Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGT87_RS11495 Genome accession   NZ_CP152071
Coordinates   1509173..1510210 (-) Length   345 a.a.
NCBI ID   WP_353495474.1    Uniprot ID   -
Organism   Vibrio cholerae strain Sudan_2019_18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504173..1515210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT87_RS11470 (AAGT87_11475) - 1504644..1505207 (+) 564 WP_001054768.1 YqgE/AlgH family protein -
  AAGT87_RS11475 (AAGT87_11480) ruvX 1505288..1505710 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  AAGT87_RS11480 (AAGT87_11485) tyrS 1505748..1507028 (-) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  AAGT87_RS11485 (AAGT87_11490) - 1507137..1507934 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  AAGT87_RS11490 (AAGT87_11495) pilU 1508052..1509158 (-) 1107 WP_341260039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGT87_RS11495 (AAGT87_11500) pilT 1509173..1510210 (-) 1038 WP_353495474.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGT87_RS11500 (AAGT87_11505) - 1510236..1510946 (+) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGT87_RS11505 (AAGT87_11510) proC 1510999..1511817 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  AAGT87_RS11510 (AAGT87_11515) - 1511871..1512428 (+) 558 WP_001087263.1 YggT family protein -
  AAGT87_RS11515 (AAGT87_11520) yggU 1512428..1512718 (+) 291 WP_000718491.1 DUF167 family protein YggU -
  AAGT87_RS11520 (AAGT87_11525) - 1512764..1513195 (+) 432 WP_353495169.1 DUF4426 domain-containing protein -
  AAGT87_RS11525 (AAGT87_11530) - 1513265..1513867 (+) 603 WP_353495170.1 XTP/dITP diphosphatase -
  AAGT87_RS11530 (AAGT87_11535) hemW 1513861..1515036 (+) 1176 WP_000957351.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38305.91 Da        Isoelectric Point: 6.5572

>NTDB_id=987121 AAGT87_RS11495 WP_353495474.1 1509173..1510210(-) (pilT) [Vibrio cholerae strain Sudan_2019_18]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETDHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATLAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=987121 AAGT87_RS11495 WP_353495474.1 1509173..1510210(-) (pilT) [Vibrio cholerae strain Sudan_2019_18]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGCTAGA
GCAACTGGATGCTCCTGAAATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACTGCGGCAGAAACCGATCACTTGGTATTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTTGCGTGTCATGAAATCATGCTGGCGACGCTAGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCGCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.42

100

0.994
  pilT Vibrio cholerae strain A1552

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

71.131

97.391

0.693
  pilT Acinetobacter baumannii strain A118

71.131

97.391

0.693
  pilT Acinetobacter nosocomialis M2

70.833

97.391

0.69
  pilT Acinetobacter baylyi ADP1

71.818

95.652

0.687
  pilT Pseudomonas stutzeri DSM 10701

68.249

97.681

0.667
  pilT Pseudomonas aeruginosa PAK

66.176

98.551

0.652
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

63.939

95.652

0.612
  pilT Neisseria gonorrhoeae MS11

63.636

95.652

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.376

94.783

0.487
  pilU Vibrio cholerae strain A1552

41.617

96.812

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.237

97.971

0.394
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371

Multiple sequence alignment