Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAFF19_RS03435 Genome accession   NZ_CP152407
Coordinates   730398..731441 (-) Length   347 a.a.
NCBI ID   WP_008907101.1    Uniprot ID   H0C472
Organism   Acidovorax sp. FHTAMBA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725398..736441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF19_RS03405 (AAFF19_03405) - 725624..726025 (+) 402 WP_034695839.1 YraN family protein -
  AAFF19_RS03410 (AAFF19_03410) - 726125..726727 (+) 603 WP_008907096.1 SIS domain-containing protein -
  AAFF19_RS03415 (AAFF19_03415) - 726724..727392 (+) 669 WP_008907097.1 BON domain-containing protein -
  AAFF19_RS03420 (AAFF19_03420) - 727490..728404 (-) 915 WP_342721316.1 NAD(P)-dependent oxidoreductase -
  AAFF19_RS03425 (AAFF19_03425) pilU 728504..729640 (-) 1137 WP_182119639.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFF19_RS03430 (AAFF19_03430) - 729705..730340 (-) 636 WP_008907100.1 cyclic nucleotide-binding domain-containing protein -
  AAFF19_RS03435 (AAFF19_03435) pilT 730398..731441 (-) 1044 WP_008907101.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFF19_RS03440 (AAFF19_03440) - 731479..732198 (+) 720 WP_342721317.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFF19_RS03445 (AAFF19_03445) ltaE 732205..733275 (-) 1071 WP_008907103.1 low-specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38150.90 Da        Isoelectric Point: 6.7743

>NTDB_id=988915 AAFF19_RS03435 WP_008907101.1 730398..731441(-) (pilT) [Acidovorax sp. FHTAMBA]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHGMVYDIMSDSQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=988915 AAFF19_RS03435 WP_008907101.1 730398..731441(-) (pilT) [Acidovorax sp. FHTAMBA]
GTGGACATTACCCAACTGCTCGCGTTCAGCGTCAAGAACAAGGCCTCCGATCTTCACCTGTCCGCGGGCCTGCCGCCCAT
GATCCGGGTCCACGGCGATGTACGGCGCATCAATGTCGATGCGCTGGACCACAAGACGGTCCACGGCATGGTGTACGACA
TCATGAGCGACTCGCAGCGCAAGACGTATGAAGAGTTTCTCGAAGTCGACTTCTCGTTTGAAATCGAAGGCCTGGCCCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGGACGATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAATGCTCCCAAGATCTTCGGCGACCTGGCCCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTGAATTACCTCAATGAAACCGAATACGGCCACATCCTCACAGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCCAAAAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCGGC
GGCGCTCAAGTCGGCACTGCGTGAAGACCCGGACGCCATCCTGGTGGGTGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCTGCGGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACATCGAGTGCCGCCAAGACGATTGACCGGATC
ATCGACGTGTTCCCGGCCGAAGAAAAGGAAATGGTTCGCGCGATGCTGTCCGAATCGCTGCAGGCAGTCATCTCGCAGAC
GCTGTGCAAGACCAAGGATGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAACATCATCAGCCCGGCCGAAGCCCGCAGCAAGGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H0C472

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment