Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAG562_RS04955 Genome accession   NZ_CP152340
Coordinates   1063511..1064545 (+) Length   344 a.a.
NCBI ID   WP_003282742.1    Uniprot ID   A0A2N8RHN6
Organism   Stutzerimonas stutzeri strain NCIMB_885     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1058511..1069545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG562_RS04935 (AAG562_04935) - 1059081..1061048 (-) 1968 WP_036991432.1 dynamin-like GTPase family protein -
  AAG562_RS04940 (AAG562_04940) - 1061320..1061910 (-) 591 WP_003282747.1 YggT family protein -
  AAG562_RS04945 (AAG562_04945) proC 1061920..1062744 (-) 825 WP_003282745.1 pyrroline-5-carboxylate reductase -
  AAG562_RS04950 (AAG562_04950) - 1062755..1063444 (-) 690 WP_003282744.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG562_RS04955 (AAG562_04955) pilT 1063511..1064545 (+) 1035 WP_003282742.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG562_RS04960 (AAG562_04960) pilU 1064631..1065776 (+) 1146 WP_003282740.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG562_RS04965 (AAG562_04965) - 1065773..1066609 (-) 837 WP_003282739.1 TIGR03915 family putative DNA repair protein -
  AAG562_RS04970 (AAG562_04970) - 1066609..1067820 (-) 1212 WP_003282738.1 putative DNA modification/repair radical SAM protein -
  AAG562_RS04975 (AAG562_04975) - 1067954..1068349 (+) 396 WP_003282737.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37916.65 Da        Isoelectric Point: 6.8360

>NTDB_id=988434 AAG562_RS04955 WP_003282742.1 1063511..1064545(+) (pilT) [Stutzerimonas stutzeri strain NCIMB_885]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=988434 AAG562_RS04955 WP_003282742.1 1063511..1064545(+) (pilT) [Stutzerimonas stutzeri strain NCIMB_885]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGAATCAACCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTTCTCGAGACTGACTTCTCGTTCGAAGTACCTGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAATCGGGGCGCCGGTGCGGTGTTCCGAACCATTCCCTCGAAAGTGCTGACCATGGA
AGATCTCGGCATGGGCGAGGTGTTTCGCAAGATCACCGACGTACCTCGGGGCTTGGTGCTGGTTACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCAATGCTCGATTATCTGAACAACACAAAGTACCACCACATTCTGACCATCGAAGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTCCATCGTGACACCCTGGGCTTCTCCGA
GGCGCTGCGTTCGGCACTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGTCACCTGGTATTTGGCACCCTGCACACCACATCGGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAGAAGTCCATGGTTCGCTCGATGCTCTCCGAGTCGTTGCAGGCGGTAGTCTCCCAAAC
GCTGCTGAAAAAGGTAGGCGGCGGCCGCGTGGCAGCCCACGAGATCATGATCGGTACGCCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATTCAGACCGGTGGTTCGCTGGGCATGCAGACGCTCGATTCGTGCCTC
AAGGGTCTGCTCGCCAAGGGGCTGATCTCGAGGGACAGCGCCAAGGAGAAGGCCAAGCAGCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8RHN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment