Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAFF19_RS03425 Genome accession   NZ_CP152407
Coordinates   728504..729640 (-) Length   378 a.a.
NCBI ID   WP_182119639.1    Uniprot ID   -
Organism   Acidovorax sp. FHTAMBA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723504..734640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFF19_RS03400 (AAFF19_03400) rsmI 724665..725624 (-) 960 WP_182119637.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AAFF19_RS03405 (AAFF19_03405) - 725624..726025 (+) 402 WP_034695839.1 YraN family protein -
  AAFF19_RS03410 (AAFF19_03410) - 726125..726727 (+) 603 WP_008907096.1 SIS domain-containing protein -
  AAFF19_RS03415 (AAFF19_03415) - 726724..727392 (+) 669 WP_008907097.1 BON domain-containing protein -
  AAFF19_RS03420 (AAFF19_03420) - 727490..728404 (-) 915 WP_342721316.1 NAD(P)-dependent oxidoreductase -
  AAFF19_RS03425 (AAFF19_03425) pilU 728504..729640 (-) 1137 WP_182119639.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAFF19_RS03430 (AAFF19_03430) - 729705..730340 (-) 636 WP_008907100.1 cyclic nucleotide-binding domain-containing protein -
  AAFF19_RS03435 (AAFF19_03435) pilT 730398..731441 (-) 1044 WP_008907101.1 type IV pilus twitching motility protein PilT Machinery gene
  AAFF19_RS03440 (AAFF19_03440) - 731479..732198 (+) 720 WP_342721317.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAFF19_RS03445 (AAFF19_03445) ltaE 732205..733275 (-) 1071 WP_008907103.1 low-specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41878.30 Da        Isoelectric Point: 6.9078

>NTDB_id=988914 AAFF19_RS03425 WP_182119639.1 728504..729640(-) (pilU) [Acidovorax sp. FHTAMBA]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPTHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVSFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEIMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDLYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=988914 AAFF19_RS03425 WP_182119639.1 728504..729640(-) (pilU) [Acidovorax sp. FHTAMBA]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTGATGGTGAGCCGCAATGGCAGCGACCTGTTCAT
CACCGCAGAGTTTCCGCCCGCGATCAAGGTCGATGGCAAAGTGACCAAGGTCTCGCCCCAGCCGCTCACGCCAACGCACA
CGCTCACCCTGGCACGCGCCATCATGAGCGACAAGCAGGTGGCCGATTTTGAGCGCACCAAGGAATGCAACTTCGCCATT
TCACCCGCCGGCATCGGGCGGTTTCGCGTCAACGCTTTCATCCAGCAGGGCAAGGTGGGCATGGTGCTGCGGACGATTCC
GCTCACGCTGCCCACCATCGATGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCTACAGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGTCT
GGACACCGACAGCTGGGAAGCGGCCCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTCATGGGCGAAATCCGCG
ACCGTGAAACCATGGAGCATGCGGTGTCGTTCGCCGAAACCGGACACCTGTGCCTTGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTGATCCCCAAGCAGGATGGCAAGGGCCGCGCGGCCGCGGTGGAAATCATGCTCAATA
CGCCGCTGATTTCCGATCTGATCTTCAAGGGCGAAGTCTCCGAGATCAAGGAGATCATGAAAAAAAGCCGCAACCTGGGC
ATGCAGACGTTTGACCAGGCGCTGTTCGACCTGTACGAAGCCAACGTGATCAGCTACGAAGACGCGTTGCGCAACGCCGA
CTCGCTCAATGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAGTCTCCCGATCTGGCGTCCGGCACGGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

52.342

96.032

0.503
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment