pilT Acinetobacter baylyi ADP1

70

98.551

0.69
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Pseudomonas aeruginosa PAK

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

63.689

100

0.641
  pilT Neisseria gonorrhoeae MS11

63.401

100

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.812

0.49
  pilU Vibrio cholerae strain A1552

41.846

94.203

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment