Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA521A_RS14755 Genome accession   NZ_AP027041
Coordinates   3239316..3240353 (-) Length   345 a.a.
NCBI ID   WP_281779623.1    Uniprot ID   -
Organism   Lysobacter auxotrophicus strain 5-21a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3234316..3245353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA521A_RS14735 (LA521A_29070) - 3234630..3234797 (-) 168 WP_281779619.1 anti-anti-sigma factor -
  LA521A_RS14740 (LA521A_29080) - 3234871..3235326 (+) 456 WP_281779620.1 hypothetical protein -
  LA521A_RS14745 (LA521A_29090) - 3235351..3237636 (+) 2286 WP_281779621.1 ATP-binding protein -
  LA521A_RS14750 (LA521A_29100) pilU 3238011..3239186 (-) 1176 WP_281779622.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LA521A_RS14755 (LA521A_29110) pilT 3239316..3240353 (-) 1038 WP_281779623.1 type IV pilus twitching motility protein PilT Machinery gene
  LA521A_RS14760 (LA521A_29120) - 3240465..3241148 (+) 684 WP_281779624.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA521A_RS14765 (LA521A_29130) proC 3241201..3242037 (+) 837 WP_281779625.1 pyrroline-5-carboxylate reductase -
  LA521A_RS14770 (LA521A_29140) - 3242056..3242553 (+) 498 WP_281779626.1 DUF4426 domain-containing protein -
  LA521A_RS14775 (LA521A_29150) - 3243224..3243415 (+) 192 WP_281779627.1 hypothetical protein -
  LA521A_RS14780 (LA521A_29160) - 3243637..3244434 (-) 798 WP_281779628.1 cobalamin-binding protein -
  LA521A_RS14785 (LA521A_29170) aac(6') 3244431..3244892 (-) 462 WP_281779629.1 aminoglycoside 6'-N-acetyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38575.37 Da        Isoelectric Point: 6.7269

>NTDB_id=99237 LA521A_RS14755 WP_281779623.1 3239316..3240353(-) (pilT) [Lysobacter auxotrophicus strain 5-21a]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLDDLACPPIFRELIQQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQNYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=99237 LA521A_RS14755 WP_281779623.1 3239316..3240353(-) (pilT) [Lysobacter auxotrophicus strain 5-21a]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTCAAGAACAAAGCATCCGACCTGCATCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAAATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTCTTCCGTACGATTCCGTCGGAAGTGCTCACGCTGGA
CGATCTGGCCTGCCCGCCGATCTTCCGCGAGCTCATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCTCCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTTCGCGAAGACCCCGACTACATCCTCGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACTGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACGGTGGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGTC
GCTGTTGAAGAAGGTCGGCGGCGGTCGTACCGCGGCATGGGAAATCATGGTCGGCATCCCGGCCATCCGTAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGAACTACGGAATGATGACGCTCGACCAGCACCTG
CAGGACCTCGTCAAGCGCGGCCTCATCCTGCGTCCGCAGGCGAAGGAATACGCGAAGGACAAGCGCTTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.286

98.261

0.759
  pilT Legionella pneumophila strain Lp02

73.837

99.71

0.736
  pilT Legionella pneumophila strain ERS1305867

73.837

99.71

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Pseudomonas stutzeri DSM 10701

40.909

95.652

0.391
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38

Multiple sequence alignment