Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGD16_RS12865 Genome accession   NZ_CP152390
Coordinates   2786759..2787895 (-) Length   378 a.a.
NCBI ID   WP_013465850.1    Uniprot ID   A0AA37S1K5
Organism   Pseudoalteromonas sp. M58     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2781759..2792895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD16_RS12835 - 2782276..2783169 (+) 894 WP_013465844.1 hypothetical protein -
  AAGD16_RS12840 - 2783247..2783720 (+) 474 WP_013465845.1 hypothetical protein -
  AAGD16_RS12845 rsmE 2783791..2784525 (+) 735 WP_273001729.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAGD16_RS12850 gshB 2784592..2785542 (+) 951 WP_013465847.1 glutathione synthase -
  AAGD16_RS12855 - 2785678..2786235 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  AAGD16_RS12860 ruvX 2786253..2786702 (+) 450 WP_377112214.1 Holliday junction resolvase RuvX -
  AAGD16_RS12865 pilU 2786759..2787895 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGD16_RS12870 pilT 2787905..2788945 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGD16_RS12875 - 2788948..2789661 (+) 714 WP_024600821.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGD16_RS12880 proC 2789679..2790500 (+) 822 WP_377112219.1 pyrroline-5-carboxylate reductase -
  AAGD16_RS12885 - 2790527..2791060 (+) 534 WP_024600823.1 YggT family protein -
  AAGD16_RS12890 - 2791119..2791562 (+) 444 WP_024600824.1 DUF4426 domain-containing protein -
  AAGD16_RS12895 - 2791565..2792164 (+) 600 WP_024600825.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41994.04 Da        Isoelectric Point: 6.2895

>NTDB_id=988874 AAGD16_RS12865 WP_013465850.1 2786759..2787895(-) (pilU) [Pseudoalteromonas sp. M58]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTKIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRVAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=988874 AAGD16_RS12865 WP_013465850.1 2786759..2787895(-) (pilU) [Pseudoalteromonas sp. M58]
ATGACTTTATCTTTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCGAGCCAACTTCC
TATTAGCGCTAAAATTAACGGCGAGCTAACTCCTCTTAATGACGATAAATTAACAGATGAGCAAGCGCTAGAGTTAGTCG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAACACTAAAGAATGTAACTTTGCGATTGCAACTAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGCATGGTTATTCGTCGAATTGTCACTAAAATACCCGATGT
TAAAGAGCTTGGATTACCCTCTGCATTGACCGATGTGATTATGTCAAAGCGCGGACTCGTGTTATTTGTAGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCACTTATTGGTTATAGAAATCGCAATCAACGTGGGCATATTTTAACCATAGAA
GATCCGATTGAATTTGTGCATGAACATCAAAAAAGTATTATTACCCAACGTGAAGTGGGTTTAGATACTGAAAGCTTTGA
ATCGGCACTTAAAAGCTCTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGTTCGCAAGAAACCATGGAAT
ATGCGTTGAGCTTTGCAGAAACGGGTCATTTATGTGTTGCCACCTTGCATGCTAACAATGCTAATCAAGCTATTGACCGC
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTTAAATATGACTTAGCATTAAATTTACGCGCTATCATTGCTCA
GCAACTTATTCCTACCTCTGATGGGGAAGGGCGTGTTGCTGCCATTGAGGTATTACTTAATACGCCAATGGTCAGTGAGC
TAATTAAAAACGGTAATATTGGTGGCATTAAAGAAACCATGGCTAAATCAACAGAGATGGGCATGCAAACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGTATTAATTATGCCGATGCGCTGCATCATGCGGATTCGCCAAATGACTTACG
CTTGATGATTAAACTACGTAATAACGAGCAACAAGGCGCAGGCTTCTTACAAGGTGTGACCATTGATGGCCTTGATGACA
AAGGCAATATGACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.56

99.735

0.574
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Vibrio cholerae strain A1552

56.131

97.09

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment