Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGT87_RS11490 Genome accession   NZ_CP152071
Coordinates   1508052..1509158 (-) Length   368 a.a.
NCBI ID   WP_341260039.1    Uniprot ID   -
Organism   Vibrio cholerae strain Sudan_2019_18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1503052..1514158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGT87_RS11465 (AAGT87_11470) gshB 1503652..1504608 (+) 957 WP_000593313.1 glutathione synthase -
  AAGT87_RS11470 (AAGT87_11475) - 1504644..1505207 (+) 564 WP_001054768.1 YqgE/AlgH family protein -
  AAGT87_RS11475 (AAGT87_11480) ruvX 1505288..1505710 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  AAGT87_RS11480 (AAGT87_11485) tyrS 1505748..1507028 (-) 1281 WP_001088155.1 tyrosine--tRNA ligase -
  AAGT87_RS11485 (AAGT87_11490) - 1507137..1507934 (-) 798 WP_001123226.1 helix-turn-helix transcriptional regulator -
  AAGT87_RS11490 (AAGT87_11495) pilU 1508052..1509158 (-) 1107 WP_341260039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGT87_RS11495 (AAGT87_11500) pilT 1509173..1510210 (-) 1038 WP_353495474.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGT87_RS11500 (AAGT87_11505) - 1510236..1510946 (+) 711 WP_001256653.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGT87_RS11505 (AAGT87_11510) proC 1510999..1511817 (+) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  AAGT87_RS11510 (AAGT87_11515) - 1511871..1512428 (+) 558 WP_001087263.1 YggT family protein -
  AAGT87_RS11515 (AAGT87_11520) yggU 1512428..1512718 (+) 291 WP_000718491.1 DUF167 family protein YggU -
  AAGT87_RS11520 (AAGT87_11525) - 1512764..1513195 (+) 432 WP_353495169.1 DUF4426 domain-containing protein -
  AAGT87_RS11525 (AAGT87_11530) - 1513265..1513867 (+) 603 WP_353495170.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41319.32 Da        Isoelectric Point: 6.5076

>NTDB_id=987120 AAGT87_RS11490 WP_341260039.1 1508052..1509158(-) (pilU) [Vibrio cholerae strain Sudan_2019_18]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGAIGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=987120 AAGT87_RS11490 WP_341260039.1 1508052..1509158(-) (pilU) [Vibrio cholerae strain Sudan_2019_18]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
CAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAATCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCTAACAATGCTAACCAAGCGTTAGAGCGGATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.484

84.239

0.383
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Vibrio cholerae strain A1552

41.916

90.761

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

90.761

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Acinetobacter baylyi ADP1

42.154

88.315

0.372

Multiple sequence alignment