Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGF34_RS02930 Genome accession   NZ_CP152376
Coordinates   632826..633962 (+) Length   378 a.a.
NCBI ID   WP_342619138.1    Uniprot ID   -
Organism   Rhodoferax sp. GW822-FHT02A01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 627826..638962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGF34_RS02915 (AAGF34_02915) - 630505..630990 (+) 486 WP_342619135.1 Lrp/AsnC ligand binding domain-containing protein -
  AAGF34_RS02920 (AAGF34_02920) - 631003..631698 (-) 696 WP_342619136.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGF34_RS02925 (AAGF34_02925) pilT 631743..632786 (+) 1044 WP_342619137.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGF34_RS02930 (AAGF34_02930) pilU 632826..633962 (+) 1137 WP_342619138.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGF34_RS02935 (AAGF34_02935) - 633966..634874 (+) 909 WP_342619139.1 NAD(P)-dependent oxidoreductase -
  AAGF34_RS02940 (AAGF34_02940) - 634939..635571 (-) 633 WP_342619140.1 BON domain-containing protein -
  AAGF34_RS02945 (AAGF34_02945) - 635568..636167 (-) 600 WP_342619141.1 phosphoheptose isomerase -
  AAGF34_RS02950 (AAGF34_02950) - 636266..636655 (-) 390 WP_342619142.1 YraN family protein -
  AAGF34_RS02955 (AAGF34_02955) rsmI 636657..637571 (+) 915 WP_342619143.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AAGF34_RS02960 (AAGF34_02960) - 637587..637907 (-) 321 WP_342619144.1 ATPase with chaperone activity -
  AAGF34_RS02965 (AAGF34_02965) - 637931..638239 (-) 309 WP_342619145.1 high-potential iron-sulfur protein -
  AAGF34_RS02970 (AAGF34_02970) - 638355..638525 (-) 171 WP_342619146.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41946.28 Da        Isoelectric Point: 6.8440

>NTDB_id=988740 AAGF34_RS02930 WP_342619138.1 632826..633962(+) (pilU) [Rhodoferax sp. GW822-FHT02A01]
MERDQATKFINDLLKLMISRNGSDLFITADFPPAIKVDGKVTKVSPQPLNSAHTLALARSIMNDKQVAEFERTKEGNFAI
SPPGIGRFRVNVFIQQGKVGLVLRVIPANLPTIDGLGVPQVLKEIVQSKRGLCIMVGSTGSGKSTTLAAMVDWRNENSYG
HIITIEDPVEFVHAHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKALVSQRLIPKQDGKGRMAAVEVMINSPLISDLIFKGEVSEVKEIMKKSRNLG
MQTFDQALFDLYENNQITYEDALRNADSLNDLRLQIKLNSVRGKSQDLSAGTENLAIM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=988740 AAGF34_RS02930 WP_342619138.1 632826..633962(+) (pilU) [Rhodoferax sp. GW822-FHT02A01]
ATGGAACGCGACCAGGCAACCAAGTTCATCAATGACCTGCTCAAGCTGATGATCAGTCGCAATGGCAGCGACCTGTTCAT
CACCGCGGACTTCCCGCCAGCCATCAAAGTGGATGGCAAGGTCACCAAGGTGTCGCCCCAGCCACTCAATTCCGCACACA
CGCTGGCGTTGGCACGTTCCATCATGAACGACAAGCAGGTCGCGGAGTTTGAGCGCACCAAGGAAGGCAACTTTGCCATC
TCGCCACCCGGCATTGGCCGGTTCCGGGTCAATGTGTTCATCCAGCAAGGCAAAGTCGGCCTGGTGCTGCGGGTCATCCC
GGCCAATCTGCCCACCATTGACGGCCTGGGTGTTCCCCAGGTGCTCAAGGAAATCGTACAGTCCAAGCGCGGGCTGTGCA
TCATGGTGGGCTCCACCGGCTCGGGCAAGTCGACCACCCTGGCGGCCATGGTGGACTGGCGCAATGAAAATTCCTATGGG
CACATCATCACCATCGAAGACCCGGTGGAATTTGTGCACGCCCACAAGAATTGCGTAGTCACACAGCGCGAAGTGGGGTT
GGATACCGACAGCTGGGAGGCGGCTCTGAAGAACACCTTGCGCCAGGCACCGGACGTGATTCTCATGGGCGAAATCCGCG
ACCGGGAAACCATGGAGCACGCGGTTGCCTTTGCGGAAACCGGTCACCTGTGCTTGGCCACCTTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGTTGCTCATGGACCTGTCACTCAACCT
CAAGGCGCTGGTATCGCAGCGCCTCATCCCCAAACAGGATGGCAAGGGCCGCATGGCCGCGGTGGAGGTGATGATCAACT
CCCCTCTGATATCCGACTTGATCTTCAAGGGCGAAGTCTCGGAGGTGAAGGAGATCATGAAGAAGAGCCGCAACTTGGGT
ATGCAAACGTTTGACCAGGCGCTGTTCGATCTCTACGAGAACAATCAGATCACCTATGAAGATGCCTTGCGCAATGCCGA
CTCGCTCAACGACTTGCGCCTGCAGATTAAACTCAACAGCGTGCGCGGCAAGTCGCAAGACCTGAGTGCCGGCACCGAAA
ACCTCGCCATCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.368

92.063

0.593
  pilU Acinetobacter baylyi ADP1

57.939

94.974

0.55
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Acinetobacter baylyi ADP1

43.789

85.185

0.373

Multiple sequence alignment