Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NSQ95_RS11620 Genome accession   NZ_CP151993
Coordinates   2379885..2380925 (-) Length   346 a.a.
NCBI ID   WP_342559663.1    Uniprot ID   -
Organism   Psychrobacillus sp. FSL W7-1457     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2374885..2385925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ95_RS11595 (NSQ95_11595) - 2375728..2376450 (-) 723 WP_342559658.1 hypothetical protein -
  NSQ95_RS11600 (NSQ95_11600) - 2376447..2377034 (-) 588 WP_342559659.1 hypothetical protein -
  NSQ95_RS11605 (NSQ95_11605) pilM 2377037..2378014 (-) 978 WP_342559660.1 pilus assembly protein PilM -
  NSQ95_RS11610 (NSQ95_11610) - 2378132..2378647 (-) 516 WP_342559661.1 type II secretion system protein -
  NSQ95_RS11615 (NSQ95_11615) - 2378680..2379885 (-) 1206 WP_342559662.1 type II secretion system F family protein -
  NSQ95_RS11620 (NSQ95_11620) pilT 2379885..2380925 (-) 1041 WP_342559663.1 type IV pilus twitching motility protein PilT Machinery gene
  NSQ95_RS11625 (NSQ95_11625) - 2380936..2382600 (-) 1665 WP_342559664.1 ATPase, T2SS/T4P/T4SS family -
  NSQ95_RS11630 (NSQ95_11630) - 2382622..2383929 (-) 1308 WP_342559665.1 VanW family protein -
  NSQ95_RS11635 (NSQ95_11635) - 2384181..2384582 (+) 402 WP_342559666.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NSQ95_RS11640 (NSQ95_11640) - 2384575..2385072 (+) 498 WP_342559667.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38266.84 Da        Isoelectric Point: 6.1751

>NTDB_id=983981 NSQ95_RS11620 WP_342559663.1 2379885..2380925(-) (pilT) [Psychrobacillus sp. FSL W7-1457]
MDLNIFNLLEKAYQDKASDLHLTVGLAPVFRVHGKLENFGDYQLQNNDTKKMLENLLDTDALDEFSQKGEIDFSFALEDL
CRFRINAYLQQNNVAIAIRIIESIIPSVEDLGMPNVLYTLADKPQGLILVTGPTGSGKSTTLAAMIDYINATKSKHIITL
EDPIEYVHRHKKSIVNQREVGSDTQSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIVSQRLFSKVNGSGRVVATEILIQTPSVANLIRNEKIHQIKNVMQTSRALGMHTLE
SSIQQLLVSKQISLDAAKPFMNAGEF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=983981 NSQ95_RS11620 WP_342559663.1 2379885..2380925(-) (pilT) [Psychrobacillus sp. FSL W7-1457]
ATGGATTTAAATATTTTTAACTTATTGGAAAAAGCATATCAAGATAAAGCATCTGATCTTCATTTAACAGTTGGCCTAGC
GCCGGTTTTTAGAGTTCATGGGAAGCTTGAAAACTTTGGAGATTACCAGTTGCAGAATAATGACACTAAAAAAATGCTGG
AAAATCTGTTAGATACGGATGCTCTGGATGAGTTTTCTCAAAAAGGAGAGATAGACTTTAGTTTTGCGTTAGAGGACCTT
TGCCGATTTCGAATCAATGCGTATCTCCAACAAAATAATGTTGCCATTGCTATCCGAATTATTGAATCTATCATACCTTC
TGTTGAAGATTTAGGAATGCCAAACGTCCTTTACACACTTGCGGACAAGCCACAAGGCCTTATTCTTGTCACTGGACCTA
CCGGATCAGGGAAATCTACAACACTAGCAGCGATGATTGATTATATTAATGCAACAAAATCAAAACATATTATTACGCTT
GAAGATCCGATTGAATATGTTCATAGACATAAGAAATCTATCGTCAATCAAAGAGAGGTTGGTTCGGATACCCAAAGTTT
TGCGAATGGATTGCGTGCAGCGCTGCGTCAAGATCCTGATGTAATTCTAGTAGGAGAAATGCGAGATTTAGAAACTATCT
CTACGGCGATTACAGCAGCTGAAACGGGACATTTAGTAATGGCAACATTGCATACTAGTAGCGCACCAACTACAATTGAT
CGTATTATTGACGTTTTTCCGCCTCATCAGCAAGGACAAATTCGTATCCAATTAGCGAATGTTTTACAGGGGATTGTGTC
ACAACGTCTTTTCTCAAAGGTTAACGGATCAGGAAGGGTTGTAGCTACAGAAATACTAATACAAACCCCATCGGTTGCAA
ACCTTATTCGTAACGAGAAGATTCATCAAATAAAAAATGTGATGCAAACTAGTCGTGCTTTAGGGATGCATACGCTGGAA
TCATCCATTCAGCAGCTCCTTGTTTCAAAACAAATCTCATTGGATGCAGCCAAGCCGTTTATGAATGCAGGTGAATTTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.952

96.243

0.5
  pilT Pseudomonas stutzeri DSM 10701

49.702

97.11

0.483
  pilT Legionella pneumophila strain Lp02

48.968

97.977

0.48
  pilT Legionella pneumophila strain ERS1305867

48.968

97.977

0.48
  pilT Neisseria gonorrhoeae MS11

48.512

97.11

0.471
  pilT Neisseria meningitidis 8013

48.512

97.11

0.471
  pilT Pseudomonas aeruginosa PAK

48.214

97.11

0.468
  pilT Acinetobacter baylyi ADP1

46.939

99.133

0.465
  pilT Acinetobacter baumannii D1279779

47.353

98.266

0.465
  pilT Acinetobacter nosocomialis M2

47.353

98.266

0.465
  pilT Acinetobacter baumannii strain A118

47.353

98.266

0.465
  pilT Vibrio cholerae strain A1552

48.615

93.931

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.615

93.931

0.457
  pilU Vibrio cholerae strain A1552

43.544

96.243

0.419
  pilU Pseudomonas stutzeri DSM 10701

41.818

95.376

0.399
  pilU Acinetobacter baylyi ADP1

40.176

98.555

0.396

Multiple sequence alignment