Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAG689_RS19535 Genome accession   NZ_CP152342
Coordinates   3991767..3992810 (+) Length   347 a.a.
NCBI ID   WP_020228894.1    Uniprot ID   A0A0A1VI70
Organism   Acidovorax sp. SDU_ACID1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3986767..3997810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG689_RS19515 - 3987533..3988918 (+) 1386 WP_406623400.1 efflux transporter outer membrane subunit -
  AAG689_RS19520 - 3988922..3989836 (-) 915 WP_406623401.1 LysR family transcriptional regulator -
  AAG689_RS19525 ltaE 3989922..3990988 (+) 1067 Protein_3847 low-specificity L-threonine aldolase -
  AAG689_RS19530 - 3991015..3991725 (-) 711 Protein_3848 YggS family pyridoxal phosphate-dependent enzyme -
  AAG689_RS19535 pilT 3991767..3992810 (+) 1044 WP_020228894.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG689_RS19540 - 3992890..3993519 (+) 630 WP_406623402.1 cyclic nucleotide-binding domain-containing protein -
  AAG689_RS19545 pilU 3993563..3994699 (+) 1137 WP_020228892.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG689_RS19550 - 3994776..3995705 (+) 930 WP_406623403.1 NAD(P)-dependent oxidoreductase -
  AAG689_RS19555 - 3995770..3996426 (-) 657 WP_406623404.1 BON domain-containing protein -
  AAG689_RS19560 - 3996423..3997006 (-) 584 Protein_3854 SIS domain-containing protein -
  AAG689_RS19565 - 3997068..3997474 (-) 407 Protein_3855 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38090.89 Da        Isoelectric Point: 6.8355

>NTDB_id=988495 AAG689_RS19535 WP_020228894.1 3991767..3992810(+) (pilT) [Acidovorax sp. SDU_ACID1]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKAYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLSLKPRGLVLVTGPTGSGKSTTLAAMINHLNETEYGHILTIED
PIEFVHESKKCLVNQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSAGMQTLDQN
LTELVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=988495 AAG689_RS19535 WP_020228894.1 3991767..3992810(+) (pilT) [Acidovorax sp. SDU_ACID1]
GTGGACATCACCCAACTGCTCGCCTTCAGCGTGAAAAACAAGGCGTCGGACCTGCACCTTTCCGCAGGCCTGCCGCCGAT
GATCCGGGTCCACGGGGATGTACGCCGCATCAATGTGGATGCGCTGGACCACAAGACCGTCCACGCCATGGTGTACGACA
TCATGAGCGATGCGCAGCGCAAGGCCTACGAGGAGTTCCTGGAAGTCGATTTCTCGTTCGAGATCGACGGCCTGGCGCGC
TTTCGCGTCAACGCGTTCAACCAGAACCGCGGTGCGGCCGCCGTGTTCCGGACCATCCCGAGCAAGATCCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTCGCCGACCTGTCCCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACGGGCT
CGGGCAAGTCCACTACGCTGGCAGCGATGATCAACCACCTCAACGAGACGGAGTACGGCCACATTCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGGTGAACCAGCGCGAGGTGGGGCCGATGACGCTGTCGTTCGCCGC
GGCGCTCAAGTCCGCGCTGCGCGAAGACCCGGATGCCATCCTCGTCGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CCATGACGGCGGCCGAGACAGGCCACCTGGTGTTCGGCACGCTGCACACGTCGAGCGCCGCCAAGACCATCGACCGGATC
ATCGATGTGTTCCCCGCCGAGGAAAAGGAAATGGTGCGGGCGATGCTGTCGGAGTCGCTCCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGTTCCGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCACAGATGTATTCCACCATCCAGACGGGCAACAGCGCGGGCATGCAGACGCTGGACCAGAAT
CTCACCGAATTGGTGCGCCGCAACATCATCAGTCCCGCCGAAGCGCGCAGCAAGGCTAAGATTCCGGAGAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A1VI70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.582

96.542

0.421
  pilU Acinetobacter baylyi ADP1

40.462

99.712

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment