Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGF34_RS02925 Genome accession   NZ_CP152376
Coordinates   631743..632786 (+) Length   347 a.a.
NCBI ID   WP_342619137.1    Uniprot ID   -
Organism   Rhodoferax sp. GW822-FHT02A01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 626743..637786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGF34_RS02910 (AAGF34_02910) - 627199..630225 (-) 3027 WP_342619134.1 L-glutamate gamma-semialdehyde dehydrogenase -
  AAGF34_RS02915 (AAGF34_02915) - 630505..630990 (+) 486 WP_342619135.1 Lrp/AsnC ligand binding domain-containing protein -
  AAGF34_RS02920 (AAGF34_02920) - 631003..631698 (-) 696 WP_342619136.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGF34_RS02925 (AAGF34_02925) pilT 631743..632786 (+) 1044 WP_342619137.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGF34_RS02930 (AAGF34_02930) pilU 632826..633962 (+) 1137 WP_342619138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGF34_RS02935 (AAGF34_02935) - 633966..634874 (+) 909 WP_342619139.1 NAD(P)-dependent oxidoreductase -
  AAGF34_RS02940 (AAGF34_02940) - 634939..635571 (-) 633 WP_342619140.1 BON domain-containing protein -
  AAGF34_RS02945 (AAGF34_02945) - 635568..636167 (-) 600 WP_342619141.1 phosphoheptose isomerase -
  AAGF34_RS02950 (AAGF34_02950) - 636266..636655 (-) 390 WP_342619142.1 YraN family protein -
  AAGF34_RS02955 (AAGF34_02955) rsmI 636657..637571 (+) 915 WP_342619143.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38269.00 Da        Isoelectric Point: 6.9353

>NTDB_id=988739 AAGF34_RS02925 WP_342619137.1 631743..632786(+) (pilT) [Rhodoferax sp. GW822-FHT02A01]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEPLDHKQVHSMVYDIMNDTQRKHYEEFLEIDYSFEIDGLAR
FRVNAFNHQRGAGAVFRTIPSKILSLEQLNAPKIFGDLALKPRGMVLVTGPTGSGKSTTLAAMVNYLNETEFGHILTVED
PIEFVHESKKCLINQREVGPHTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LADLVKRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=988739 AAGF34_RS02925 WP_342619137.1 631743..632786(+) (pilT) [Rhodoferax sp. GW822-FHT02A01]
GTGGACATCACCCAATTGCTGGCGTTCAGCGTCAAGAACAAAGCGTCGGACCTTCACCTCTCAGCTGGCTTGCCACCCAT
GATCCGTGTGCACGGTGACGTGCGCCGCATCAACGTGGAGCCACTGGACCACAAGCAGGTGCACAGCATGGTGTACGACA
TCATGAATGACACCCAGCGCAAGCATTACGAAGAGTTTCTGGAGATCGACTATTCGTTCGAGATCGACGGACTGGCACGC
TTCCGGGTCAATGCGTTCAACCACCAGCGCGGCGCCGGCGCCGTGTTCCGTACCATTCCCAGCAAGATCCTCTCGCTGGA
ACAATTGAATGCGCCCAAGATTTTTGGCGACCTGGCGCTCAAACCACGCGGCATGGTGCTGGTGACCGGCCCCACCGGTT
CGGGCAAATCCACCACCCTGGCTGCCATGGTCAACTACCTCAACGAAACCGAGTTCGGCCACATTCTGACGGTGGAAGAC
CCCATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTTGGTCCGCACACCCTGTCGTTTGCCGC
TGCCCTGAAGTCCGCGCTGCGGGAAGACCCGGACGCCATTCTGGTGGGCGAAATGCGTGATCTGGAAACCATTCGCCTGG
CCATGACCGCCGCGGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACCTCCAGTGCCGCCAAGACCATTGACCGGATC
ATTGACGTGTTCCCGGCCGACGAAAAAGAGATGGTGCGTGCCATGCTGTCCGAGTCACTGCAGGCCGTGATTTCGCAAAC
GCTGTGCAAGACCAAGGACGGCCAGGGTCGCGTGGCGGCACATGAAATCATGCTGGGCACCAGCGCCATCCGCAATCTGA
TCCGTGAAGCCAAGGTGGCGCAGATGTACTCCAGCATCCAGACCGGTAACAGCGTCGGCATGCAGACGCTGGACCAGAAT
CTGGCAGACTTGGTCAAGCGCAACATCATCAGCCCGGCAGAGGCACGCAGCAAGGCCAAGATCCCAGAAAACTTCCCAGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment