Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAG689_RS19545 Genome accession   NZ_CP152342
Coordinates   3993563..3994699 (+) Length   378 a.a.
NCBI ID   WP_020228892.1    Uniprot ID   A0A0A1VGX8
Organism   Acidovorax sp. SDU_ACID1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3988563..3999699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG689_RS19520 - 3988922..3989836 (-) 915 WP_406623401.1 LysR family transcriptional regulator -
  AAG689_RS19525 ltaE 3989922..3990988 (+) 1067 Protein_3847 low-specificity L-threonine aldolase -
  AAG689_RS19530 - 3991015..3991725 (-) 711 Protein_3848 YggS family pyridoxal phosphate-dependent enzyme -
  AAG689_RS19535 pilT 3991767..3992810 (+) 1044 WP_020228894.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG689_RS19540 - 3992890..3993519 (+) 630 WP_406623402.1 cyclic nucleotide-binding domain-containing protein -
  AAG689_RS19545 pilU 3993563..3994699 (+) 1137 WP_020228892.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG689_RS19550 - 3994776..3995705 (+) 930 WP_406623403.1 NAD(P)-dependent oxidoreductase -
  AAG689_RS19555 - 3995770..3996426 (-) 657 WP_406623404.1 BON domain-containing protein -
  AAG689_RS19560 - 3996423..3997006 (-) 584 Protein_3854 SIS domain-containing protein -
  AAG689_RS19565 - 3997068..3997474 (-) 407 Protein_3855 YraN family protein -
  AAG689_RS19570 rsmI 3997474..3998400 (+) 927 WP_406623405.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AAG689_RS19580 rplQ 3998605..3998997 (-) 393 WP_406623406.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41771.24 Da        Isoelectric Point: 6.9661

>NTDB_id=988496 AAG689_RS19545 WP_020228892.1 3993563..3994699(+) (pilU) [Acidovorax sp. SDU_ACID1]
MERDQASKFINDLLKLMVSRGGSDLFITADFPPAIKVDGKVTKVSPQPLTPTHTLTLARSIMSDKQVADFERTKECNFAI
APAGVGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGLPQVLKEITMTKRGLCILVGATGSGKSTSLAAMVDWRNEHSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLLPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVVTYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=988496 AAG689_RS19545 WP_020228892.1 3993563..3994699(+) (pilU) [Acidovorax sp. SDU_ACID1]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTGATGGTCAGCCGGGGAGGGAGCGACCTGTTCAT
CACGGCGGACTTCCCTCCGGCCATCAAGGTGGACGGCAAGGTCACCAAGGTGTCGCCACAGCCGCTCACCCCCACGCACA
CCCTGACGCTGGCACGCTCGATCATGAGCGACAAGCAGGTGGCGGATTTCGAGCGCACCAAGGAATGCAATTTCGCCATC
GCGCCCGCAGGCGTCGGGCGCTTCCGCGTCAACGCCTTCATCCAGCAAGGCCGCGTGGGCATGGTGCTGAGGACCATTCC
GCTCACCCTGCCGACCATCGATGGCCTGGGCTTGCCGCAGGTGCTCAAGGAAATCACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAGTCGACCTCCCTGGCCGCCATGGTGGACTGGCGCAACGAGCACTCGTTCGGC
CACATCATCACGGTGGAGGACCCCATCGAGTTCGTGCATCCGCACAAGAACTGCGTCGTCACGCAGCGCGAGGTGGGCCT
GGATACCGACAGCTGGGAAGCCGCGTTGAAGAACACACTGCGCCAGGCGCCCGACGTTATCCTGATGGGCGAGATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTTCCCGAGGAACGCCGCGCGCAACTGCTCATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGCCTCCTTCCCAAGCAGGATGGCAAGGGCCGGGCCGCAGCGGTCGAGGTGATGCTCAACA
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACTTTCGACCAGGCACTGTTCGATCTGTTCGAGGCGAATGTGGTCACCTACGAGGATGCCTTGCGCAACGCGGA
CTCGGTGAACGATCTGCGCCTGCAGATCAAGCTGAGCAGCCAACGCGCCAAGACCACGGACCTCGCCTCCGGCACCGAAC
ACTTCGCCATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A1VGX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.756

92.328

0.598
  pilU Acinetobacter baylyi ADP1

58.659

94.709

0.556
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Acinetobacter nosocomialis M2

45.045

88.095

0.397
  pilT Acinetobacter baumannii D1279779

45.045

88.095

0.397
  pilT Acinetobacter baumannii strain A118

45.045

88.095

0.397
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Vibrio cholerae strain A1552

43.373

87.831

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.373

87.831

0.381

Multiple sequence alignment