Detailed information NTG0099236

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LA521A_RS14750
• Genome accession: NZ_AP027041
• Coordinates: 3238011..3239186 (-)
• Length: 391 a.a.
• NCBI ID: WP_281779622.1
• Uniprot ID: -
• Organism: Lysobacter auxotrophicus strain 5-21a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3233011..3244186

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LA521A_RS14735 (LA521A_29070)	-	3234630..3234797 (-)	168	WP_281779619.1	anti-anti-sigma factor	-
LA521A_RS14740 (LA521A_29080)	-	3234871..3235326 (+)	456	WP_281779620.1	hypothetical protein	-
LA521A_RS14745 (LA521A_29090)	-	3235351..3237636 (+)	2286	WP_281779621.1	ATP-binding protein	-
LA521A_RS14750 (LA521A_29100)	pilU	3238011..3239186 (-)	1176	WP_281779622.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LA521A_RS14755 (LA521A_29110)	pilT	3239316..3240353 (-)	1038	WP_281779623.1	type IV pilus twitching motility protein PilT	Machinery gene
LA521A_RS14760 (LA521A_29120)	-	3240465..3241148 (+)	684	WP_281779624.1	YggS family pyridoxal phosphate-dependent enzyme	-
LA521A_RS14765 (LA521A_29130)	proC	3241201..3242037 (+)	837	WP_281779625.1	pyrroline-5-carboxylate reductase	-
LA521A_RS14770 (LA521A_29140)	-	3242056..3242553 (+)	498	WP_281779626.1	DUF4426 domain-containing protein	-
LA521A_RS14775 (LA521A_29150)	-	3243224..3243415 (+)	192	WP_281779627.1	hypothetical protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43174.70 Da
• Isoelectric Point: 7.0157

>NTDB_id=99236 LA521A_RS14750 WP_281779622.1 3238011..3239186(-) (pilU) [Lysobacter auxotrophicus strain 5-21a]
MNTSTNGTTPAASAATGAVDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNALTPQQSRDMVLNVMNPQQREE
FEKTHECNFAIGVAGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAA
MIGYRNMNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGH
LVLCTLHANNANQAMDRIINFFPEDRRQQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKL
KELMKESVQLGMKTFDQALFELYQAGDISYEDALRYADSQNEVRLRIKLAQGGDARTLAAGLDGVELAQVR

Nucleotide

• Length: 1176 bp

>NTDB_id=99236 LA521A_RS14750 WP_281779622.1 3238011..3239186(-) (pilU) [Lysobacter auxotrophicus strain 5-21a]
ATGAACACCAGCACCAACGGCACCACGCCCGCCGCCAGCGCCGCCACCGGCGCCGTCGACTTCACCTCGTTCCTGAAGCT
GATGGCGCACCAGAAGGCGTCCGACCTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCTCGC
CGATCACCCAGAACGCGCTGACGCCGCAGCAGTCCCGCGACATGGTGCTCAACGTGATGAACCCGCAGCAGCGCGAGGAG
TTCGAGAAGACGCACGAGTGCAACTTCGCCATCGGCGTTGCGGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCG
CAACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACCAAGATCCCGACGGTGGAGGAGCTGAACCTGCCGCCGGTGATCA
AGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGTGCCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCG
ATGATCGGCTATCGCAACATGAACTCGACCGGCCACATCATCACCATCGAGGACCCGATCGAATTCGTGCACAAGCACGA
GGGCTGCATCATCACGCAGCGCGAAGTGGGCATCGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGG
CGCCGGACGTGATCATGATCGGCGAGGTGCGCACGCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCAC
CTGGTGCTGTGCACGCTGCACGCGAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCG
CCAGCAGCTGCTGATGGATCTTTCGCTCAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATCCCGACGCCGGACGGCAAGG
GCCGTCGCGTCGCGATGGAAATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTC
AAGGAACTGATGAAGGAATCCGTGCAGCTGGGCATGAAGACCTTCGACCAGGCGCTGTTCGAGCTTTACCAGGCCGGCGA
CATCTCCTACGAGGACGCGCTGCGTTACGCCGACTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCGCGCAGGGCGGGG
ACGCGCGCACGCTGGCGGCCGGCCTCGACGGCGTGGAGCTGGCGCAGGTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (133-287)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.022	94.373	0.642
pilU	Acinetobacter baylyi ADP1	64.426	91.304	0.588
pilU	Vibrio cholerae strain A1552	53.933	91.049	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.751	95.396	0.389