Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMG46_RS09205 Genome accession   NZ_AP027042
Coordinates   2078289..2079326 (+) Length   345 a.a.
NCBI ID   WP_281852211.1    Uniprot ID   -
Organism   Dyella sp. GSA-30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2073289..2084326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG46_RS09170 (DYGSA30_18200) pdxH 2073557..2074144 (+) 588 WP_281852852.1 pyridoxamine 5'-phosphate oxidase -
  QMG46_RS09175 (DYGSA30_18210) - 2074155..2074598 (+) 444 WP_281852204.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  QMG46_RS09180 (DYGSA30_18220) - 2074595..2075107 (+) 513 WP_281852205.1 hypothetical protein -
  QMG46_RS09185 (DYGSA30_18230) - 2075165..2075968 (+) 804 WP_281852206.1 cobalamin-binding protein -
  QMG46_RS09190 (DYGSA30_18240) - 2075940..2076554 (-) 615 WP_281852207.1 hypothetical protein -
  QMG46_RS09195 (DYGSA30_18250) - 2076577..2077164 (-) 588 WP_281852208.1 YggT family protein -
  QMG46_RS09200 (DYGSA30_18260) proC 2077161..2077991 (-) 831 WP_281852209.1 pyrroline-5-carboxylate reductase -
  QMG46_RS09205 (DYGSA30_18270) pilT 2078289..2079326 (+) 1038 WP_281852211.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG46_RS09210 (DYGSA30_18280) pilU 2079349..2080515 (+) 1167 WP_281852212.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMG46_RS09215 - 2080527..2082920 (-) 2394 WP_281852213.1 fimbria/pilus outer membrane usher protein -
  QMG46_RS09220 (DYGSA30_18300) - 2082999..2083709 (-) 711 WP_281852214.1 DUF1120 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38488.16 Da        Isoelectric Point: 6.8887

>NTDB_id=99246 QMG46_RS09205 WP_281852211.1 2078289..2079326(+) (pilT) [Dyella sp. GSA-30]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFFECDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLAAPRIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHILTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDFILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQTLMKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQNL
LDLVKRGLVTRQAAGSYAKNKEQFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=99246 QMG46_RS09205 WP_281852211.1 2078289..2079326(+) (pilT) [Dyella sp. GSA-30]
ATGGATATCGCCGAACTACTTGCCTTCTCGGTGAAGAACAAGGCATCGGACTTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGTGTGGACGGCGACGTGCGCCGCATCAATATTCCGGCGCTGGAGCACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAATTCTTCGAGTGCGACTTCTCGTTCGAAATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCTTCCGAGGTGCTGACCCTGGA
AGACCTGGCTGCGCCGCGCATCTTCCGCGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGATCACATCAACAAGAACGAATACGGCCATATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAAGTGCATCGCGATACGCACGGCTTCAACGA
GGCATTGCGTTCGGCGCTGCGCGAAGATCCCGACTTCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGACTGG
CGCTGACCGCTGCCGAAACCGGCCACTTGGTGTTCGGCACGCTGCATACCAGTTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAACCGATGGTCCGCTCGATGCTGTCCGAGTCGCTACGCGCGGTTATCTCGCAGAC
GCTGATGAAGAAAGTGGGCGGCGGTCGTATCGCGGCTCACGAGATCATGGTCGGTATTCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTCGACCAGAACCTG
CTCGACCTGGTCAAGCGCGGTCTGGTCACGCGTCAGGCGGCCGGTAGCTACGCGAAGAACAAAGAGCAGTTTAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.833

97.391

0.69
  pilT Vibrio cholerae strain A1552

70.833

97.391

0.69
  pilT Neisseria gonorrhoeae MS11

64.348

100

0.643
  pilT Neisseria meningitidis 8013

64.348

100

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

100

0.481
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391
  pilU Acinetobacter baylyi ADP1

38.905

100

0.391

Multiple sequence alignment