Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGD16_RS12870 Genome accession   NZ_CP152390
Coordinates   2787905..2788945 (-) Length   346 a.a.
NCBI ID   WP_013465851.1    Uniprot ID   A0AA37S294
Organism   Pseudoalteromonas sp. M58     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2782905..2793945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD16_RS12840 - 2783247..2783720 (+) 474 WP_013465845.1 hypothetical protein -
  AAGD16_RS12845 rsmE 2783791..2784525 (+) 735 WP_273001729.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAGD16_RS12850 gshB 2784592..2785542 (+) 951 WP_013465847.1 glutathione synthase -
  AAGD16_RS12855 - 2785678..2786235 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  AAGD16_RS12860 ruvX 2786253..2786702 (+) 450 WP_377112214.1 Holliday junction resolvase RuvX -
  AAGD16_RS12865 pilU 2786759..2787895 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGD16_RS12870 pilT 2787905..2788945 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGD16_RS12875 - 2788948..2789661 (+) 714 WP_024600821.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGD16_RS12880 proC 2789679..2790500 (+) 822 WP_377112219.1 pyrroline-5-carboxylate reductase -
  AAGD16_RS12885 - 2790527..2791060 (+) 534 WP_024600823.1 YggT family protein -
  AAGD16_RS12890 - 2791119..2791562 (+) 444 WP_024600824.1 DUF4426 domain-containing protein -
  AAGD16_RS12895 - 2791565..2792164 (+) 600 WP_024600825.1 XTP/dITP diphosphatase -
  AAGD16_RS12900 hemW 2792161..2793297 (+) 1137 WP_024600826.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38244.49 Da        Isoelectric Point: 6.2312

>NTDB_id=988875 AAGD16_RS12870 WP_013465851.1 2787905..2788945(-) (pilT) [Pseudoalteromonas sp. M58]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALGDKEVSSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPEIFKQISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHVLTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMIRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLATPAIRNLIREDKIAQMYSSIQTGASQGMQTMDQCL
TSLVNRGIITHSAAYTKAQDKTQFSA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=988875 AAGD16_RS12870 WP_013465851.1 2787905..2788945(-) (pilT) [Pseudoalteromonas sp. M58]
ATGGATATCACCGAATTATTGGCATTTAGTGTGCAACATAAAGCATCAGATTTGCACTTATCATCAGGGGTATCACCTAT
GATACGTGTTGATGGCGATGTGCGACGTATTAATATACCAGCACTTGGAGATAAAGAAGTCAGCAGTCTGGTTTATGACA
TTATGAACGATAACCAGCGTAAGGACTATGAACAAAATCTCGAAGTGGATTTTTCTTTTGAAGTCCCTAATTTAGCACGT
TTTCGTGTAAATGCCTTTAACTCTAATCGTGGCCCTGCTGCTGTGTTTCGTACTATCCCGAGTGACGTACTCACTCTTGA
AGATTTAGGCGCACCTGAAATATTTAAACAAATTTCAGATAACCCACGAGGCCTAGTGTTAGTCACGGGCCCTACAGGTT
CAGGTAAGTCGACCACGCTTGCGGCGATGGTCGATTACATTAATCAGACTAAGCATCATCATGTGCTGACCATCGAAGAT
CCTATCGAATTTGTTCACGATAATAAACTCAGCTTAATTAATCAGCGGGAAGTTCATCGTGATACACATAGCTTTTCTAA
CGCACTACGCAGCGCACTCCGTGAAGACCCAGATGTTATTTTAGTGGGTGAGTTACGAGATTTAGAAACTATTCGCCTTG
CTATGACCGCTGCGGAAACCGGTCACTTGGTGTTTGGTACTTTGCATACGACCTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGCGAAGAAAAAGATATGATCCGCTCTATGCTCTCTGAGTCATTACGTGCAGTTATTTCGCAAAC
ACTGATAAAAAAAGTAGGGGGAGGGCGAGTGGCTGCACATGAAATTATGTTGGCAACGCCTGCTATTCGTAACTTAATCC
GTGAAGATAAAATTGCACAAATGTACTCATCAATTCAAACCGGTGCGTCGCAAGGCATGCAAACCATGGATCAATGTTTA
ACAAGCTTAGTTAACCGTGGCATTATCACTCACTCAGCGGCGTATACAAAAGCGCAAGATAAGACGCAGTTTAGCGCTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter nosocomialis M2

75.872

99.422

0.754
  pilT Acinetobacter baumannii D1279779

75.872

99.422

0.754
  pilT Acinetobacter baumannii strain A118

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Neisseria gonorrhoeae MS11

69.942

100

0.699
  pilT Neisseria meningitidis 8013

69.942

100

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.252

95.087

0.697
  pilT Vibrio cholerae strain A1552

73.252

95.087

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

100

0.523
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.643

97.977

0.379

Multiple sequence alignment